公开(公告)号：US20120126143A1

公开(公告)日：2012-05-24

申请号：US13378752

申请日：2010-06-02

申请人： Michael Himmelhaus

发明人： Michael Himmelhaus

IPC分类号： G01N21/64

CPC分类号： G01N21/7746 ,  G01N21/648 ,  G01N2021/6417 ,  G01N2021/7786 ,  G01N2201/062

摘要： An optical cavity mode apparatus comprising at least one microresonator; a light emitting diode for supplying light irradiation to the microresonator to stimulate the excitation level of the microresonator; and an optical detector to obtain spectra of the microresonator stimulated by the light emitting diode.

摘要翻译： 一种光腔模式设备，包括至少一个微谐振器; 用于向微谐振器提供光照射以刺激微谐振器的激发电平的发光二极管; 以及光学检测器，以获得由发光二极管激发的微谐振器的光谱。

公开(公告)号：US20110306132A1

公开(公告)日：2011-12-15

申请号：US13202713

申请日：2010-02-18

申请人： Yoshiyuki Hotta

发明人： Yoshiyuki Hotta

IPC分类号： C12N5/077

CPC分类号： C12N5/0654 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/39

摘要： Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of bone cells under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into bone cells comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into bone cells, a ligand for endothelial cell differentiation gene (Edg) family receptors and selenium, which culture medium is serum-free or contains a low concentration of serum. The method for inducing differentiation from somatic stem cells into bone cells comprises culturing the somatic stem cells that can differentiate into bone cells in the above-described culture medium.

摘要翻译： 公开了一种培养基，添加剂和用于在无血清或低血清培养基条件下有效诱导哺乳动物体细胞干细胞分化为具有骨细胞特征的细胞的方法。 用于诱导哺乳动物体细胞干细胞分化成骨细胞的培养基包括用于培养哺乳动物细胞的基础培养基，用于诱导哺乳动物体细胞干细胞分化为骨细胞的试剂，内皮细胞分化基因（Edg）家族的配体 受体和硒，培养基是无血清或含有低浓度的血清。 用于诱导从体细胞干细胞分化成骨细胞的方法包括培养可以分化成上述培养基中的骨细胞的体细胞干细胞。

公开(公告)号：US20110019186A1

公开(公告)日：2011-01-27

申请号：US12811166

申请日：2008-12-25

申请人： Michael Himmelhaus ,  Alexandre Francois

发明人： Michael Himmelhaus ,  Alexandre Francois

IPC分类号： G01J3/30

CPC分类号： G01N21/7703 ,  G01N21/05 ,  G01N21/274 ,  G01N21/648 ,  G01N2021/0346 ,  G01N2021/7789 ,  G01N2201/129

摘要： A method for sensing a target object using optical mode excitations in microresonators, comprises: preparing at least one cluster including at least two microresonators; obtaining some first spectra of the cluster; adsorbing the target object on a surface of the cluster; obtaining some second spectra of the cluster; and sensing the target object by comparing a lineshape of the first spectra with a lineshape of the second spectra.

摘要翻译： 一种用于在微谐振器中使用光学模式激励来感测目标物体的方法，包括：制备至少一个包括至少两个微谐振器的簇; 获得集群的一些第一个光谱; 将所述目标物体吸附在所述簇的表面上; 获得集群的一些第二个光谱; 并且通过将第一光谱的线形与第二光谱的线形比较来感测目标对象。

公开(公告)号：US20100086992A1

公开(公告)日：2010-04-08

申请号：US12518156

申请日：2007-12-21

申请人： Michael Himmelhaus ,  Spencer Spratt

发明人： Michael Himmelhaus ,  Spencer Spratt

IPC分类号： C12M1/34 ,  B05D3/00

CPC分类号： G01N33/54366 ,  G01N33/54353 ,  G01N33/554

摘要： A biosensor chip for sensing a target molecule, includes: a substrate having a surface with a sensing area; and adhesive material for immobilizing a mollicute having a cell membrane on the sensing area. The chip may includes cell-resistant material for preventing the mollicute from being immobilized on those parts of the surface of the substrate that do not belong to the sensing area. Further, the adhesive material may comprise a first adhesive material for immobilizing a body of the mollicute on the sensing area and a second adhesive material for immobilizing a tip of the mollicute on the surface of the substrate.

