公开(公告)号：US20240247249A1

公开(公告)日：2024-07-25

申请号：US18491943

申请日：2023-10-23

Applicant: CureVac Manufacturing GmbH

Inventor： Andreas FUNKNER ,  Stefanie DORNER ,  Stefanie SEWING ,  Johannes KAMM ,  Norbert BROGHAMMER ,  Thomas KETTERER ,  Thorsten MUTZKE

IPC: C12N15/10 ,  B01D61/14 ,  B01D71/12 ,  C12P19/34 ,  G01N30/02

CPC classification number: C12N15/1017 ,  B01D61/145 ,  B01D71/12 ,  C12P19/34 ,  G01N30/02 ,  G01N2030/027

Abstract: The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA: transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

公开(公告)号：US20240247246A1

公开(公告)日：2024-07-25

申请号：US18418226

申请日：2024-01-20

Applicant: AbClonal Science, Inc.

Inventor： Dapeng Sun ,  Zhenyu Zhu ,  Aine Quimby

IPC: C12N9/00 ,  C12P19/34

CPC classification number: C12N9/93 ,  C12P19/34 ,  C12Y605/01001

Abstract: The invention includes a mutant T7 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T7 DNA ligase, on a blunt-ended dsDNA substrate. The mutant T7 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, which are E63K (SEQ ID NO:3; SEQ ID NO:4), D132R (SEQ ID NO:5; SEQ ID NO:6), E243K (SEQ ID NO:7; SEQ ID NO: 8), D245R (SEQ ID NO:9; SEQ ID NO: 10), E272K (SEQ ID NO:11; SEQ ID NO: 12), D288R (SEQ ID NO:13; SEQ ID NO:14), E289K (SEQ ID NO:15; SEQ ID NO: 16), and E292K (SEQ ID NO:17; SEQ ID NO:18).

公开(公告)号：US20240240218A1

公开(公告)日：2024-07-18

申请号：US18561922

申请日：2022-05-18

Applicant: Flagship Pioneering Innovations VI, LLC

Inventor： Alexandra Sophie De Boer ,  Elissa Magdalene HOBERT ,  Joseph Arthur DEPETER

IPC: C12P19/34

CPC classification number: C12P19/34

Abstract: The methods of the disclosure can be used to enrich a population of circular polyribonucleotides in a mixture of linear polyribonucleotides and circular polyribonucleotides.

公开(公告)号：US20240240217A1

公开(公告)日：2024-07-18

申请号：US18530865

申请日：2023-12-06

Applicant: Illumina, Inc.

Inventor： Jean-Alexandre Richard ,  Xiangyuan Yang ,  Daniel Hartoyo Lukamto ,  Ramesh Neelakandan

IPC: C12P19/34 ,  C07H15/26 ,  C07H19/048 ,  C12Q1/6869 ,  G01N21/64

CPC classification number: C12P19/34 ,  C07H15/26 ,  C07H19/048 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6439

Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3′ vinyl or isonitrile containing blocking groups and/or tetrazine or strained unsaturated ring containing cleavable linkers. Additionally, the present disclosure provides methods of using the nucleoside/nucleotide in oligonucleotide synthesis, and methods of sequencing using the nucleotide described herein.

公开(公告)号：US20240218412A1

公开(公告)日：2024-07-04

申请号：US18582620

申请日：2024-02-20

Applicant: CureVac Manufacturing GmbH

Inventor： Aniela WOCHNER ,  Tilmann ROOS ,  Thomas KETTERER

IPC: C12P19/34 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/40 ,  C12M3/00 ,  C12Q1/6844

CPC classification number: C12P19/34 ,  C12M21/18 ,  C12M23/44 ,  C12M29/04 ,  C12M29/14 ,  C12M29/18 ,  C12M41/26 ,  C12M41/32 ,  C12M41/48 ,  C12Q1/6844

Abstract: The present invention relates to a method for synthesizing an RNA molecule of a given sequence, comprising the step of determining the fraction (1) for each of the four nucleotides G, A, C and U in said RNA molecule, and the step of synthesizing said RNA molecule by in vitro transcription in a sequence-optimized reaction mix, wherein said sequence-optimized reaction mix comprises the four ribonucleoside triphosphates GTP, ATP, CTP and UTP, wherein the fraction (2) of each of the four ribonucleoside triphosphates in the sequence-optimized reaction mix corresponds to the fraction (1) of the respective nucleotide in said RNA molecule, a buffer, a DNA template, and an RNA polymerase.
Further, the present invention relates to a bioreactor (1) for synthesizing RNA molecules of a given sequence, the bioreactor (1) having a reaction module (2) for carrying out in vitro RNA transcription reactions in a sequence-optimized reaction mix, a capture module (3) for temporarily capturing the transcribed RNA molecules, and a control module (4) for controlling the infeed of components of the sequence-optimized reaction mix into the reaction module (2), wherein the reaction module (2) comprises a filtration membrane (21) for separating nucleotides from the reaction mix, and the control of the infeed of components of the sequence-optimized reaction mix by the control module (4) is based on a measured concentration of separated nucleotides.

