公开(公告)号：US20210395708A1

公开(公告)日：2021-12-23

申请号：US17279686

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC: C12N9/16 ,  C12P19/34

Abstract: A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X1/Y)×100≤0.6000 (1); (X2/Y)×100≤0.1800 (2); (X3/Y)×100≤0.2000 (3); (X4/Y)×100≤0.8000 (4); (X5/Y)×100≤1.6000 (5); and (X6/Y)×100≤0.3500 (6), wherein X1 to X6 represent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

公开(公告)号：US20210332337A1

公开(公告)日：2021-10-28

申请号：US17279800

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Yoji Ueda ,  Yuki Takii ,  Masateru Ito ,  Mai Yagi

IPC: C12N9/16 ,  C12Q1/6806

Abstract: A composition contains an alkaline phosphatase; and a peptide fragment group (A) composed of two or more peptide fragments, wherein each of the two or more peptide fragments consists of 5 to 50 consecutive amino acid residues selected from positions 501 to 578 of the amino acid sequence set forth in SEQ ID NO: 5, wherein a content ratio of the peptide fragment group (A) to the alkaline phosphatase satisfies formula (A): (XA/Y)×100≤4.4000 (A), wherein XA represents a peak area value of the peptide fragment group (A) calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

公开(公告)号：US11999978B2

公开(公告)日：2024-06-04

申请号：US17279686

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC: C12N9/16 ,  C12P19/34

CPC classification number: C12N9/16 ,  C12P19/34 ,  C12Y301/03001

Abstract: A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X1/Y)×100≤0.6000 (1); (X2/Y)×100≤0.1800 (2); (X3/Y)×100≤0.2000 (3); (X4/Y)×100≤0.8000 (4); (X5/Y)×100≤1.6000 (5); and (X6/Y)×100≤0.3500 (6), wherein X1 to X6 represent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

公开(公告)号：US20220033874A1

公开(公告)日：2022-02-03

申请号：US17280056

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC: C12Q1/42 ,  C12N9/16 ,  G01N30/86 ,  G01N30/72 ,  G01N30/74 ,  C12Q1/6816 ,  G01N33/68

Abstract: A method evaluates a quality of a dephosphorylation reagent, the method including the steps of: providing a dephosphorylation reagent containing an alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and evaluating the dephosphorylation reagent as having a high quality if a content ratio of the peptide fragment to the alkaline phosphatase is a predetermined reference value or less.

公开(公告)号：US12227787B2

公开(公告)日：2025-02-18

申请号：US17280056

申请日：2019-09-25

Applicant: Toray Industries, Inc.

Inventor： Masateru Ito ,  Yoji Ueda ,  Yuki Takii ,  Mai Yagi

IPC: C12Q1/42 ,  C12N9/16 ,  C12Q1/6816 ,  G01N30/72 ,  G01N30/74 ,  G01N30/86 ,  G01N33/68 ,  G01N30/02

Abstract: A method evaluates a quality of a dephosphorylation reagent, the method including the steps of: providing a dephosphorylation reagent containing an alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and evaluating the dephosphorylation reagent as having a high quality if a content ratio of the peptide fragment to the alkaline phosphatase is a predetermined reference value or less.