公开(公告)号：US20240167105A1

公开(公告)日：2024-05-23

申请号：US18419486

申请日：2024-01-22

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Mark D. GOLDBERG ,  Erika F. GARCIA ,  Xiomara L. MADERO ,  Thomas A. TOMBRELLO ,  Axel SCHERER

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20190127799A1

公开(公告)日：2019-05-02

申请号：US16102583

申请日：2018-08-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER ,  Mark D. GOLDBERG

IPC: C12Q1/6883 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US20150167092A1

公开(公告)日：2015-06-18

申请号：US14633094

申请日：2015-02-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER ,  Mark D. GOLDBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

Abstract translation: 描述了基于FRET的分析物检测和相关方法和系统，其中使用一对FRET标记的引物和/或寡核苷酸，其特异于靶向序列，其位于与FRET上呈现的FRET发色团的Förster距离的四倍 在一个或多个多核苷酸分析物中相对于另一个标记的引物和/或寡核苷酸; 特别地，一对FRET标记的引物和/或寡核苷酸与样品组合，并在测量来自至少一种FRET生色团的FRET信号之前进行一个或多个多核苷酸扩增反应。

公开(公告)号：US20140256114A1

公开(公告)日：2014-09-11

申请号：US14186839

申请日：2014-02-21

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Sameer WALAVALKAR ,  Mark D. GOLDBERG ,  Axel SCHERER

IPC: H01L21/762

CPC classification number: H01L21/76283 ,  H01L21/762

Abstract: Novel methods to fabricate biological sensors and electronics are disclosed. A silicon-on-insulator wafer can be employed by etching a pattern of holes in the silicon layer, then a pattern of cavities in the insulating layer, and then sealing the top of the cavities. Further, n or p doped regions and metallic regions can be defined in the processed wafer, thereby enabling integration of biological sensing and electronic capabilities in the same wafer.

Abstract translation: 公开了制造生物传感器和电子学的新方法。 可以通过蚀刻硅层中的孔的图案，然后在绝缘层中蚀刻空腔的图案，然后密封空腔的顶部来采用绝缘体上硅晶片。 此外，可以在经处理的晶片中限定n或p个掺杂区域和金属区域，从而使生物感测和电子能力能够集成在相同的晶片中。

公开(公告)号：US20140213471A1

公开(公告)日：2014-07-31

申请号：US14162725

申请日：2014-01-23

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Mark D. GOLDBERG ,  Erika F. GARCIA ,  Xiomara L. MADERO ,  Thomas A. TOMBRELLO ,  Axel SCHERER

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.

Abstract translation: 本公开提供了用于检测样品中多种分析物的方法，组合物和试剂盒。 在一些实例中，本公开提供了用于检测分析物，遗传变异，监测反应过程以及通过测量信号监测分析物 - 分析物相互作用的方法，组合物和试剂盒。 在一些示例中，可以使用信号的存在或信号的改变来构建可用于检测分析物的信号谱。