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    Methine-substituted cyanine dye compounds
    21.
    发明申请
    Methine-substituted cyanine dye compounds 有权
    甲基取代的花青染料化合物

    公开(公告)号：US20050208534A1

    公开(公告)日：2005-09-22

    申请号：US11005861

    申请日：2004-12-06

    Applicant: Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

    Inventor： Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

    IPC: C07D413/02 ,  C07D417/02 ,  C07H21/04 ,  C12Q1/68

    CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

    Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

    Abstract translation: 具有作为核酸污渍的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物：其中R 1是C 1 -C 3烷基， 烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1至C 6烷基， -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选地是烷基，芳基，杂芳基，环状或杂环部分 被烷基，氨基，氨基烷基，羧基，硝基或卤素取代; 而其余的R 3，R 4或R 5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

    Modified actinomycin-based nucleic acid stains and methods of their use
    22.
    发明授权
    Modified actinomycin-based nucleic acid stains and methods of their use 有权
    改良的基于放线菌素的核酸污渍及其使用方法

    公开(公告)号：US08653261B2

    公开(公告)日：2014-02-18

    申请号：US13126150

    申请日：2009-10-26

    Applicant: Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

    Inventor： Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

    IPC: C07D265/38

    CPC classification number: C07D265/38

    Abstract: Actinomysin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided. The actinomysin-based near IR emitting compounds have the structure: wherein R is H or NH2; R1, R2, R3, and R4 are independently a moiety comprising 1-30 atoms selected from H, O, C, and N, wherein the atoms are in a linear, branched, or cyclic configuration; R3 and/or R4 comprise a quaternary nitrogen atom; and R5 is H, F, or Cl.

    Abstract translation: 提供了基于放射性肌动蛋白的近红外发射化合物及其用作核酸污渍的方法。 基于放电肌注蛋白的近红外发射化合物具有以下结构：其中R是H或NH 2; R1，R2，R3和R4独立地是包含1-30个选自H，O，C和N的原子的部分，其中原子是直链，支链或环状构型; R3和/或R4包含季氮原子; R5为H，F或Cl。

    Unsymmetrical cyanine dimer compounds and their application
    24.
    发明授权
    Unsymmetrical cyanine dimer compounds and their application 有权
    不对称花青二聚体化合物及其应用

    公开(公告)号：US08241852B2

    公开(公告)日：2012-08-14

    申请号：US13017242

    申请日：2011-01-31

    Applicant: Stephen Yue ,  Ching-Ying Cheung

    Inventor： Stephen Yue ,  Ching-Ying Cheung

    IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/02

    CPC classification number: C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

    Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

    Abstract translation: 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。

    CYANINE COMPOUNDS AND THEIR APPLICATION AS QUENCHING COMPOUNDS
    27.
    发明申请
    CYANINE COMPOUNDS AND THEIR APPLICATION AS QUENCHING COMPOUNDS 审中-公开
    氰胺化合物及其作为化学化合物的应用

    公开(公告)号：US20110105362A1

    公开(公告)日：2011-05-05

    申请号：US13005002

    申请日：2011-01-12

    Applicant: Rosaria P. Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    Inventor： Rosaria P. Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    IPC: C40B40/04 ,  C12N9/96 ,  C12N7/00 ,  C12N1/00 ,  C07K2/00 ,  C07K14/00 ,  C07K16/00 ,  C07H21/00 ,  C07H19/00 ,  C07D209/56 ,  C07D209/18

    CPC classification number: C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

    Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

    Abstract translation: 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。

    DETECTION OF IMMOBILIZED NUCLEIC ACID
    29.
    发明申请
    DETECTION OF IMMOBILIZED NUCLEIC ACID 有权

    公开(公告)号：US20100216665A1

    公开(公告)日：2010-08-26

    申请号：US12771897

    申请日：2010-04-30

    Applicant: Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    Inventor： Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    IPC: C40B30/04 ,  G01N33/00

    CPC classification number: G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    Methine-substituted cyanine dye compounds
    30.
    发明授权
    Methine-substituted cyanine dye compounds 有权
    甲基取代的花青染料化合物

    公开(公告)号：US07776529B2

    公开(公告)日：2010-08-17

    申请号：US11005861

    申请日：2004-12-06

    Applicant: Jason Alfred Dallwig ,  David Carl Hagen ,  Ching-Ying Cheung ,  Gerald Alan Thomas ,  Stephen Yue

    Inventor： Jason Alfred Dallwig ,  David Carl Hagen ,  Ching-Ying Cheung ,  Gerald Alan Thomas ,  Stephen Yue

    IPC: A61K31/4709 ,  C07D215/12 ,  C12Q1/68

    CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

    Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

    Abstract translation: 具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

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