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    Compositions and techniques for nucleic acid primer extension
    22.
    发明授权
    Compositions and techniques for nucleic acid primer extension 有权

    公开(公告)号：US11884971B2

    公开(公告)日：2024-01-30

    申请号：US17391423

    申请日：2021-08-02

    申请人： Pacific Biosciences of California, Inc.

    发明人： Denis Malyshev ,  Arnold Oliphant

    IPC分类号： C12Q1/6853 ,  C12Q1/6869

    CPC分类号： C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2522/101 ,  C12Q2535/122 ,  C12Q2565/607

    摘要： A method of characterizing a nucleic acid that includes steps of (a) contacting a primer-template nucleic acid hybrid with a polymerase and a mixture of nucleotides to produce an extended primer hybrid and to form a stabilized ternary complex including the extended primer hybrid, the polymerase and a nucleotide cognate of the next base in the template, wherein the mixture contains nucleotide cognates of four different base types, wherein the nucleotide cognate of the first base type has a reversible terminator, and wherein nucleotide cognates of the second, third and fourth base types are extendable; (b) detecting the stabilized ternary complex to distinguish the next base from other base types in the template; and (c) determining the presence of a base multiplet in the template nucleic acid, the base multiplet including the first base type followed by the next base.

    Compositions and methods for nucleic acid amplification
    28.
    发明授权
    Compositions and methods for nucleic acid amplification 有权

    公开(公告)号：US11827925B2

    公开(公告)日：2023-11-28

    申请号：US17139127

    申请日：2020-12-31

    申请人： AMPLIWISE INC.

    发明人： Kai Wu ,  Mindy Su ,  Xing Su

    IPC分类号： C12Q1/6853 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/686

    CPC分类号： C12Q1/6853 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6827

    摘要： An embodiment relates to system comprising: (i) a reaction mixture comprises: a) a target nucleic acid molecule; b) a forward primer complementary to a strand of the target nucleic acid molecule, the forward primer comprises a first molecular moiety at a 3′ end, wherein the first molecular moiety is non-complementary to the strand of the target nucleic acid molecule; c) a reverse primer complementary to a complementary sequence of the strand of the target nucleic acid molecule, the reverse primer comprises a second molecular moiety at a 3′ end, wherein the second molecular moiety is non-complementary to the complementary sequence of the strand of the target nucleic acid molecule; d) a polymerase with 3′-5′ exonuclease activity; and (ii a device suitable of detecting an amplification products.

    METHOD FOR POSITIVE CONTROL REACTION USING PRE-POSITIVE CONTROL COMPOSITION
    29.
    发明公开
    METHOD FOR POSITIVE CONTROL REACTION USING PRE-POSITIVE CONTROL COMPOSITION 审中-公开

    公开(公告)号：US20230332217A1

    公开(公告)日：2023-10-19

    申请号：US18023105

    申请日：2021-08-27

    申请人： SEEGENE, INC.

    发明人： Jihoon PARK ,  Soyoung KIM ,  Dong-Min KIM ,  Ohsung KO

    IPC分类号： C12Q1/6853

    CPC分类号： C12Q1/6853 ,  C12Q2600/166 ,  C12Q2600/16

    摘要： Disclosed herein is a method for a positive control reaction using a pre-positive control composition. Unlike conventional positive controls, the pre-positive control composition according to the present disclosure is provided in the pre-positive control composition form, but not in a complete positive control form, until an experiment preparation stage, and can produce a complete-positive control through a positive control reaction, whereby the contamination that could occur in an experiment preparation stage for positive control preparation and positive control reactions can be minimize and an examination can be made to see whether the contamination comes from the positive control or not.

    Methods and Systems of Producing Single Stranded DNA via PCR Using Biotin-Labeled Primers
    30.
    发明公开
    Methods and Systems of Producing Single Stranded DNA via PCR Using Biotin-Labeled Primers 审中-公开

    公开(公告)号：US20230304083A1

    公开(公告)日：2023-09-28

    申请号：US18095785

    申请日：2023-01-11

    申请人： Applied DNA Sciences, Inc.

    发明人： Yuhua Sun ,  Ming-Hwa Liang

    IPC分类号： C12Q1/686 ,  C12Q1/6853

    CPC分类号： C12Q1/686 ,  C12Q1/6853

    摘要： The present invention provides methods of producing single-stranded DNA (ssDNA) from a double-stranded DNA (dsDNA) template. The method includes selecting either the (+)-strand or the (−)-strand of a dsDNA template as a target ssDNA, wherein if the target ssDNA is the (+)-strand of the dsDNA template, a biotin label is added onto the reverse primer, and wherein if the target ssDNA is the (−)-strand of the dsDNA template, a biotin label is added onto the forward primer. Next PCR is performed to produce biotinylated dsDNA fragments. Once the PCR is terminated, the biotinylated dsDNA fragments are purified. Next the target ssDNA is separated from the biotinylated dsDNA by immobilizing the biotinylated dsDNA onto a surface and precipitating the target ssDNA.