公开(公告)号：US20240218382A1

公开(公告)日：2024-07-04

申请号：US18533474

申请日：2023-12-08

Applicant: ALDEVRON, L.L.C.

Inventor： James A. WILLIAMS

IPC: C12N15/79 ,  A61K48/00 ,  C12N15/63 ,  C12N15/64 ,  C12N15/67 ,  C12N15/85 ,  C12P19/34 ,  C12P21/00

CPC classification number: C12N15/79 ,  C12N15/63 ,  C12N15/64 ,  C12N15/67 ,  C12N15/85 ,  C12P19/34 ,  C12P21/00 ,  A61K48/00 ,  C12N2800/107 ,  C12N2800/24 ,  C12N2820/55 ,  C12N2830/42

Abstract: A eukaryotic replicative pUC-free minicircle expression vector is provided. The eukaryotic replicative pUC-free minicircle expression vector includes a pUC-free eukaryotic region sequence encoding a transgene of interest and comprising 5′ and 3′ ends and a ii) pUC-free spacer region of less than 500 basepairs in length linking the 5′ and 3′ ends of the eukaryotic region sequences and comprising a bacterial R6K replication origin having at least 95% sequence identity to SEQ ID NO: 11 and SEQ ID NO: 12 and a RNA selectable marker, the RNA selectable marker being an RNA-IN regulating RNA-OUT functional variant having at least 95% sequence identity to SEQ ID NO: 20 or SEQ ID NO: 22.

公开(公告)号：US20240209407A1

公开(公告)日：2024-06-27

申请号：US18556773

申请日：2022-04-26

Applicant: CAMENA BIOSCIENCE LIMITED

Inventor： Derek STEMPLE ,  Sylwia MANKOWSKA ,  Neil BELL

IPC: C12P19/34 ,  C12N9/00 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12P19/34 ,  C12N9/22 ,  C12N9/93 ,  C12N15/11 ,  C12Y605/01001 ,  C12N2310/16 ,  C12N2310/531

Abstract: Described herein are compositions of matter and methods to synthesize any nucleic acid (NA) sequence using completely natural nucleic acid sources without the need for large-scale phosphoramidite-mediated chemical synthesis.

公开(公告)号：US12012594B2

公开(公告)日：2024-06-18

申请号：US17721195

申请日：2022-04-14

Applicant: The Regents of the University of California

Inventor： Daniel S. Rokhsar

IPC: C40B40/06 ,  B01J19/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6869 ,  C40B50/00 ,  G16B50/00 ,  G16B50/30

CPC classification number: C12N15/1065 ,  B01J19/0046 ,  C12N15/1006 ,  C12P19/34 ,  C12Q1/6869 ,  C40B40/06 ,  C40B50/00 ,  G16B50/00 ,  G16B50/30 ,  C12Q2525/191 ,  C12Q2563/185 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2563/185

Abstract: Various approaches for generating read-sets from nucleic acid molecules and segments and phasing are disclosed. Nucleic acids are assembled into complexes using binding moieties and exposed nucleic acid ends are tagged with nucleic acid tags. Read-sets can be generated from tagged nucleic acid molecules and segments. Physical linkage relationships between nucleic acid molecules and segments can be examined using the nucleic acid tags. Various approaches to generating read-sets and phasing are presented.

公开(公告)号：US11999978B2

公开(公告)日：2024-06-04

申请号：US17279686

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC: C12N9/16 ,  C12P19/34

CPC classification number: C12N9/16 ,  C12P19/34 ,  C12Y301/03001

Abstract: A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X1/Y)×100≤0.6000 (1); (X2/Y)×100≤0.1800 (2); (X3/Y)×100≤0.2000 (3); (X4/Y)×100≤0.8000 (4); (X5/Y)×100≤1.6000 (5); and (X6/Y)×100≤0.3500 (6), wherein X1 to X6 represent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

公开(公告)号：US11993801B2

公开(公告)日：2024-05-28

申请号：US17381024

申请日：2021-07-20

Applicant: Illumina Singapore Pte. Ltd. ,  Nanyang Technological University

Inventor： Pin Koon Ee ,  Yin Nah Teo ,  Shunsuke Chiba

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1264 ,  C12Y207/07031

Abstract: Disclosed herein include methods and compositions for nucleic acid synthesis using a terminal deoxynucleotidyl transferase with deoxyribonucleotide trisphosphates each comprising a modified base with a photocleavable carbon chain moiety that enables single incorporations when present.