公开(公告)号：US20100032582A1

公开(公告)日：2010-02-11

申请号：US12187815

申请日：2008-08-07

Applicant: Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

Inventor： Hua Xia ,  Li Zhu ,  Eugene Barash ,  Erin Jean Finehout ,  Matthew Damian Pietrzykowski

IPC: G01N21/64

CPC classification number: G01N21/645 ,  B01L3/5027 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/08 ,  G01J3/4406 ,  G01N21/6408 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A fluorescence detection system comprises a light source configured to produce an excitation light, an optical lens and a fiber bundle. The optical lens is configured to focus the excitation light to a sample to emit fluorescence and to collect the fluorescence. The fiber bundle probe comprises a transmitting fiber configured to transmit the excitation light to the optical lens, and a first receiving fiber configured to deliver the collected fluorescence. The fluorescence detection system further comprises a first detector configured to detect the fluorescence delivered by the receiving fiber to generate a response signal, and a processing unit configured to determine information about the samples by analyzing the response signal. Additionally, a fluorescence detection method is also presented.

Abstract translation: 荧光检测系统包括被配置为产生激发光，光学透镜和光纤束的光源。 光学透镜被配置为将激发光聚焦到样品以发射荧光并收集荧光。 纤维束探针包括被配置为将激发光透射到光学透镜的发射光纤和被配置为传送所收集的荧光的第一接收光纤。 荧光检测系统还包括第一检测器，其被配置为检测由接收光纤传送的荧光以产生响应信号;以及处理单元，被配置为通过分析响应信号来确定关于样本的信息。 另外，还提出了荧光检测方法。

公开(公告)号：US20090317856A1

公开(公告)日：2009-12-24

申请号：US12479600

申请日：2009-06-05

Applicant: Mary-Ann Mycek ,  Malavika Chandra ,  James Scheiman ,  Robert H. Wilson ,  Diane Simeone ,  Barbara McKenna ,  Julianne Purdy

Inventor： Mary-Ann Mycek ,  Malavika Chandra ,  James Scheiman ,  Robert H. Wilson ,  Diane Simeone ,  Barbara McKenna ,  Julianne Purdy

IPC: C12Q1/02 ,  C12M1/34

CPC classification number: G01N33/57438 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/415 ,  A61B5/418 ,  A61B5/7264 ,  A61B5/7267 ,  G01J3/10 ,  G01J3/4406 ,  G01N21/474 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6486 ,  G01N2021/4742 ,  G01N2021/6484 ,  G01N2201/1293

Abstract: Multimodal optical spectroscopy systems and methods produce a spectroscopic event to obtain spectroscopic response data from biological tissue and compare the response data with preset criteria configured to correlate the measured response data and the most probable attributes of the tissue, thus facilitating classification of the tissue based on those attributes for subsequent biopsy or remedial measures as necessary.

Abstract translation: 多光谱光谱系统和方法产生光谱事件以从生物组织获得光谱响应数据，并将响应数据与配置为将测量的响应数据和组织的最可能属性相关联的预设标准进行比较，从而便于基于 这些属性用于随后的活检或必要的补救措施。

公开(公告)号：US07608841B2

公开(公告)日：2009-10-27

申请号：US11451903

申请日：2006-06-12

Applicant: Taylor A. Reid ,  Roger H. Taylor ,  Kenneth J. Zajac

Inventor： Taylor A. Reid ,  Roger H. Taylor ,  Kenneth J. Zajac

IPC: G01N21/64

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光发射到照明管中的光源; 多个收集光学器件，位于照明管中用于收集荧光的孔周围; 以及用于确定荧光量的检测器。 检测荧光的方法包括将来自光源的激发光发射到照明管中; 将激发光引导到激发滤光器; 用激发光照射样品以产生发射光; 以及使用位于照明管周围的多个收集光学器件来检测发射光的光学特性。

