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    Everninomicin biosynthetic genes
    13.
    发明授权
    Everninomicin biosynthetic genes 失效
    Everninomicin生物合成基因

    公开(公告)号：US07947480B2

    公开(公告)日：2011-05-24

    申请号：US12875342

    申请日：2010-09-03

    申请人： Thomas J. Hosted ,  Tim X. Wang ,  Ann C. Horan

    发明人： Thomas J. Hosted ,  Tim X. Wang ,  Ann C. Horan

    IPC分类号： C12N9/02 ,  C12N9/00 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C12P19/34 ,  A61K39/395 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N9/22 ,  C12N15/52 ,  C12N15/76 ,  C12N15/90 ,  C12N2800/30 ,  C12P19/04 ,  C12P19/60

    摘要： This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A Micromonospora site-specific integrase gene is also provided, which can be incorporated in a vector for integration into any actinomycete, and, particularly into Monospora. Thus, the invention further provides methods for introducing heterologous genes into an actinomycete chromosome using this particular vector.

    摘要翻译： 本发明涉及编码引导正交霉素埃芬诺米星的合成的蛋白质的核酸，以及使用核酸和蛋白质产生基于依维诺霉素结构显示抗生素活性的化合物的核酸。 提供了负责编码everninomicin生物合成基因的基因簇的DNA序列，其提供了生产everninomicin的机制。 因此，本发明提供了合成基于everninomicin的新型everninomicin相关化合物所需的核酸序列，其由设计用于改变everninomicin中所含的糖基和修饰的orsellinic酸基团的DNA序列的修饰产生。 还提供了一个小单孢菌属位点特异性整合酶基因，其可以并入载体中以整合到任何放线菌，特别是单孢菌素中。 因此，本发明还提供了使用该特定载体将异源基因引入放线菌染色体的方法。

    2-O-(β-D-glucopyranosyl) ascorbic acid, process for its production, and foods cosmetics containing compositions comprising it
    14.
    发明授权
    2-O-(β-D-glucopyranosyl) ascorbic acid, process for its production, and foods cosmetics containing compositions comprising it 有权
    2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸，其生产方法，以及含有组合物的食品化妆品

    公开(公告)号：US07943583B2

    公开(公告)日：2011-05-17

    申请号：US11501074

    申请日：2006-08-09

    申请人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

    发明人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

    IPC分类号： A01N43/04 ,  A01N43/06

    CPC分类号： A61K8/676 ,  A23L33/105 ,  A23L33/15 ,  A23V2002/00 ,  A61K8/602 ,  A61K31/375 ,  A61K2800/52 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/02 ,  C07H1/08 ,  C07H17/04 ,  C12P19/60 ,  A23V2250/708

    摘要： The present invention provides a novel ascorbic acid derivative as a provitamin C with improved stability in the body and prolonged life in the body compared to conventionally known 2-O-(β-D-glucopyranosyl)ascorbic acid. The composition comprising the novel compound 2-O- (β-D-glucopyranosyl)ascorbic acid has been extracted from plants such as from Ningxia Lycium barbarum L. and/or Lycium chinense Mill. The compositions comprising 2-O-(βD-glucopyranosyl) ascorbic acid may be enzymatically synthesized using β-D-glucosyltransferase. Pure 2-O-(β-D-glucopyranosyl)ascorbic acid may be produced from such compositions. Alternatively, 2-O-(β-D-glucopyranosyl)ascorbic acid may be produced by chemical synthesis. The 2-O-(β-D-glucopyranosyl)ascorbic acid results in higher stability and a prolonged life of vitamin C when ingested in the body compared to the corresponding α-D-glucopyranosyl derivative, and is therefore highly suitable as a provitamin C to be used in cosmetics and foods.

    摘要翻译： 本发明提供了一种新颖的抗坏血酸衍生物作为前维生素C，与常规已知的2-O-（β-葡萄糖-D-吡喃葡萄糖基）抗坏血酸相比，体内的稳定性和体内寿命延长。 含有新化合物2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸的组合物已经从宁夏枸杞子和/或枸杞子等植物中提取出来。 包含2-O-（β-葡萄糖吡喃糖基）抗坏血酸的组合物可以使用β-葡糖基转移酶酶促合成。 可以从这样的组合物制备纯2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸。 或者，可通过化学合成制备2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸。 与对应的α-D-吡喃葡萄糖基衍生物相比，2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸在身体摄取时导致更高的稳定性和更长的维生素C的使用寿命，因此非常适合作为维生素 C用于化妆品和食品。

    DESHYDROXY VANCOMYCIN, THE PREPARATION, PHARMACEUTICAL COMPOSITION AND THE USE
    15.
    发明申请
    DESHYDROXY VANCOMYCIN, THE PREPARATION, PHARMACEUTICAL COMPOSITION AND THE USE 有权
    脱乙酰甘氨酸，制剂，药物组合物及其用途

