Apparatus and method for isolating a nucleic acid from a sample
    2.
    发明授权
    Apparatus and method for isolating a nucleic acid from a sample 失效
    从样品中分离核酸的装置和方法

    公开(公告)号:US07217513B2

    公开(公告)日:2007-05-15

    申请号:US10193742

    申请日:2002-07-10

    IPC分类号: C12Q1/68

    摘要: A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.

    摘要翻译: 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触,多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体,由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离,由此制备用于扩增的核酸组分。 该方法可以在包括多孔载体的装置中进行,所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体,所述内部与 多孔载体,其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分,并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离,由此制备 用于扩增的核酸组分。