公开(公告)号：US20190127799A1

公开(公告)日：2019-05-02

申请号：US16102583

申请日：2018-08-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER ,  Mark D. GOLDBERG

IPC: C12Q1/6883 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US20150038379A1

公开(公告)日：2015-02-05

申请号：US14511778

申请日：2014-10-10

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Emil P. KARTALOV ,  Darryl SHIBATA ,  Clive TAYLOR ,  Lawrence A. WADE

IPC: C12M1/42 ,  C12M1/32 ,  C12M3/06 ,  C12M1/12

CPC classification number: C12M23/16 ,  C12M23/12 ,  C12M23/22 ,  C12M25/04 ,  C12M25/14 ,  C12M35/02 ,  C12M37/04 ,  C12M47/04 ,  C12N13/00 ,  G01N33/54366

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.

Abstract translation: 本文提供用于微生物细胞材料的微分离的装置和方法。 所描述的微分离装置可以包括保护感兴趣区域以防止DNA破坏照明的光掩模。 微型隔离装置还可以包括感光材料，该感光材料在照射之后界定存取孔，随后显影感光材料。 微隔离装置还可包括腔室微流体装置，其包括通道，其提供对感光材料中限定的孔的通路。 微型隔离装置可以包括腔室微流体装置，其无感光材料中限定的通路孔，阀门通过微流体装置的通道控制气体或液体的流动。 还包括使用本文所述装置选择性分离细胞材料的方法，以及使用本文所述装置的细胞材料的各个感兴趣区域的生物化学分析的方法。 还包括制备用于本文所述方法的掩蔽阵列的方法。

公开(公告)号：US20200216155A1

公开(公告)日：2020-07-09

申请号：US16719371

申请日：2019-12-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Axel SCHERER

IPC: B63C11/02 ,  G01N21/41 ,  G01N27/22

Abstract: Methods to build implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring physical changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The teachings of the disclosure help improve safety and efficiency of diving operations.

公开(公告)号：US20180171331A1

公开(公告)日：2018-06-21

申请号：US15885736

申请日：2018-01-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA ,  CURATE BIOSCIENCES LLC

Inventor： Emil P. KARTALOV ,  Cheng-Chung LEE ,  Paul PREDKI

IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/686 ,  B01L3/00

CPC classification number: C12N15/1093 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2300/0887 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2563/179

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the devices described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein. The micro-isolation devices can comprise a unique combination of barcodes in each microfluidics well, allowing two-dimensional mapping of genetic information.

公开(公告)号：US20130267005A1

公开(公告)日：2013-10-10

申请号：US13913321

申请日：2013-06-07

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA ,  CALIFORNIA INSTITUTE OF TECHNOLOGY ,  APIC CORPORATION

Inventor： Emil P. KARTALOV ,  Axel SCHERER ,  Koichi SAYANO

IPC: B01L3/00

CPC classification number: B01L3/502753 ,  A61M1/3472 ,  A61M1/3496 ,  A61M1/3633 ,  A61M2205/0244 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0424 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided.

Abstract translation: 描述了用于通过过滤分离流体的目标成分的微流体流体分离器。 提供了通过过滤分离流体的目标组分的方法和用微流体分离器大规模处理血液的方法。

公开(公告)号：US20240182954A1

公开(公告)日：2024-06-06

申请号：US18352080

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/64 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10

CPC classification number: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/6486 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20160208242A1

公开(公告)日：2016-07-21

申请号：US14996613

申请日：2016-01-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA ,  CURATE BIOSCIENCES LLC

Inventor： Emil P. KARTALOV ,  Cheng-Chung LEE ,  Paul PREDKI

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1093 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2300/0887 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2563/179

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the devices described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein. The micro-isolation devices can comprise a unique combination of barcodes in each microfluidics well, allowing two-dimensional mapping of genetic information.

Abstract translation: 本文提供用于微生物细胞材料的微分离的装置和方法。 所描述的微隔离装置可以包括保护感兴趣区域以防止DNA破坏照明的光掩模。 微型隔离装置还可以包括光敏材料，该感光材料在照明之后界定存取孔，随后显影感光材料。 微隔离装置还可以包括腔室微流体装置，其包括通道，其提供对感光材料中限定的孔的通路。 微隔离装置可以包括腔室微流体装置，其中光敏材料中没有限定孔，其中阀控制通过微流体装置的通道的气体或液体的流动。 还包括使用本文所述装置选择性分离细胞材料的方法，以及使用本文所述装置的细胞材料的各个感兴趣区域的生物化学分析的方法。 还包括制备用于本文所述方法的掩蔽阵列的方法。 微隔离装置可以包括每个微流体阱中的条形码的独特组合，允许遗传信息的二维映射。

公开(公告)号：US20140096831A1

公开(公告)日：2014-04-10

申请号：US13840440

申请日：2013-03-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  John LIU ,  Axel SCHERER ,  Clive TAYLOR

IPC: G05D7/06

CPC classification number: G05D7/0694 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0057 ,  F16K2099/008 ,  Y10T137/0318 ,  Y10T137/218 ,  Y10T137/2202 ,  Y10T137/2224 ,  Y10T137/7795 ,  Y10T137/7796

Abstract: Multi-valve autoregulatory microfluidic devices and methods are described. The described devices and methods offer improved performance and new means of tuning autoregulatory effects in microfluidic devices.

Abstract translation: 描述了多瓣膜自动调节微流体装置和方法。 所描述的装置和方法提供改进的性能和调整微流体装置中的自动调节效应的新手段。

公开(公告)号：US20250084463A1

公开(公告)日：2025-03-13

申请号：US18893086

申请日：2024-09-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/64 ,  G06F17/10 ,  G06F17/11 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/00 ,  G16B40/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20240093273A1

公开(公告)日：2024-03-21

申请号：US18516842

申请日：2023-11-21

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/64 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10

CPC classification number: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/6486 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.