    公开(公告)号：US20100222252A1

    公开(公告)日：2010-09-02

    申请号：US12680650

    申请日：2008-08-25

    申请人： Bingxiang Xu ,  Haisong Xie ,  Huan Yu ,  Wei Mao ,  Weidong Ye

    发明人： Bingxiang Xu ,  Haisong Xie ,  Huan Yu ,  Wei Mao ,  Weidong Ye

    IPC分类号： A61K38/14 ,  C07K9/00 ,  C07K1/22 ,  A61P31/04

    CPC分类号： C07K9/008 ,  A61K38/14 ,  C12P19/46 ,  C12P19/60

    摘要： The present invention provides a deshydroxy vancomycin compound,a method of its preparation and a pharmaceutical composition comprising a pharmaceutically effective amount of the deshydroxy vancomycin and the use of the said composition in the preparation of drugs for the treatment of susceptible bacteria infections. The method includes the following steps: (1) preparing a concentrated vancomycin solution containing the deshydroxy vancomycin by fermentations of Amycolatopsis Orientalis with Deposit No. CGMCCNO.1183; (2) separating and purifing the concentrated vancomycin solution to obtain a refined filtrate of vancomycin hydrochloride containing the deshydroxy vancomycin by column chromatography; and (3) further separating and purifing the refined filtrate to obtain the deshydroxy vancomycin by chromatography. Wherein, separation and purification is processed by column chromatography in a gel chromatography column containing a salt-water mobile phase, separation and purification is processed by chromatography in a macroporous adsorption resin chromatography column containing a buffer-methanol mobile phase.

    摘要翻译： 本发明提供了一种脱羟基万古霉素化合物，其制备方法和包含药学有效量的脱羟万古霉素的药物组合物，以及所述组合物在制备用于治疗易感细菌感染的药物中的用途。 该方法包括以下步骤：（1）通过发酵具有沉积物号CGMCCNO.1183的Amycolatopsis Orientalis的发酵制备含有脱羟万古霉素的浓缩万古霉素溶液; （2）分离纯化浓缩的万古霉素溶液，通过柱色谱法得到含有脱羟万古霉素的万古霉素盐酸盐精制滤液; 和（3）进一步分离和纯化精制滤液，通过色谱法获得脱羟万古霉素。 其中分离纯化在含有盐水流动相的凝胶色谱柱中通过柱色谱法处理，分离和纯化在含有缓冲液 - 甲醇流动相的大孔吸附树脂色谱柱中通过色谱法处理。

    Method for manufacturing α-glycosylisoquercitrin, intermediate product and by-product thereof
    16.
    发明授权
    Method for manufacturing α-glycosylisoquercitrin, intermediate product and by-product thereof 有权
    α-糖基异槲皮素的制备方法，其中间产物及其副产物

    公开(公告)号：US07691425B2

    公开(公告)日：2010-04-06

    申请号：US10740607

    申请日：2003-12-22

    申请人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    发明人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    IPC分类号： A23L1/00

    CPC分类号： C12P19/60 ,  A23L5/41 ,  A23L33/105 ,  A61K31/352 ,  A61K31/7048 ,  C12P17/06

    摘要： The present invention provides a method for producing isoquercitrin, α-glycosylisoquercitrin, and rhamnose, the method comprising a step of naringin-degrading enzyme treatment during the isoquercitrin production from rutin in the presence of an edible component, such as gelatin, wheat gluten, chitosan, lecithin, a glycerol fatty acid ester, xanthan gum, carrageenan, sodium chondroitin sulfate, casein, enzymatically decomposed gelatin, sodium alginate, konjac extract, gellan gum, guar gum, soybean protein, agar, pectin, yeast extract, egg-white peptide, cluster dextrin, gum arabic, arginine, sodium metaphosphate, karaya gum, locust bean gum, sodium pyrophosphate, glucosamine, chitin, sodium glutamate, dextrin, trehalose, or a grain-based food ingredients. According to the present invention, isoquercitrin and α-glycosylisoquercitrin, which are of use as antioxidants, anti-fading agents, flavor change inhibitors, etc., can be produced in enhanced yields.