摘要翻译： 用于感测目标分子的生物传感器芯片包括：具有感测区域的表面的基板; 以及用于将具有细胞膜的摩擦固定在感测区域上的粘合材料。 芯片可以包括用于防止摩擦固定在不属于感测区域的基底表面的那些部分上的耐细胞材料。 此外，粘合剂材料可以包括用于将摩擦体的主体固定在感测区域上的第一粘合材料和用于将摩擦的尖端固定在基底表面上的第二粘合材料。

公开(公告)号：US07488453B2

公开(公告)日：2009-02-10

申请号：US10722126

申请日：2003-11-26

申请人： Hiroyuki Takahashi ,  Eiichiro Tanaka ,  Hiroyuki Nakamura

发明人： Hiroyuki Takahashi ,  Eiichiro Tanaka ,  Hiroyuki Nakamura

IPC分类号： B01L3/00

CPC分类号： B65D47/0866 ,  B01L3/50825 ,  B65D2251/1075 ,  G01N35/1002 ,  Y10T436/2575

摘要： A reagent vessel cap includes a sealing member for sealing the opening of a vessel body; an pressurizing member linked to a retaining part for sealing the sealing member, for bringing the sealing member in close contact with the opening all the time, wherein when pressure is applied, the pressurizing member lifts the sealing member against the biasing force of itself to open the vessel and, when the pressure is eliminated, the pressurizing member returns to position by the biasing force to close the vessel by the sealing member; and a cap body capable of mounting the sealing member and the pressurizing member to the opening of the vessel body containing a reagent. The reagent is collected using the cap structure.

摘要翻译： 试剂容器盖包括用于密封容器主体的开口的密封构件; 连接到用于密封密封构件的保持部分的加压构件，用于使密封构件始终与开口紧密接触，其中当施加压力时，加压构件抵抗其自身的偏压力提升密封构件以打开 并且当压力消除时，加压构件通过偏置力返回到位置，以通过密封构件关闭容器; 以及能够将密封构件和加压构件安装到容纳有试剂的容器主体的开口的盖体。 使用盖结构收集试剂。

公开(公告)号：US20070059786A1

公开(公告)日：2007-03-15

申请号：US11447839

申请日：2006-06-07

申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

IPC分类号： G01N33/551 ,  C07D305/02

CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

公开(公告)号：US20050239149A1

公开(公告)日：2005-10-27

申请号：US10518586

申请日：2003-06-24

申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

IPC分类号： G01N21/76 ,  G01N33/532 ,  G01N33/533 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  C07D35/02

CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理和化学发光 方法和使用化学发光增强剂的试剂盒。

公开(公告)号：US06919435B1

公开(公告)日：2005-07-19

申请号：US09350899

申请日：1999-07-12

申请人： Kouichi Tsuji ,  Yoshihiro Yoshida ,  Takashi Uwano

发明人： Kouichi Tsuji ,  Yoshihiro Yoshida ,  Takashi Uwano

IPC分类号： A61K39/00 ,  A61K39/395 ,  A61P35/00 ,  C07K14/435 ,  C07K14/705 ,  C07K16/00 ,  C07K16/30 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  G01N33/574 ,  G01N33/577

CPC分类号： C07K14/435 ,  C07K16/3023 ,  G01N33/57423 ,  Y10S530/828

摘要： The monoclonal antibody produced by a hybridoma cell strain TRD-L1, -L2 or -L3 which is obtained by the fusion between mouse myeloma cells and spleen cells of a mouse immunized with a human lung adenocarcinoma cell secretion component reacts in a specific fashion with a glycoprotein antigen that has a moecular weight of 200 kD or more (SDS-PAGE) and is present in human lung adenocarcinoma cells. It can be used efficiently in cancer diagnosis.

摘要翻译： 通过小鼠骨髓瘤细胞与用人肺腺癌细胞分泌成分免疫的小鼠的脾细胞融合获得的杂交瘤细胞株TRD-L1，-L2或-L3产生的单克隆抗体以特定方式与 糖蛋白抗原具有200kD以上的摩尔体重（SDS-PAGE），并存在于人肺腺癌细胞中。 它可以有效地用于癌症诊断。

公开(公告)号：US5859244A

公开(公告)日：1999-01-12

申请号：US855500

申请日：1997-05-13

申请人： Hiroshi Ikawa ,  Akiyoshi Kadoiri ,  Yasuko Konagai ,  Tetsuaki Yamaura ,  Noriko Kase

