公开(公告)号：US20130210080A1

公开(公告)日：2013-08-15

申请号：US13763352

申请日：2013-02-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya RAJAGOPAL ,  Samson CHEN ,  Axel SCHERER ,  Christopher I. WALKER

IPC: B01L7/00 ,  H05K3/46

CPC classification number: B01L7/52 ,  B01L3/502707 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0893 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/686 ,  H05K1/0212 ,  H05K1/0272 ,  H05K1/028 ,  H05K3/46 ,  H05K3/4697 ,  Y10T29/49124

Abstract: An apparatus for performing a Polymerase Chain Reaction (PCR) is disclosed. The apparatus comprises a PCR chamber for performing a Polymerase Chain Reaction and a printed circuit board (PCB) fluidic device. The PCR chamber is a fluidic chamber and is located in, or is part of, the printed circuit board (PCB) fluidic device. A method for manufacturing an apparatus for performing the Polymerase Chain Reaction and a method for performing the Polymerase Chain Reaction are further disclosed.

Abstract translation: 公开了一种用于进行聚合酶链式反应（PCR）的装置。 该装置包括用于进行聚合酶链式反应的PCR室和印刷电路板（PCB）流体装置。 PCR室是流体室，并且位于印刷电路板（PCB）流体装置中或者是其中的一部分。 进一步公开了进行聚合酶链式反应的装置的制造方法和进行聚合酶链式反应的方法。

公开(公告)号：US20240018573A1

公开(公告)日：2024-01-18

申请号：US18352112

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/6486 ,  G06F17/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20150222073A1

公开(公告)日：2015-08-06

申请号：US14684280

申请日：2015-04-10

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Seheon KIM ,  Axel SCHERER ,  Aditya RAJAGOPAL ,  Chieh-Feng CHANG

IPC: H01S3/06 ,  H01S3/091 ,  H01S3/02 ,  H01S3/115 ,  H01S5/30

CPC classification number: H01S3/0627 ,  A61B5/1459 ,  H01S3/025 ,  H01S3/091 ,  H01S3/102 ,  H01S3/1028 ,  H01S3/105 ,  H01S3/115 ,  H01S5/0607 ,  H01S5/0612 ,  H01S5/1042 ,  H01S5/3013

Abstract: A microlaser system includes an optical source, a microlaser, an actuator switch, and a photovoltaic power source. The microlaser, which includes a control element, is optically pumped by at least a portion of light emitted by the optical source. The actuator switch is configured to be activated by a triggering event. Furthermore, the photovoltaic power source is coupled in a series connection with the actuator switch and the control element, the series connection configured to connect the photovoltaic power source to the control element of the microlaser when the actuator switch is activated by the triggering event.

Abstract translation: 微型激光系统包括光源，微激光器，致动器开关和光伏电源。 包括控制元件的微激光器被由光源发射的光的至少一部分光泵浦。 执行器开关被配置为由触发事件激活。 此外，光伏电源与致动器开关和控制元件串联连接，串联连接被配置为当致动器开关被触发事件激活时将光伏电源连接到微型激光器的控制元件。

公开(公告)号：US20150167092A1

公开(公告)日：2015-06-18

申请号：US14633094

申请日：2015-02-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER ,  Mark D. GOLDBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

Abstract translation: 描述了基于FRET的分析物检测和相关方法和系统，其中使用一对FRET标记的引物和/或寡核苷酸，其特异于靶向序列，其位于与FRET上呈现的FRET发色团的Förster距离的四倍 在一个或多个多核苷酸分析物中相对于另一个标记的引物和/或寡核苷酸; 特别地，一对FRET标记的引物和/或寡核苷酸与样品组合，并在测量来自至少一种FRET生色团的FRET信号之前进行一个或多个多核苷酸扩增反应。

公开(公告)号：US20140357974A1

公开(公告)日：2014-12-04

申请号：US14463392

申请日：2014-08-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya RAJAGOPAL ,  Axel SCHERER ,  Michael D. HENRY ,  Sameer WALAVALKAR ,  Thomas A. TOMBRELLO ,  Andrew P. HOMYK

IPC: H01L29/423 ,  A61B5/04 ,  H01L29/49 ,  H01L29/78 ,  H01L29/41

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: A field effect nano-pillar transistor has a pillar shaped gate element incorporating a biomimitec portion that provides various advantages over prior art devices. The small size of the nano-pillar transistor allows for advantageous insertion into cellular membranes, and the biomimitec character of the gate element operates as an advantageous interface for sensing small amplitude voltages such as transmembrane cell potentials. The nano-pillar transistor can be used in various embodiments to stimulate cells, to measure cell response, or to perform a combination of both actions.