公开(公告)号：US07605976B1

公开(公告)日：2009-10-20

申请号：US11416455

申请日：2006-05-03

Applicant: Ralf Wolleschensky ,  Michael Kempe ,  Peter Klopfleisch

Inventor： Ralf Wolleschensky ,  Michael Kempe ,  Peter Klopfleisch

IPC: G02B21/06

CPC classification number: G01J3/4406 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0224 ,  G01J3/0229 ,  G01J3/14 ,  G01N21/21 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2021/6417 ,  G02B21/0068 ,  G02B21/0076 ,  G02B26/0816 ,  G02B27/143 ,  G02B27/283

Abstract: Method and device for changing the illumination light and/or specimen light with respect to its spectral composition and/or intensity in an adjustable manner, wherein a spatial separation into the radiation components of different polarization is carried out with the first polarization means (Pol 1), a spectral spatial splitting of at least one radiation component is carried out with the first dispersion means (Disp1), and the polarization state of at least one part of the spectrally spatially split radiation component is changed, wherein a reflection of the illumination light and/or the detection light is carried out.

Abstract translation: 用于以可调节的方式改变照明光和/或样本光的方法和装置，其中利用第一偏振装置（Pol 1）执行不同偏振的辐射分量的空间分离 ），利用第一色散装置（Disp1）执行至少一个辐射分量的光谱空间分裂，并且改变光谱空间分裂的辐射分量的至少一部分的偏振状态，其中照明光的反射 和/或检测光。

公开(公告)号：US20090257107A1

公开(公告)日：2009-10-15

申请号：US12318287

申请日：2008-12-23

Applicant: Ralf Wolleschensky

Inventor： Ralf Wolleschensky

IPC: G02F1/11 ,  G02B21/06

CPC classification number: G02B27/283 ,  G01J3/0224 ,  G01J3/1256 ,  G01J3/4406 ,  G01J3/447 ,  G01N21/6445 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/0068

Abstract: Device for controlling light radiation, which is excited in a specimen and/or which is backscattered and/or reflected and which contains one or more wavelengths, at a plurality of light outlets, wherein a separation of the light radiation into differently polarized components is carried out; and the components of the excitation radiation and/or detection radiation are affected in their polarization by means of a preferably birefringent, preferably acousto-optic or electro-optic medium, which changes the ordinary and extraordinary refractive index.

Abstract translation: 用于控制在多个光出口处在样本中激发并且/或背散射和/或反射并且包含一个或多个波长的光辐射的装置，其中将光辐射分离成不同偏振的分量 出来 并且激发辐射和/或检测辐射的分量通过改变普通和非凡折射率的优选双折射，优选声光或电光介质在它们的极化中受到影响。

公开(公告)号：US20090190822A1

公开(公告)日：2009-07-30

申请号：US12362170

申请日：2009-01-29

Applicant: William E. Ortyn ,  David A. Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

Inventor： William E. Ortyn ,  David A. Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

IPC: G06K9/00

CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

公开(公告)号：US20090156429A1

公开(公告)日：2009-06-18

申请号：US12388358

申请日：2009-02-18

Applicant: Graham B.I. Scott ,  Carter Kittrell ,  Robert F. Curl ,  Michael Metzker

Inventor： Graham B.I. Scott ,  Carter Kittrell ,  Robert F. Curl ,  Michael Metzker

IPC: C40B30/10 ,  C12Q3/00

CPC classification number: G01J3/4406 ,  G01J3/021 ,  G01J3/10 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6441

Abstract: The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

Abstract translation: 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。

公开(公告)号：US07541176B2

公开(公告)日：2009-06-02

申请号：US11677765

申请日：2007-02-22

Applicant: Jeffrey Raynor

Inventor： Jeffrey Raynor

IPC: C12M1/34

CPC classification number: G01J3/4406 ,  G01J1/46 ,  G01J3/02 ,  G01J3/027 ,  G01J3/2803 ,  G01N21/6428 ,  G01N21/645

Abstract: A fluorescence detector includes a light source being positioned so that in use, radiation emitted therefrom falls on one or more fluorescing species, and a photodetector being switchably connectable between a charge integrator device and a charge disposal device. The photodetector is positionable to detect fluorescent radiation from the fluorescing species. A controller communicates with the light source and the photodetector, and is operable in a cyclic manner to activate the light source to emit radiation and connect the photodetector to the charge disposal device for a first period of time, and connect the photodetector to the charge integrator device for a second period of time after the first period. The charge integrator device is not reset from one cycle to the next.