发明人： Hiroshi Ikawa ,  Akiyoshi Kadoiri ,  Yasuko Konagai ,  Tetsuaki Yamaura ,  Noriko Kase

IPC分类号： A61K31/435 ,  A61K31/4422 ,  A61K31/4427 ,  A61K31/495 ,  A61P7/02 ,  A61P9/08 ,  A61P9/12 ,  A61P11/08 ,  A61P25/28 ,  A61P29/00 ,  A61P37/08 ,  C07D211/90 ,  C07D213/82 ,  C07D401/06 ,  C07D401/12 ,  C07D401/14 ,  C07D405/14 ,  C07D409/14 ,  C07D413/12 ,  C07D417/12 ,  C07D473/08 ,  C07F7/10 ,  C07D213/12 ,  C07D401/04 ,  C07D403/04

CPC分类号： C07D401/06 ,  C07D211/90 ,  C07D213/82 ,  C07D401/12

摘要： 1,4-dihydropyridine derivatives and optically active 1,4-dihydropyridine derivatives with the following formula, having vasodilating activity based on calcium antagonism, and PAF antaognism, and methods of producing the same are disclosed: ##STR1## wherein (*) indicates a chiral center in the case of the optically active 1,4-dihydropyridine derivatives.

摘要翻译： 公开了具有下式的1,4-二氢吡啶衍生物和光学活性的1,4-二氢吡啶衍生物，其具有基于钙拮抗作用的血管舒张活性和PAF抗体，其制备方法：其中（*）表示 在光学活性的1,4-二氢吡啶衍生物的情况下手性中心。

公开(公告)号：US5470759A

公开(公告)日：1995-11-28

申请号：US73340

申请日：1993-06-07

申请人： Masami Sugiyama ,  Yoshiaki Uchida ,  Yoshihiro Kurano ,  Aiko Tanaka ,  Tetsuji Tanimoto

发明人： Masami Sugiyama ,  Yoshiaki Uchida ,  Yoshihiro Kurano ,  Aiko Tanaka ,  Tetsuji Tanimoto

IPC分类号： G01N33/50 ,  C07K16/18 ,  C12P21/08 ,  G01N33/72 ,  G01N33/538

CPC分类号： C07K16/18 ,  G01N33/723

摘要： A method for measuring glycated hemoglobin is disclosed, which comprises contacting a sample with an anti-hemoglobin antibody being bound to a solid phase, adding thereto an anti-glycated hemoglobin antibody, and then measuring the anti-glycated hemoglobin antibody bound to the solid phase via glycated hemoglobin and the anti-hemoglobin antibody. A method for measuring glycated hemoglobin is further disclosed, which comprises mixing a sample with particles on which an anti-glycated hemoglobin antibody is bound to cause immunoagglutination reaction. A method for measuring glycated hemoglobin is furthermore disclosed, which comprises mixing a sample with a solution containing a monoclonal antibody specific for glycated hemoglobin, and then measuring the change in the turbidity of the resulting mixture. A method is disclosed for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and an anti-glycated hemoglobin antibody, to cause immunoagglutination reaction. There is disclosed a method for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and particles on which an anti-glycated hemoglobin antibody is bound, to cause immunoagglutination reaction. Still furthermore, an anti-glycated hemoglobin monoclonal antibody having a high specificity for human glycated hemoglobin is disclosed.

摘要翻译： 公开了一种测定糖化血红蛋白的方法，其包括将样品与结合于固相的抗血红蛋白抗体接触，向其中加入抗糖化血红蛋白抗体，然后测定与固相结合的抗糖化血红蛋白抗体 通过糖化血红蛋白和抗血红蛋白抗体。 进一步公开了测定糖化血红蛋白的方法，其包括将样品与其上结合有抗糖基化血红蛋白抗体的颗粒混合以引起免疫凝集反应。 还公开了一种测定糖化血红蛋白的方法，其包括将样品与含有糖化血红蛋白特异性的单克隆抗体的溶液混合，然后测量所得混合物的浊度变化。 公开了一种用于测量糖化血红蛋白的方法，其包括将样品与未敏化的胶乳颗粒和抗糖化血红蛋白抗体混合以引起免疫凝集反应。 公开了一种测定糖化血红蛋白的方法，其包括将样品与未敏化的胶乳颗粒和抗糖基化血红蛋白抗体结合的颗粒混合，引起免疫凝集反应。 此外，公开了对人糖化血红蛋白具有高特异性的抗糖化血红蛋白单克隆抗体。