公开(公告)号：US20140221799A1

公开(公告)日：2014-08-07

申请号：US14153819

申请日：2014-01-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya RAJAGOPAL ,  Axel SCHERER ,  Akram Sarwat SADEK

IPC: A61B5/1459 ,  A61B5/00

CPC classification number: A61B5/1459 ,  A61B5/0031 ,  A61B5/14552 ,  A61B5/6867 ,  A61B2560/0219

Abstract: A process is described for testing a biomedical property of an internal tissue of a patient. Optical energy emitted by an external source is transferred through a nail of the patient to an instrument device implanted beneath the nail. A portion of the transferred optical energy is converted to electrical power for driving components of the implanted instrument. Using the electrical power, a characteristic of the internal tissue associated with the measurement of the biomedical property is sensed and an optical signal based on the sensed characteristic is transmitted through the nail to an external data reader.

Abstract translation: 描述了用于测试患者内部组织的生物医学性质的方法。 由外部源发出的光能通过患者的指甲转移到植入在指甲下方的器械装置。 转移的光能的一部分被转换成用于驱动植入的仪器的部件的电力。 使用电力，检测与生物医学性质的测量相关联的内部组织的特性，并且基于感测到的特性的光学信号通过指甲传输到外部数据读取器。

公开(公告)号：US20140213471A1

公开(公告)日：2014-07-31

申请号：US14162725

申请日：2014-01-23

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Mark D. GOLDBERG ,  Erika F. GARCIA ,  Xiomara L. MADERO ,  Thomas A. TOMBRELLO ,  Axel SCHERER

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.

Abstract translation: 本公开提供了用于检测样品中多种分析物的方法，组合物和试剂盒。 在一些实例中，本公开提供了用于检测分析物，遗传变异，监测反应过程以及通过测量信号监测分析物 - 分析物相互作用的方法，组合物和试剂盒。 在一些示例中，可以使用信号的存在或信号的改变来构建可用于检测分析物的信号谱。

公开(公告)号：US20140134819A1

公开(公告)日：2014-05-15

申请号：US13941240

申请日：2013-07-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Chieh-Feng CHANG ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: H01L29/66

CPC classification number: H01L29/66477 ,  H01L29/0673 ,  H01L29/4232

Abstract: Methods for fabrication of nanopillar field effect transistors are described. These transistors can have high height-to-width aspect ratios and be CMOS compatible. Silicon nitride may be used as a masking material. These transistors have a variety of applications, for example they can be used for molecular sensing if the nanopillar has a functionalized layer contacted to the gate electrode. The functional layer can bind molecules, causing an electrical signal in the transistor.

Abstract translation: 描述了纳米柱场效应晶体管的制造方法。 这些晶体管可以具有高的高宽比和CMOS兼容性。 氮化硅可以用作掩模材料。 这些晶体管具有各种应用，例如，如果纳米柱具有与栅电极接触的功能化层，则它们可用于分子感测。 功能层可以结合分子，在晶体管中引起电信号。

公开(公告)号：US20140070286A1

公开(公告)日：2014-03-13

申请号：US13852480

申请日：2013-03-28

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Axel SCHERER ,  Michael D. HENRY ,  Sameer WALAVALKAR ,  Thomas A. TOMBRELLO ,  Andrew P. HOMYK

IPC: H01L29/78 ,  H01L29/66

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: A field effect nano-pillar transistor has a pillar shaped gate element incorporating a biomimitec portion that provides various advantages over prior art devices. The small size of the nano-pillar transistor allows for advantageous insertion into cellular membranes, and the biomimitec character of the gate element operates as an advantageous interface for sensing small amplitude voltages such as transmembrane cell potentials. The nano-pillar transistor can be used in various embodiments to stimulate cells, to measure cell response, or to perform a combination of both actions.

Abstract translation: 场效应纳米柱晶体管具有结合有生物医学部分的柱形门元件，其提供了优于现有技术器件的各种优点。 纳米柱晶体管的小尺寸允许有利地插入到细胞膜中，并且栅极元件的生物学特性作为用于感测小幅度电压（例如跨膜电池电位）的有利接口。 纳米柱晶体管可用于各种实施例中以刺激细胞，测量细胞反应或执行两种动作的组合。

公开(公告)号：US20130230063A1

公开(公告)日：2013-09-05

申请号：US13764620

申请日：2013-02-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Seheon KIM ,  Axel SCHERER ,  Aditya RAJAGOPAL ,  Chieh-Feng CHANG

IPC: H01S3/06

CPC classification number: H01S3/0627 ,  A61B5/1459 ,  H01S3/025 ,  H01S3/091 ,  H01S3/102 ,  H01S3/1028 ,  H01S3/105 ,  H01S3/115 ,  H01S5/0607 ,  H01S5/0612 ,  H01S5/1042 ,  H01S5/3013

Abstract: A microlaser system includes an optical source, a microlaser, an actuator switch, and a photovoltaic power source. The microlaser, which includes a control element, is optically pumped by at least a portion of light emitted by the optical source. The actuator switch is configured to be activated by a triggering event. Furthermore, the photovoltaic power source is coupled in a series connection with the actuator switch and the control element, the series connection configured to connect the photovoltaic power source to the control element of the microlaser when the actuator switch is activated by the triggering event.