公开(公告)号：US20240271087A1

公开(公告)日：2024-08-15

申请号：US18404213

申请日：2024-01-04

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Junying Yu

IPC: C12N5/074 ,  C12N5/0735

CPC classification number: C12N5/0607 ,  C12N5/0696 ,  C12N5/0606 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/99 ,  C12N2510/00 ,  C12N2799/027

Abstract: The present invention relates to methods for reprogramming a somatic call to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

公开(公告)号：US11566229B2

公开(公告)日：2023-01-31

申请号：US16557404

申请日：2019-08-30

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: C12N5/071 ,  A61K35/407

Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.

公开(公告)号：US20220090009A1

公开(公告)日：2022-03-24

申请号：US17540023

申请日：2021-12-01

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Jue Zhang

IPC: C12N5/071

Abstract: The present invention relates to chemically defined and xenogeneic material-free methods for deriving endothelial cells from human pluripotent stem cells. In particular, the present invention provides highly efficient and reproducible methods of obtaining human endothelial cells from human pluripotent stem cells, where endothelial cells derived from the methods provided herein are suitable for clinically relevant therapeutic applications.

公开(公告)号：US10760057B2

公开(公告)日：2020-09-01

申请号：US16029068

申请日：2018-07-06

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Jue Zhang

IPC: A61K31/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/077 ,  A61K31/4375 ,  C12N5/0735

Abstract: Methods of using a small molecule MYH11 agonist to inhibit intimal hyperplasia and to maintain a contractile phenotype in vitro and in vivo are described. Also described herein are methods for generating human contractile smooth muscle cells from human pluripotent stem cells under defined conditions in the presence of the small molecule MYH11 agonist.

公开(公告)号：US10106772B2

公开(公告)日：2018-10-23

申请号：US13793594

申请日：2013-03-11

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Junying Yu

IPC: C12N15/00 ,  C12N5/074 ,  C12N5/0735

Abstract: The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

公开(公告)号：US09644186B2

公开(公告)日：2017-05-09

申请号：US15063046

申请日：2016-03-07

Applicant: Wisconsin Alumni Research Foundation

Inventor： Guokai Chen ,  James A. Thomson

IPC: C12N5/00 ,  C12N5/074 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2501/845 ,  C12N2501/998

Abstract: Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.

公开(公告)号：US20160186146A1

公开(公告)日：2016-06-30

申请号：US14986363

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071

CPC classification number: C12N5/0697 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2502/086 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2533/30 ,  C12N2533/90 ,  C12Q1/6883 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/5058

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

Abstract translation: 本发明涉及三维（3D）组织构建体和使用这种3D组织构建体来筛选神经毒性试剂的方法。 特别地，本文提供了生产和使用包含生理相关的人细胞的复杂的高度均匀的人组织模型的方法，其中组织模型具有用于定量高通量筛选应用所需的样品均匀度和再现性的程度。

公开(公告)号：US20160186137A1

公开(公告)日：2016-06-30

申请号：US14986224

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/079 ,  C12N5/071 ,  G01N33/50 ,  C12N5/0789

CPC classification number: C12N5/0622 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0645 ,  C12N5/0647 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/22 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2513/00 ,  G01N33/5014 ,  G01N33/5058

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

Abstract translation: 本发明涉及从人多能干细胞衍生人类造血祖细胞，原始巨噬细胞和小胶质细胞的方法。 特别地，本文提供的是从人多能干细胞获得人原始巨噬细胞和小胶质细胞的高效和可重复的方法，其中原始巨噬细胞和小胶质细胞可适用于临床相关的治疗应用。

公开(公告)号：US20150368610A1

公开(公告)日：2015-12-24

申请号：US14818779

申请日：2015-08-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Zhonggang Hou ,  Guokai Chen

IPC: C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0606 ,  C07K14/78 ,  C12N5/0696 ,  C12N2500/02 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2533/50 ,  C12N2533/52

Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.

Abstract translation: 提供了来自Vitronectin的细胞培养物底物及其使用培养多能干细胞的方法。

公开(公告)号：US09023644B2

公开(公告)日：2015-05-05

申请号：US13717055

申请日：2012-12-17

Applicant: Wisconsin Alumni Research Foundation

Inventor： Guokai Chen ,  James A. Thomson

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/91 ,  C12N2506/02 ,  C12N2506/1307

Abstract: Methods are provided that exploit thermostable FGF-1 proteins for support of human pluripotent stem cell cultures. Also provided are compositions containing thermostable FGF-1 for culturing of human pluripotent stem cells.

Abstract translation: 提供了利用热稳定性FGF-1蛋白质来支持人多能干细胞培养物的方法。 还提供了含有用于培养人多能干细胞的耐热FGF-1的组合物。