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公开(公告)号：US07700281B2
公开(公告)日：2010-04-20
申请号：US11171008
申请日：2005-06-29
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2549/125 , C12Q2533/101 , C12Q2522/101 , C12Q2549/101
摘要： Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.
摘要翻译： 提供了用于进行核酸复制和扩增反应的方法和组合物。 在反应混合物中选择并提供单链核酸结合蛋白，所述反应混合物在低，非收缩温度下组装以包括用于成功的核酸复制或扩增反应的所有必需试剂。 通过在低温下将单链核酸结合蛋白并入反应混合物中，尽管反应混合物已经在非收敛温度下完全组装，但非特异性产物如扩增产物的产生得到改善。
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公开(公告)号：US20080138878A1
公开(公告)日：2008-06-12
申请号：US11939820
申请日：2007-11-14
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2549/125 , C12Q2533/101 , C12Q2522/101 , C12Q2549/101
摘要： Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.
摘要翻译： 提供了用于进行核酸复制和扩增反应的方法和组合物。 在反应混合物中选择并提供单链核酸结合蛋白，所述反应混合物在低，非收缩温度下组装以包括用于成功的核酸复制或扩增反应的所有必需试剂。 通过在低温下将单链核酸结合蛋白并入反应混合物中，尽管反应混合物已经在非收敛温度下完全组装，但非特异性产物如扩增产物的产生得到改善。
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公开(公告)号：US20110189736A1
公开(公告)日：2011-08-04
申请号：US13074752
申请日：2011-03-29
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2549/125 , C12Q2533/101 , C12Q2522/101 , C12Q2549/101
摘要： Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.
摘要翻译： 提供了用于进行核酸复制和扩增反应的方法和组合物。 在反应混合物中选择并提供单链核酸结合蛋白，所述反应混合物在低，非收缩温度下组装以包括用于成功的核酸复制或扩增反应的所有必需试剂。 通过在低温下将单链核酸结合蛋白并入反应混合物中，尽管反应混合物已经在非收敛温度下完全组装，但非特异性产物如扩增产物的产生得到改善。
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4.发明授权Recombinant Colwellia psychrerythraea alkaline phosphatase and uses thereof 有权重组Colwellia psychrerythraea碱性磷酸酶及其用途公开(公告)号：US08486665B2
公开(公告)日：2013-07-16
申请号：US13356268
申请日：2012-01-23
CPC分类号： C12N9/16 , C12Y301/03001
摘要： A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated from Colwellia psychrerythraea are provided. Methods of over-expression and purification of the recombinant alkaline phosphatase and mutants thereof are also disclosed. Methods of over-expressing and purifying commercially useful quantities of active recombinant heat labile alkaline phosphatase fusion enzymes from C. psychrerythraea, wherein the fusion enzymes comprise one or more heterologous leader sequences are disclosed. The disclosed C. psychrerythraea heat labile alkaline phosphatase has properties similar to shrimp alkaline phosphatase and can be substituted for shrimp alkaline phosphatase in assays involving the same.
摘要翻译： 公开了一种热不稳定碱性磷酸酶及其使用方法及其试剂盒。 具体地，提供了从Colleyia psychrerythraea分离的热不稳定碱性磷酸酶的肽序列，使用方法和试剂盒的核苷酸序列。 还公开了重组碱性磷酸酶及其突变体的过表达和纯化方法。 过度表达和纯化商业上有用量的来自苦参精油的活性重组热不稳定碱性磷酸酶融合酶的方法，其中所述融合酶包含一个或多个异源前导序列。 所披露的C. psychrerythraea热不稳定碱性磷酸酶具有类似于虾碱性磷酸酶的性质，并且可以在涉及其的测定中替代虾碱性磷酸酶。
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5.发明授权Recombinant colwellia psychrerythraea alkaline phosphatase and uses thereof 有权重组大肠杆菌psychrerythraea碱性磷酸酶及其用途公开(公告)号：US08129168B2
公开(公告)日：2012-03-06
申请号：US12687677
申请日：2010-01-14
CPC分类号： C12N9/16 , C12Y301/03001
摘要： A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated from Colwellia psychrerythraea are provided. Methods of over-expression and purification of the recombinant alkaline phosphatase and mutants thereof are also disclosed. Methods of over-expressing and purifying commercially useful quantities of active recombinant heat labile alkaline phosphatase fusion enzymes from C. psychrerythraea, wherein the fusion enzymes comprise one or more heterologous leader sequences are disclosed. The disclosed C. psychrerythraea heat labile alkaline phosphatase has properties similar to shrimp alkaline phosphatase and can be substituted for shrimp alkaline phosphatase in assays involving the same.
摘要翻译： 公开了一种热不稳定碱性磷酸酶及其使用方法及其试剂盒。 具体地，提供了从Colleyia psychrerythraea分离的热不稳定碱性磷酸酶的肽序列，使用方法和试剂盒的核苷酸序列。 还公开了重组碱性磷酸酶及其突变体的过表达和纯化方法。 过度表达和纯化商业上有用量的来自苦参精油的活性重组热不稳定碱性磷酸酶融合酶的方法，其中所述融合酶包含一个或多个异源前导序列。 所披露的C. psychrerythraea热不稳定碱性磷酸酶具有类似于虾碱性磷酸酶的性质，并且可以在涉及其的测定中替代虾碱性磷酸酶。
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