专利检索 ap:("MULTIPLEXDX, S.R.O.") AND inv:"Nina VOJTA;{hacek over (S)};{hacek over (S)}ÁKOVÁ" 第 1 页

1.

发明公开
LOOP MEDIATED ISOTHERMAL NUCLEIC ACID AMPLIFICATION (LAMP) USING LNA-MODIFIED PRIMERS AND A METAL-BASED COLORIMETRIC METHOD FOR DETECTING AN AMPLIFICATION PRODUCT 审中-公开

公开(公告)号：US20240026435A1

公开(公告)日：2024-01-25

申请号：US18252489

申请日：2021-11-10

申请人： MULTIPLEXDX, S.R.O.

发明人： Pavol CEKAN , Evan PAUL , Adrián SZOBI , Nina VOJTA;{hacek over (S)};{hacek over (S)}ÁKOVÁ , Katarina BURANOVSKÁ

IPC分类号： C12Q1/6853

CPC分类号： C12Q1/6853

摘要： The present invention relates to a loop mediated isothermal amplification (LAMP) method characterized in that the F3 nucleotide sequence and/or said B3 nucleotide sequence comprises one or more locked nucleic acid (LNA) nucleotides which are located within the first third of said F3 nucleotide sequence or said B3 nucleotide sequence, respectively. The present invention further relates to an in vitro method for detecting a nucleic acid sequence amplification product characterized by the use of a metallochromic indicator, preferably 5-Br-PAPS, and metal ions, preferably Zn2+ ions. The present invention further relates to a kit for carrying out the methods of the invention and a use of the kit.