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公开(公告)号:US20080131949A1
公开(公告)日:2008-06-05
申请号:US11804598
申请日:2007-05-18
申请人: Laura T. Bortolin , Lalitha Parameswaran , James Harper , Johanna Bobrow , Mark A. Hollis , Drew Chapman Brown , Eric Scott Clasen , John Calvin Schmidt
发明人: Laura T. Bortolin , Lalitha Parameswaran , James Harper , Johanna Bobrow , Mark A. Hollis , Drew Chapman Brown , Eric Scott Clasen , John Calvin Schmidt
CPC分类号: B01L3/502 , B01L2200/10 , B01L2200/16 , B01L2300/042 , B01L2300/043 , B01L2300/044 , B01L2300/047 , B01L2300/048 , B01L2300/0609 , B01L2300/0672 , B01L2300/0681 , B01L2300/069 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2400/043 , B01L2400/0644 , B01L2400/0683 , C07H21/04 , C12N15/1013
摘要: A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The sample is separated from raw sample components through means that include a magnetic substrate. An apparatus suitable for conducting the method of the invention also is described.
摘要翻译: 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触,多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体,由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离,由此制备用于扩增的核酸组分。 样品通过包括磁性底物的方式与原始样品组分分离。 还描述了适用于进行本发明的方法的装置。
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公开(公告)号:US07217513B2
公开(公告)日:2007-05-15
申请号:US10193742
申请日:2002-07-10
申请人: Lalitha Parameswaran , Albert Young , Laura T. Bortolin , Mark A. Hollis , James Harper , Johanna Bobrow
发明人: Lalitha Parameswaran , Albert Young , Laura T. Bortolin , Mark A. Hollis , James Harper , Johanna Bobrow
IPC分类号: C12Q1/68
CPC分类号: B01L3/0282 , B01L3/0241 , B01L3/5023 , B01L2400/0406 , B01L2400/0415 , B01L2400/049 , C12Q1/6806 , C12Q2565/137 , C12Q2549/125
摘要: A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.
摘要翻译: 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触,多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体,由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离,由此制备用于扩增的核酸组分。 该方法可以在包括多孔载体的装置中进行,所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体,所述内部与 多孔载体,其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分,并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离,由此制备 用于扩增的核酸组分。
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公开(公告)号:US08062846B2
公开(公告)日:2011-11-22
申请号:US11804598
申请日:2007-05-18
申请人: Laura T. Bortolin , Lalitha Parameswaran , James Harper , Johanna Bobrow , Mark A. Hollis , Drew Chapman Brown , Eric Scott Clasen , John Calvin Schmidt
发明人: Laura T. Bortolin , Lalitha Parameswaran , James Harper , Johanna Bobrow , Mark A. Hollis , Drew Chapman Brown , Eric Scott Clasen , John Calvin Schmidt
CPC分类号: B01L3/502 , B01L2200/10 , B01L2200/16 , B01L2300/042 , B01L2300/043 , B01L2300/044 , B01L2300/047 , B01L2300/048 , B01L2300/0609 , B01L2300/0672 , B01L2300/0681 , B01L2300/069 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2400/043 , B01L2400/0644 , B01L2400/0683 , C07H21/04 , C12N15/1013
摘要: An apparatus for preparing a nucleic acid component of a sample for amplification includes a porous support having an agent that deactivates a nucleic acid amplification inhibitor component of the sample. The apparatus further includes a housing with an opening and defining an interior. The interior of the housing is in fluid communication with the porous support, and at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support. The apparatus also includes a magnetic substrate for separating nucleic acid from a sample.
摘要翻译: 用于制备用于扩增的样品的核酸组分的装置包括具有使样品的核酸扩增抑制剂组分失活的试剂的多孔载体。 该装置还包括具有开口并限定内部的壳体。 壳体的内部与多孔支撑件流体连通,并且引导通过开口的流体的至少一部分被引导通过多孔支撑件的至少一部分。 该装置还包括用于从样品中分离核酸的磁性底物。
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