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58 条记录 (耗时 0.027 秒)

    Methods for the reduction of stutter in microsatellite amplification
    12.
    发明申请
    Methods for the reduction of stutter in microsatellite amplification 有权
    减少微卫星放大的口吃的方法

    公开(公告)号：US20020187475A1

    公开(公告)日：2002-12-12

    申请号：US09850590

    申请日：2001-05-07

    发明人： Sulekha Rao Coticone ,  Will Bloch

    IPC分类号： C12Q001/68 ,  C12P019/34

    CPC分类号： C12Q1/6848 ,  C12Q2549/125 ,  C12Q2527/137 ,  C12Q2521/101

    摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a GnullC content of greater than 50%; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of sorbitol effective to reduce stutter relative to the amount of stutter observed in the absence of sorbitol. The invention also provides compositions containing sorbitol, kits for amplifying microsatellites having a GnullC content of greater than 50%, and methods of using all of the foregoing.

    摘要翻译： 本发明提供了减少微卫星扩增中的口吃的方法，包括提供包含G + C含量大于50％的微卫星样品的步骤; 使样品与至少一种具有核酸聚合酶活性的酶接触; 并将样品与酶温育足够的时间和足以扩增微卫星的条件; 其中在一定量的山梨糖醇的存在下进行培养，其有效地减少相对于在不存在山梨糖醇时观察到的口吃量的口吃。 本发明还提供含有山梨醇的组合物，用于扩增G + C含量大于50％的微卫星的试剂盒，以及使用上述所有方法。

    Decoy probes
    13.
    发明申请
    Decoy probes 失效
    诱饵探针

    公开(公告)号：US20020038016A1

    公开(公告)日：2002-03-28

    申请号：US09867193

    申请日：2001-05-29

    发明人： Christopher C. Adams ,  Steven T. Brentano ,  Gary P. Schroth

    IPC分类号： C07H021/04

    CPC分类号： C12Q1/6865 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2525/301 ,  C12Q2549/125

    摘要： The present invention features inhibitors of target-independent amplification and the use of such inhibitors for enhancing an amplification protocol. The inhibitors are believed to enhance an amplification protocol by inhibiting the ability of one or more nucleic acid polymerases to use nucleic acid in a polymerase reaction in the absence of target nucleic acid.

    摘要翻译： 本发明的特征在于靶依赖性扩增的抑制剂以及这种抑制剂用于增强扩增方案的用途。 认为抑制剂通过抑制一种或多种核酸聚合酶在不存在靶核酸的聚合酶反应中使用核酸的能力来增强扩增方案。

    NOVEL HOT START NUCLEIC ACID AMPLIFICATION
    17.
    发明申请
    NOVEL HOT START NUCLEIC ACID AMPLIFICATION 有权
    新的开始核酸放大

    公开(公告)号：US20110189736A1

    公开(公告)日：2011-08-04

    申请号：US13074752

    申请日：2011-03-29

    申请人： Christopher J. Kubu ,  Jeannine C. Muller-Greven ,  Robert B. Moffett

    发明人： Christopher J. Kubu ,  Jeannine C. Muller-Greven ,  Robert B. Moffett

    IPC分类号： C12P19/34 ,  C12N9/10

    CPC分类号： C12Q1/6848 ,  C12Q1/686 ,  C12Q2549/125 ,  C12Q2533/101 ,  C12Q2522/101 ,  C12Q2549/101

    摘要： Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.

    摘要翻译： 提供了用于进行核酸复制和扩增反应的方法和组合物。 在反应混合物中选择并提供单链核酸结合蛋白，所述反应混合物在低，非收缩温度下组装以包括用于成功的核酸复制或扩增反应的所有必需试剂。 通过在低温下将单链核酸结合蛋白并入反应混合物中，尽管反应混合物已经在非收敛温度下完全组装，但非特异性产物如扩增产物的产生得到改善。

    Apparatus and method for isolating a nucleic acid from a sample
    18.
    发明授权
    Apparatus and method for isolating a nucleic acid from a sample 失效
    从样品中分离核酸的装置和方法

    公开(公告)号：US07217513B2

    公开(公告)日：2007-05-15

    申请号：US10193742

    申请日：2002-07-10

    申请人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

    发明人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/0282 ,  B01L3/0241 ,  B01L3/5023 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/049 ,  C12Q1/6806 ,  C12Q2565/137 ,  C12Q2549/125

    摘要： A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.

    摘要翻译： 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触，多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体，由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离，由此制备用于扩增的核酸组分。 该方法可以在包括多孔载体的装置中进行，所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体，所述内部与 多孔载体，其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分，并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离，由此制备 用于扩增的核酸组分。

    Oligonucleotide sequestering agents and method of use
    19.
    发明申请
    Oligonucleotide sequestering agents and method of use 审中-公开
    寡核苷酸螯合剂及其使用方法

    公开(公告)号：US20070009894A1

    公开(公告)日：2007-01-11

    申请号：US10569766

    申请日：2004-08-23

    申请人： Donald Crothers

    发明人： Donald Crothers

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C12Q1/6846 ,  C12Q2549/125 ,  C12Q2533/107 ,  C12Q2525/301

    摘要： The present application provides methods and compositions for use in detecting the presence of a target nucleic acid in a sample. In some embodiments, the methods employ sequestering agents which specifically interact with complementary nucleic acids which will be ligated together if the target nucleic acid is present in the sample. Detection of a ligation product comprising the complementary nucleic acids indicates that the target nucleic acid is present in the sample.

    摘要翻译： 本申请提供了用于检测样品中靶核酸的存在的方法和组合物。 在一些实施方案中，所述方法使用与互补核酸特异性相互作用的螯合剂，如果靶核酸存在于样品中则将互连在一起。 包含互补核酸的连接产物的检测表明靶核酸存在于样品中。