公开(公告)号：US20210171925A1

公开(公告)日：2021-06-10

申请号：US17171017

申请日：2021-02-09

Applicant: ABclonal Science, Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun ,  Aine Quimby ,  Michaela Shottes

IPC: C12N9/22 ,  C12Q1/37

Abstract: Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).

公开(公告)号：US20240052326A1

公开(公告)日：2024-02-15

申请号：US18130012

申请日：2023-04-03

Applicant: AbClonal Science, Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun ,  Aine Quimby

IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6853

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Y207/07007

Abstract: Disclosed are Taq DNA polymerase mutants which exhibit enhanced efficiency in qPCR compared to the wild type Taq DNA polymerase. Such mutants include: V62S, V64S, A70F, F73A, A77F, P253G, E255K, D257R, A259F, A271F, L288S, E289K, S357I, L361S, L376S, P382G, T385I, G418P, R419D, E421K, L461S, A472F, E497K, L498S, E524K, D551R, R556D, S679l, L789S, E189K/E507K/E742K (See Sequence Listing Guide for the mutants' amino acid and protein sequences).

公开(公告)号：US11821010B2

公开(公告)日：2023-11-21

申请号：US17106804

申请日：2020-11-30

Applicant: AbClonal Science, Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun

IPC: C12N9/12 ,  C12N15/63 ,  C12Q1/686 ,  C12Q1/6848

CPC classification number: C12N9/1252 ,  C12N9/12 ,  C12N15/63 ,  C12Q1/686 ,  C12Q2521/10 ,  C12Q2527/101 ,  C12Q2533/101

Abstract: The invention includes a mutant Taq polymerase, which can significantly extend and amplify a target sequence where the extension conditions are time limited to as little as one second. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

公开(公告)号：US20230332117A9

公开(公告)日：2023-10-19

申请号：US17487643

申请日：2021-09-28

Applicant: AbClonal Science Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

公开(公告)号：US11685913B1

公开(公告)日：2023-06-27

申请号：US17675278

申请日：2022-02-18

Applicant: AbClonal Science, Inc.

Inventor： Alicia DiCicco ,  Zhenyu Zhu ,  Aine Quimby

IPC: C12N9/00 ,  C12Q1/6869

CPC classification number: C12N9/93 ,  C12Q1/6869 ,  C12Y605/01001

Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5′ and 3′ ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.

公开(公告)号：US11168312B2

公开(公告)日：2021-11-09

申请号：US16813488

申请日：2020-03-09

Applicant: AbClonal Science Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun ,  Weiling Peng

IPC: C12N9/12 ,  C12Q1/686 ,  C12N15/63

Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

公开(公告)号：US20240392336A1

公开(公告)日：2024-11-28

申请号：US18418223

申请日：2024-01-20

Applicant: AbClonal Science, Inc.

Inventor： Dapeng Sun ,  Zhenyu Zhu ,  Aine Quimby

IPC: C12P19/34 ,  C12N9/00

Abstract: Disclosed is a novel T7 DNA ligase mutant with the mutation R129D, that had higher specificity toward cohesive end-ligation even in the presence of polyethylene glycol. Controlling the composition of final ligation products is important and thus, a variant which improves the specificity of T7 DNA ligase to cohesive ends will generate more reliable results.

公开(公告)号：US11767524B2

公开(公告)日：2023-09-26

申请号：US17027747

申请日：2020-09-22

Applicant: ABclonal Science Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun ,  Michaela Shottes

IPC: C12N15/10 ,  C12N9/22

CPC classification number: C12N15/1013 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1065 ,  C07K2319/00

Abstract: Disclosed is a product and process, wherein one adds a N-terminal Histamine-Maltose Binding Protein (“MBP”) tag to endonucleases, including restriction endonucleases like Hind III, and binds the tagged fusion protein to a solid support, preferably beads, once the enzyme has digested oligonucleotides in solution, in order to arrest further digestion. Preferred beads for binding the tagged enzyme are magnetic beads, which can easily be removed from solution by binding to a support and then removing it, or can be accumulated by magnetic attraction in a particular region. More preferred are magnetic beads bound to iminodiacetic acid or nitrilotriacetic acid.

公开(公告)号：US20230094503A1

公开(公告)日：2023-03-30

申请号：US17487643

申请日：2021-09-28

Applicant: AbClonal Science Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun

IPC: C12N9/12

Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

公开(公告)号：US20220106576A1

公开(公告)日：2022-04-07

申请号：US17470557

申请日：2021-09-09

Applicant: AbClonal Science, Inc.

Inventor： Zhenyu Zhu ,  Dapeng Sun ,  Aine Quimby

IPC: C12N9/12 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Disclosed are Taq DNA polymerase mutants which exhibit enhanced efficiency in qPCR compared to the wild type Taq DNA polymerase. Such mutants include: V62S, V64S, A70F, F73A, A77F, P253G, E255K, D257R, A259F, A271F, L288S, E289K, S357I, L361S, L376S, P382G, T385I, G418P, R419D, E421K, L461S, A472F, E497K, L498S, E524K, D551R, R556D, S679I, L789S, E189K/E507K/E742K (See Sequence Listing Guide for the mutants' amino acid and protein sequences).