公开(公告)号：US20230358678A1

公开(公告)日：2023-11-09

申请号：US18339185

申请日：2023-06-21

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkeslstien ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  H01L27/146 ,  H01L27/00 ,  H01L27/088 ,  B01L3/00 ,  G01N33/543 ,  G01N27/414 ,  C12Q1/6874 ,  G01N21/05

CPC classification number: G01N21/645 ,  G01N21/6454 ,  H01L27/14623 ,  H01L27/00 ,  H01L27/088 ,  B01L3/502707 ,  G01N33/54373 ,  G01N21/6452 ,  G01N27/4145 ,  G01N21/6428 ,  B01L3/502715 ,  G01N2021/6482 ,  C12Q1/6874 ,  G01N2021/6441 ,  G01N21/05 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11786898B2

公开(公告)日：2023-10-17

申请号：US16818953

申请日：2020-03-13

Applicant: Illumina, Inc.

Inventor： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00 ,  G01N35/08 ,  C12Q1/6869

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L3/502715 ,  B01L7/52 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  G01N35/08 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

公开(公告)号：US20230184711A1

公开(公告)日：2023-06-15

申请号：US18003493

申请日：2021-06-18

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Rico Otto ,  Jeffrey G. Mandell

IPC: G01N27/414

CPC classification number: G01N27/4146

Abstract: Devices and methods of using the devices are disclosed which can provide scalability, improved sensitivity and reduced noise for sequencing polynucleotide. Examples of the devices include a biological or solid-state nanopore, a field effect transistor (FET) sensor with improved gate controllability over the channel, and a porous structure.

公开(公告)号：US20230145389A1

公开(公告)日：2023-05-11

申请号：US18087499

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  F16K99/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502738 ,  B01L7/525 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/065 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0074 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US11629373B2

公开(公告)日：2023-04-18

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20230112203A1

公开(公告)日：2023-04-13

申请号：US17934175

申请日：2022-09-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Boyan Boyanov ,  Craig Ciesla ,  Anthony Flannery ,  Gerald Kreindl ,  Rean Silke Musa ,  Darren Segale

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/414 ,  B01L3/00

Abstract: Devices for sequencing biopolymers and methods of using the devices are disclosed. In one example, such a device has a nanopore, a plurality of wells and fluidic tunnels to allow a biopolymer to translocate in the device. In some embodiments, the device may include integrated electronics or micro-electromechanical systems, such as valves, bubble generators/annihilators or pressure pulse generators, to actively control fluidic/ionic/electric flows in the device.

公开(公告)号：US11384392B2

公开(公告)日：2022-07-12

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210024991A1

公开(公告)日：2021-01-28

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  G03F7/075 ,  C08G77/04 ,  C08F220/56

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US10605766B2

公开(公告)日：2020-03-31

申请号：US14798762

申请日：2015-07-14

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Jeffrey G. Mandell ,  Jingwei Bai ,  Kevin L. Gunderson ,  Cheng-Yao Chen ,  Michel Perbost

IPC: C12Q1/68 ,  G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825 ,  B82Y15/00

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US10551342B2

公开(公告)日：2020-02-04

申请号：US16024299

申请日：2018-06-29

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov

IPC: H01L21/02 ,  G01N27/414 ,  C12Q1/6869 ,  G01R19/00 ,  H03K3/03

Abstract: Apparatus and methods are disclosed for single molecule field effect sensors having conductive channels functionalized with a single active moiety. A region of a nanostructure (e.g., such as a silicon nanowire or a carbon nanotube) provide the conductive channel. Trapped state density of the nanostructure is modified for a portion of the nanostructure in proximity with a location where the active moiety is linked to the nanostructure. In one example, the semiconductor device includes a source, a drain, a channel including a nanostructure having a modified portion with an increased trap state density, the modified portion being further functionalized with an active moiety. A gate terminal is in electrical communication with the nanostructure. As a varying electrical signal is applied to an ionic solution in contact with the nanostructure channel, changes in current observed from the semiconductor device can be used to identify composition of the analyte.