公开(公告)号：US11946929B2

公开(公告)日：2024-04-02

申请号：US18354144

申请日：2023-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

CPC classification number: G01N33/54366 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  B01F33/3011 ,  B01F33/3021 ,  C40B30/04

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11754499B2

公开(公告)日：2023-09-12

申请号：US16780508

申请日：2020-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: B01L3/00 ,  G01N21/64 ,  B01J19/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  B01L7/00

CPC classification number: G01N21/6445 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01J2219/0059 ,  B01J2219/0065 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N2500/00 ,  C12Q1/6827 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/131 ,  C12Q2563/159 ,  C12Q1/6804 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/159 ,  C12Q1/6851 ,  C12Q2563/159 ,  C12Q2561/113 ,  C12Q2565/1015

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.

公开(公告)号：US20230220447A1

公开(公告)日：2023-07-13

申请号：US18178121

申请日：2023-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6804 ,  G01N33/543 ,  G01N33/58 ,  C40B50/08

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12N15/1075 ,  C12Q1/6804 ,  G01N33/5436 ,  G01N33/58 ,  C40B50/08 ,  C12Q2521/10 ,  C12Q2565/30 ,  G01N2458/10 ,  C12Q2563/179

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11635427B2

公开(公告)日：2023-04-25

申请号：US16937908

申请日：2020-07-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011 ,  B01F33/302 ,  C40B70/00

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11596908B2

公开(公告)日：2023-03-07

申请号：US16105042

申请日：2018-08-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  John Brian Hutchison ,  Michael L. Samuels ,  Michael Weiner

IPC: B01F3/08 ,  G01N33/50 ,  C40B50/08 ,  C40B40/04 ,  B01F23/41 ,  B01J19/00 ,  B01L3/00 ,  G01N15/14 ,  B01F25/433 ,  B01F33/3011 ,  B01L7/00 ,  G01N15/10 ,  B01F101/23

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US11511242B2

公开(公告)日：2022-11-29

申请号：US17510092

申请日：2021-10-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  John Brian Hutchison ,  Michael L. Samuels ,  Michael Weiner

IPC: B01F3/08 ,  B01F5/06 ,  B01J19/00 ,  B01F23/41 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  G01N33/50 ,  G01N15/14 ,  B01F25/433 ,  B01F33/3011 ,  B01L7/00 ,  G01N15/10 ,  B01F101/23

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US20210395808A1

公开(公告)日：2021-12-23

申请号：US17466309

申请日：2021-09-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: C12Q1/6844 ,  C12Q1/6858

Abstract: The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

公开(公告)号：US11187702B2

公开(公告)日：2021-11-30

申请号：US17081220

申请日：2020-10-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/58 ,  B01L7/00 ,  G01N21/64

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US11174509B2

公开(公告)日：2021-11-16

申请号：US15947346

申请日：2018-04-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: C12Q1/6844 ,  C12Q1/6858

Abstract: The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

公开(公告)号：US10761090B2

公开(公告)日：2020-09-01

申请号：US15415276

申请日：2017-01-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/50 ,  G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F13/00

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.