公开(公告)号：US20250044230A1

公开(公告)日：2025-02-06

申请号：US18922150

申请日：2024-10-21

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  B01L3/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N27/414 ,  G01N33/543 ,  H01L27/00 ,  H01L27/088 ,  H01L27/146

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US12140543B2

公开(公告)日：2024-11-12

申请号：US18339185

申请日：2023-06-21

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  B01L3/00 ,  G01N21/64 ,  G01N27/414 ,  G01N33/543 ,  H01L27/00 ,  H01L27/088 ,  C12Q1/6874 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11782006B2

公开(公告)日：2023-10-10

申请号：US16701747

申请日：2019-12-03

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Sergio Peisajovich ,  Jeffrey G. Mandell

IPC: G01N27/12 ,  G01N27/414 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N27/125 ,  C12Q1/68 ,  G01N27/128 ,  G01N27/4145 ,  G01N33/54366

Abstract: A sensing system includes a charge sensor including two electrodes and an electrically conductive channel connecting the two electrodes. The sensing system also includes a charged molecule attached to the electrically conductive channel. The charged molecule includes a recognition site to reversibly bind a label of a labeled nucleotide; has an unbound favored conformation associated with an unbound charge configuration; and has a favored conformation associated with a charge configuration when the recognition site is bound to the label. The charge configuration is different from the unbound charge configuration. The sensing system further includes a polymerase attached to the electrically conductive channel or to the charged molecule.

公开(公告)号：US11719637B2

公开(公告)日：2023-08-08

申请号：US17405379

申请日：2021-08-18

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  B01L3/00 ,  G01N33/543 ,  G01N21/64 ,  H01L27/088 ,  H01L29/78 ,  G01N21/05 ,  C12Q1/6874

CPC classification number: G01N21/645 ,  B01L3/502707 ,  B01L3/502715 ,  G01N21/6428 ,  G01N21/6454 ,  G01N27/4145 ,  G01N33/54373 ,  H01L27/00 ,  H01L27/088 ,  H01L27/14623 ,  C12Q1/6874 ,  G01N21/05 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11680292B2

公开(公告)日：2023-06-20

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20230002822A1

公开(公告)日：2023-01-05

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210381977A1

公开(公告)日：2021-12-09

申请号：US17405379

申请日：2021-08-18

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  G01N33/543 ,  B01L3/00 ,  H01L27/146 ,  H01L27/088 ,  H01L27/00 ,  G01N27/414

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US20210310985A1

公开(公告)日：2021-10-07

申请号：US17257464

申请日：2020-01-24

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Kaitlin M. Pugliese ,  Jeffrey G. Mandell ,  Maria Candelaria Rogert Bacigalupo ,  Boyan Boyanov

IPC: G01N27/414 ,  C12Q1/00 ,  C12Q1/48 ,  C12Q1/6825

Abstract: In an example, a sensing system includes a pH sensor. The pH sensor includes two electrodes and a conductive channel operatively connected to the two electrodes. A complex is attached to the conductive channel of the pH sensor. The complex includes a polymerase linked to at least one pH altering moiety that is to participate in generating a pH change within proximity of the conductive channel from consumption of a secondary substrate in a fluid that is exposed to the pH sensor. The at least one pH altering moiety is selected from the group consisting of an enzyme, a metal coordination complex, a co-factor, and an activator.

公开(公告)号：US11125691B2

公开(公告)日：2021-09-21

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  G01N33/543 ,  G01N21/64 ,  B01L3/00 ,  H01L27/088 ,  C12Q1/6874 ,  H01L29/78 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11124829B2

公开(公告)日：2021-09-21

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/68 ,  G03F7/16 ,  C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.