    摘要翻译： 本发明提供异槲皮素，α-糖基异槲皮素和鼠李糖的制造方法，其特征在于，在可食用成分如明胶，小麦面筋，壳聚糖等存在下，由芦丁生产异槲皮素期间柚皮苷降解酶处理步骤 ，卵磷脂，甘油脂肪酸酯，黄原胶，角叉菜胶，硫酸软骨素钠，酪蛋白，酶分解明胶，藻酸钠，魔芋提取物，结冷胶，瓜尔胶，大豆蛋白，琼脂，果胶，酵母提取物，蛋白肽 ，糊精，阿拉伯树胶，精氨酸，偏磷酸钠，刺槐豆胶，刺槐豆胶，焦磷酸钠，葡糖胺，甲壳素，谷氨酸钠，糊精，海藻糖或谷物类食品成分。 根据本发明，可以以增加的产率生产用作抗氧化剂的异槲皮素和α-糖基异槲皮素，抗褪色剂，风味变化抑制剂等。

    Water Soluble and Activable Phenolics Derivatives with Dermocosmetic and Therapeutic Applications and Process for Preparing Said Derivatives
    17.
    发明申请
    Water Soluble and Activable Phenolics Derivatives with Dermocosmetic and Therapeutic Applications and Process for Preparing Said Derivatives 审中-公开
    具有皮肤科学和治疗应用的水溶性和活性酚类衍生物以及用于制备所述衍生物的方法

    公开(公告)号：US20090233876A1

    公开(公告)日：2009-09-17

    申请号：US12304212

    申请日：2007-06-13

    申请人： Daniel Auriol ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

    发明人： Daniel Auriol ,  Renaud Nalin ,  Patrick Robe ,  Fabrice Lefevre

    IPC分类号： A61K31/7048 ,  C12P19/60 ,  C07H17/06 ,  A61P35/00

    CPC分类号： C07H17/075 ,  C07H15/203 ,  C07H15/24 ,  C07H15/26 ,  C12P17/02 ,  C12P19/02 ,  C12P19/44 ,  C12P19/60

    摘要： The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-α-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrhythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic antimutagenic, antineoplastic, anti-thrombotic, and vasodilatory formulations, or in any other field of application.

    摘要翻译： 本发明涉及通过将选自邻苯二酚或其衍生物的酚类与蔗糖的葡萄糖部分的酶缩合制备酚衍生物。 所述酚衍生物的生产通过葡糖基转移酶（EC 2.4.1.5）来实现。 所选择的酚类的这些O-α-葡萄糖苷是新的，在水中的溶解度高于其亲本多酚的溶解度，并且在化妆品和药物组合物中有用，例如抗氧化，抗病毒，抗菌，免疫刺激，抗过敏，抗高血压， 抗缺血性，抗心律不齐，抗血栓形成，低胆固醇血症，抗脂质过氧化物，肝保护，抗炎，抗致癌性抗诱发剂，抗肿瘤剂，抗血栓形成和血管扩张制剂，或在任何其他应用领域。

    Quercetin Glycoside Composition and Method of Preparing the Same
    18.
    发明申请
    Quercetin Glycoside Composition and Method of Preparing the Same 审中-公开
    槲皮素糖苷组合物及其制备方法

    公开(公告)号：US20090143317A1

    公开(公告)日：2009-06-04

    申请号：US11794218

    申请日：2005-12-28

    申请人： Yoshiko Ono ,  Namino Tomimori ,  Norifumi Tateishi ,  Masamitsu Moriwaki ,  Kazuhiro Emura ,  Shuji Okuyama

    发明人： Yoshiko Ono ,  Namino Tomimori ,  Norifumi Tateishi ,  Masamitsu Moriwaki ,  Kazuhiro Emura ,  Shuji Okuyama

    IPC分类号： A61K31/7048 ,  C12P19/60

    CPC分类号： C12P19/22 ,  A23L3/3463 ,  A23L3/3562 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/7048 ,  C12P19/60 ,  A61K2300/00 ,  A23V2200/02 ,  A23V2250/21168

    摘要： The present invention provides an α-glycosyl isoquercitrin-containing novel composition which has a high in vivo absorbability, and hence exhibits a significant in vivo antioxidative activity. The present invention further provides preparation methods for such a composition. The composition contains a mixture of quercetin glycosides represented by the following formula: wherein Glc represents a glucose residue, and n is 0 or a positive integer of 1 or more,includes at least a quercetin glycoside wherein n is 3, and satisfies the following requirement (a):(a) the total proportion of quercetin glycosides in which n is 3, and in which other n values may be 1 or 2, or 1 and 2, is 50 mol % or more, and the total proportion of quercetin glycosides wherein n is 4 or more is 15 mol % or less, in the composition. The composition can be prepared by treating an enzymatically modified isoquercitrin with β-amylase.

    摘要翻译： 本发明提供了具有高体内吸收性的α-糖基异槲皮苷新化合物，因此具有显着的体内抗氧化活性。 本发明还提供了这种组合物的制备方法。 该组合物含有由下式表示的槲皮素糖苷的混合物：其中，Glc表示葡萄糖残基，n为0或1以上的正整数，至少包含槲皮素苷，n为3，满足以下要求 （a）：（a）其中n为3，其它n值可以为1或2，或1和2的槲皮素苷的总比例为50摩尔％以上，槲皮素苷的总比例 n为4以上15摩尔％以下。 可以通过用β-淀粉酶处理酶促修饰的异槲皮素来制备组合物。