Abstract translation: 荧光检测器包括光源，其定位使得在使用中，从其发射的辐射落在一个或多个荧光物质上，并且光电检测器可在电荷积分器装置和电荷处理装置之间可切换地连接。 光电检测器可定位以检测荧光物种的荧光辐射。 控制器与光源和光电检测器通信，并且以循环的方式操作以激活光源以发射辐射并且将光电检测器连接到电荷处理装置一段时间，并将光电检测器连接到电荷积分器 设备在第一时段之后的第二时段。 电荷积分器器件不会从一个周期复位到下一个周期。

公开(公告)号：US20090006004A1

公开(公告)日：2009-01-01

申请号：US12159005

申请日：2006-12-27

Applicant: Rudiger Sens ,  Christos Vamvakaris ,  Wolfgang Ahlers ,  Erwin Thiel

Inventor： Rudiger Sens ,  Christos Vamvakaris ,  Wolfgang Ahlers ,  Erwin Thiel

IPC: G01N21/00

CPC classification number: G01J3/4406 ,  G01J3/02 ,  G01J3/0232 ,  G01J3/457 ,  G01J2001/4242 ,  G01N21/31 ,  G01N21/643 ,  G01N21/645 ,  G01N2021/6491 ,  G01N2201/129

Abstract: A method is proposed for detecting at least one chemical compound V contained in a medium (312). The method comprises a verification step (420) which is used to determine whether V is contained in the medium (312). The method furthermore comprises an analysis step (424) in which a concentration c of the at least one chemical compound V is determined.The verification step comprises the following substeps: (a1) the medium (312) is exposed to a first analysis radiation (316) of a variable wavelength λ, the wavelength λ assuming at least two different values; (a2) at least one spectral response function A(λ) is generated with the aid of the radiation (324) absorbed and/or emitted and/or reflected and/or scattered by the medium (312) in response to the first analysis radiation (316); (a3) at least one spectral correlation function K(δλ) is formed by spectral comparison of the at least one spectral response function A(λ) with at least one pattern function R(λ+δλ), the at least one pattern function R(λ) representing a spectral measurement function of a medium (312) containing the chemical compound V and δλ being a coordinate shift; (a4) the at least one spectral correlation function K(δλ) is examined in a pattern recognition step (418), and a conclusion is made as to whether the at least one chemical compound V is contained in the medium (312); The analysis step (424) comprises the following substeps: (b1) the medium (312) is exposed to at least one second analysis radiation (318) having at least one excitation wavelength λEX; (b2) at least one spectral analysis function B(λEX,λRES) is generated with the aid of the radiation (326) of the response wavelength λRES absorbed and/or emitted and/or reflected and/or scattered by the medium (312) in response to the second analysis radiation (318) of the wavelength λEX and the concentration c is deduced therefrom.

公开(公告)号：US07463357B2

公开(公告)日：2008-12-09

申请号：US11290100

申请日：2005-11-29

Applicant: John C. Kralik ,  Tariq N. Faiz

Inventor： John C. Kralik ,  Tariq N. Faiz

IPC: G01J3/30

CPC classification number: G01J3/30 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/06 ,  G01J3/4406 ,  G01N21/6445 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/1241

Abstract: A system for detecting a predetermined wavelength of light emitted from an area on the surface of a chemical array is provided. Aspects of the system include: a beam splitter for splitting emitted light into two or more light beams and a detector for detecting photons in each of the two produced light beams. Methods of detecting light using the subject system, programming for performing the subject methods and an array reader containing the subject system are also provided.

Abstract translation: 提供一种用于检测从化学阵列表面上的区域发射的预定波长的光的系统。 该系统的方面包括：用于将发射的光分成两个或更多个光束的分束器和用于检测两个产生的光束中的每一个中的光子的检测器。 还提供了使用主题系统检测光的方法，用于执行主题方法的编程以及包含该主题系统的阵列阅读器。