公开(公告)号：US10067121B2

公开(公告)日：2018-09-04

申请号：US14541024

申请日：2014-11-13

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  C12Q1/00 ,  G01N27/02 ,  G01N33/543

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: Use of cell-substrate impedance based methods for screening for agonists of G-Protein Coupled Receptors (GPCRs) or inhibitors of a Receptor Tyrosine Kinases (RTKs), identifying compounds that affect GPCR or RTK pathways, validating molecular targets involved in a GPCR or RTK signaling pathways, monitoring dose-dependent functional activation of GPCR or RTK; determining desensitization of a GPCR and identifying a compound capable of affecting RTK activity in cancer cell proliferation.

公开(公告)号：US09623027B2

公开(公告)日：2017-04-18

申请号：US14712794

申请日：2015-05-14

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A01N43/90 ,  A61K31/519 ,  C07D487/00 ,  C07D487/04 ,  A61K45/06

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

公开(公告)号：US09612234B2

公开(公告)日：2017-04-04

申请号：US13109809

申请日：2011-05-17

Applicant: Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

Inventor： Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: C40B30/06 ,  G01N7/00 ,  C12Q1/00 ,  G01N33/50 ,  G01N33/483

CPC classification number: G01N33/48728 ,  G01N33/4836 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5061 ,  G01N33/5073 ,  G01N2800/326 ,  G01N2800/52

Abstract: A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.

公开(公告)号：US09586965B2

公开(公告)日：2017-03-07

申请号：US14420341

申请日：2013-07-11

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: C07D487/04 ,  A61K31/519 ,  A61K45/06 ,  A61P35/00 ,  A61P29/00 ,  A61P17/06

CPC classification number: C07D487/04 ,  A61K31/519 ,  A61K45/06 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of proliferation disorders and other diseases related to the dysregulation of kinase (such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, BTK, FLT3(D835Y), ITK, JAK1, JAK2, JAK3, TEC and TXK) and/or the respective pathways.

Abstract translation: 本发明涉及某些吡咯并嘧啶衍生物，含有它们的药物组合物及其使用方法，包括治疗增殖紊乱和与激酶失调相关的其它疾病的方法（例如但不限于EGFR（包括HER ），Alk，PDGFR，BLK，BMX / ETK，BTK，FLT3（D835Y），ITK，JAK1，JAK2，JAK3，TEC和TXK）和/或各自的途径。

公开(公告)号：US20150133457A1

公开(公告)日：2015-05-14

申请号：US14329890

申请日：2014-07-11

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao XU ,  Xiaobo WANG ,  Long MAO ,  Li ZHAO ,  Biao XI

IPC: C07D487/04 ,  C07D239/49 ,  C07D417/12 ,  C07D401/12 ,  C07D473/18 ,  C07D239/52 ,  C07D403/12

CPC classification number: C07D519/00 ,  C07D239/47 ,  C07D239/48 ,  C07D239/49 ,  C07D239/52 ,  C07D401/12 ,  C07D403/12 ,  C07D417/12 ,  C07D473/18 ,  C07D487/04

Abstract: The present invention relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment and/or prevention of a proliferation disorder, a cancer, a tumor, an inflammatory disease, an autoimmune disease, psoriasis, dry eye or an immunologically related disease, and in some embodiments diseases or disorders related to the dysregulation of kinase such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, FLT3(D835Y), ITK, TEC, TXK, BTK, or JAK, and the respective pathways.

Abstract translation: 本发明涉及药物化合物，组合物和方法，特别是因为它们与用于治疗和/或预防增殖病症，癌症，肿瘤，炎性疾病，自身免疫性疾病，牛皮癣，干性疾病的组合物和方法有关 眼睛或免疫相关疾病，在一些实施方案中，与激酶失调相关的疾病或病症，例如但不限于EGFR（包括HER），Alk，PDGFR，BLK，BMX / ETK，FLT3（D835Y），ITK ，TEC，TXK，BTK或JAK，以及各自的途径。

公开(公告)号：US08921041B2

公开(公告)日：2014-12-30

申请号：US13474355

申请日：2012-05-17

Applicant: Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC: C12Q1/68 ,  C12M1/42 ,  C12N15/87

CPC classification number: C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

Abstract translation: 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US20130200277A1

公开(公告)日：2013-08-08

申请号：US13657845

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC: G01N21/64

CPC classification number: G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

Abstract translation: 一种用于检测由多个激发源引起的光的信号分量的系统，包括：流路，包括至少两个空间分离的光询问区; 非调制激励源，其将第一波长的光束以接近恒定的强度引导到第一光学询问区上; 调制激励源，其将以调制频率随时间调制的第二波长的光束引导到第二光学询问区上; 检测器子系统，包括一组检测器，其被配置为检测从流过所述至少两个光询问区的颗粒发射的光并将检测到的光转换成总电信号; 以及处理器，其根据从每个光询问区检测的光来确定信号分量。

公开(公告)号：USD684703S1

公开(公告)日：2013-06-18

申请号：US29407358

申请日：2011-11-28

Applicant: Manfred Watzele ,  Alexander Seiler ,  Donald Thomas ,  Wei Li ,  Tianxing Wang ,  Nan Li ,  Xiaobo Wang

Designer： Manfred Watzele ,  Alexander Seiler ,  Donald Thomas ,  Wei Li ,  Tianxing Wang ,  Nan Li ,  Xiaobo Wang

公开(公告)号：US20130100443A1

公开(公告)日：2013-04-25

申请号：US13656457

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Ye Chen ,  Xiaobo Wang

IPC: G02B27/10 ,  G01J3/443

CPC classification number: G02B27/1006 ,  G01J3/14 ,  G01J3/36 ,  G01J3/443 ,  G02B5/04 ,  G02B5/285 ,  G02B27/141 ,  G02B27/142

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

Abstract translation: 一种棱镜，包括面以提供多个平坦表面的基板，其中，所述多个表面中的至少两个表面，每个包括过滤器涂层，形成至少两个过滤器表面，其中每个过滤器表面选择性地允许预定波长的通过并反射 沿着光路的剩余波长朝向多个表面中的另一个，可选地另外的滤光器表面，其中沿着光路的多个表面中的每一个的入射角相等或接近相等。 一种结合棱镜的装置及其用于将光谱分解成多个波长或波长范围的用途。

公开(公告)号：US08344742B2

公开(公告)日：2013-01-01

申请号：US13011725

申请日：2011-01-21

Applicant: Yama A. Abassi ,  Xiaobo Wang ,  Xiao Xu

Inventor： Yama A. Abassi ,  Xiaobo Wang ,  Xiao Xu

IPC: G01R27/08

CPC classification number: C12Q1/025 ,  C12Q1/002 ,  G01N27/02 ,  G01N33/4836 ,  G01N33/5014 ,  G01N33/5438

Abstract: The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis.

Abstract translation: 本发明提供了研究化合物作用机理的方法，其包括提供用于监测细胞 - 基底阻抗的装置; 将设备连接到阻抗分析仪; 将细胞添加到两个或更多个孔中; 向至少一个孔中加入测试化合物并提供至少一个对照孔; 监测井的阻抗以获得一系列阻抗测量; 绘制阻抗测量以获得阻抗曲线并比较阻抗曲线以确定测试化合物对细胞生长或行为具有显着影响的时间范围。 确定时间框架提供了响应于测试化合物的细胞状态变化的信息，包括细胞附着或粘附状态，细胞生长或增殖状态，活细胞或死细胞的数量，细胞骨架组织或结构以及细胞数目 通过凋亡或坏死。