公开(公告)号：US11786898B2

公开(公告)日：2023-10-17

申请号：US16818953

申请日：2020-03-13

申请人： Illumina, Inc.

发明人： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC分类号： B01L3/00 ,  B01L7/00 ,  F16K99/00 ,  G01N35/08 ,  C12Q1/6869

CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502715 ,  B01L7/52 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  G01N35/08 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0084

摘要： Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

公开(公告)号：US20230184711A1

公开(公告)日：2023-06-15

申请号：US18003493

申请日：2021-06-18

申请人： Illumina, Inc.

发明人： Boyan Boyanov ,  Rico Otto ,  Jeffrey G. Mandell

IPC分类号： G01N27/414

CPC分类号： G01N27/4146

摘要： Devices and methods of using the devices are disclosed which can provide scalability, improved sensitivity and reduced noise for sequencing polynucleotide. Examples of the devices include a biological or solid-state nanopore, a field effect transistor (FET) sensor with improved gate controllability over the channel, and a porous structure.

公开(公告)号：US20230145389A1

公开(公告)日：2023-05-11

申请号：US18087499

申请日：2022-12-22

申请人： ILLUMINA, INC.

发明人： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC分类号： B01L3/00 ,  F16K99/00 ,  B01L7/00

CPC分类号： B01L3/502715 ,  B01L3/502738 ,  B01L7/525 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/065 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0074 ,  F16K2099/0084

摘要： Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US11629373B2

公开(公告)日：2023-04-18

申请号：US16832309

申请日：2020-03-27

申请人： ILLUMINA, INC.

发明人： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC分类号： C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

摘要： Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20230112203A1

公开(公告)日：2023-04-13

申请号：US17934175

申请日：2022-09-21

申请人： ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

发明人： Boyan Boyanov ,  Craig Ciesla ,  Anthony Flannery ,  Gerald Kreindl ,  Rean Silke Musa ,  Darren Segale

IPC分类号： G01N33/487 ,  C12Q1/6869 ,  G01N27/414 ,  B01L3/00

摘要： Devices for sequencing biopolymers and methods of using the devices are disclosed. In one example, such a device has a nanopore, a plurality of wells and fluidic tunnels to allow a biopolymer to translocate in the device. In some embodiments, the device may include integrated electronics or micro-electromechanical systems, such as valves, bubble generators/annihilators or pressure pulse generators, to actively control fluidic/ionic/electric flows in the device.

公开(公告)号：US10605766B2

公开(公告)日：2020-03-31

申请号：US14798762

申请日：2015-07-14

申请人： ILLUMINA, INC.

发明人： Boyan Boyanov ,  Jeffrey G. Mandell ,  Jingwei Bai ,  Kevin L. Gunderson ,  Cheng-Yao Chen ,  Michel Perbost

IPC分类号： C12Q1/68 ,  G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825 ,  B82Y15/00

摘要： A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US10551342B2

公开(公告)日：2020-02-04

申请号：US16024299

申请日：2018-06-29

申请人： ILLUMINA, INC.

发明人： Boyan Boyanov

IPC分类号： H01L21/02 ,  G01N27/414 ,  C12Q1/6869 ,  G01R19/00 ,  H03K3/03

摘要： Apparatus and methods are disclosed for single molecule field effect sensors having conductive channels functionalized with a single active moiety. A region of a nanostructure (e.g., such as a silicon nanowire or a carbon nanotube) provide the conductive channel. Trapped state density of the nanostructure is modified for a portion of the nanostructure in proximity with a location where the active moiety is linked to the nanostructure. In one example, the semiconductor device includes a source, a drain, a channel including a nanostructure having a modified portion with an increased trap state density, the modified portion being further functionalized with an active moiety. A gate terminal is in electrical communication with the nanostructure. As a varying electrical signal is applied to an ionic solution in contact with the nanostructure channel, changes in current observed from the semiconductor device can be used to identify composition of the analyte.

公开(公告)号：US20240293816A1

公开(公告)日：2024-09-05

申请号：US18574214

申请日：2022-06-30

申请人： ILLUMINA, INC.

发明人： Rean Silke Musa ,  Anthony Flannery ,  Boyan Boyanov ,  Nigel COBURN ,  Sharis MINASSIAN

IPC分类号： B01L3/00 ,  C12Q1/6869

CPC分类号： B01L3/502761 ,  C12Q1/6869 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2400/0427 ,  B01L2400/046

摘要： Devices for sequencing biopolymers, methods of manufacturing the devices, and methods of using the devices are disclosed. In one example, such a device has a nanopore and a horizontal nanochannel. In some embodiments, the horizontal nanochannel may take a tortuous path. In some embodiments, such a device includes gas or air bubble generators or pressure pulse generators to block or unblock the horizontal nanochannel.

公开(公告)号：US11680906B2

公开(公告)日：2023-06-20

申请号：US16607354

申请日：2018-04-19

申请人： ILLUMINA, INC.

发明人： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC分类号： G01N21/64 ,  G01N21/77 ,  B01L3/00 ,  G01N15/14

CPC分类号： G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

摘要： An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20220299469A1

公开(公告)日：2022-09-22

申请号：US17828991

申请日：2022-05-31

申请人： Illumina, Inc.

发明人： Boyan Boyanov ,  Jens Gundlach

IPC分类号： G01N27/414 ,  G01N33/487 ,  H01L21/74 ,  H01L29/66 ,  H01L29/786

摘要： Example devices include a cis well associated with a cis electrode, a trans well associated with a trans electrode, and a field effect transistor (FET) positioned between the cis well and the trans well. Examples of the field effect transistor (FET) include a fluidic system defined therein. The fluidic system includes a first cavity facing the cis well, a second cavity fluidically connected to the trans well, and a through via extending through the field effect transistor from the first cavity. A first nanoscale opening fluidically connects the cis well and the first cavity, the first nanoscale opening having an inner diameter. A second nanoscale opening fluidically connects the through via and the second cavity, the second nanoscale opening having an inner diameter. The second nanoscale opening inner diameter is larger than the first nanoscale opening inner diameter.