公开(公告)号：US11967496B2

公开(公告)日：2024-04-23

申请号：US17580917

申请日：2022-01-21

Applicant: Fluidigm Canada Inc. ,  University of Ottawa

Inventor： Paul Corkum ,  Alexander V. Loboda

IPC: H01J49/04 ,  G01N33/68 ,  G02B21/33 ,  H01J37/20 ,  H01J37/28 ,  H01J49/00 ,  H01J49/02 ,  H01J49/06 ,  H01J49/10 ,  H01J49/14 ,  H01J49/16 ,  H01J49/40

CPC classification number: H01J49/0463 ,  G01N33/6848 ,  G01N33/6851 ,  G02B21/33 ,  H01J37/20 ,  H01J37/28 ,  H01J49/0004 ,  H01J49/0031 ,  H01J49/025 ,  H01J49/0418 ,  H01J49/067 ,  H01J49/105 ,  H01J49/142 ,  H01J49/161 ,  H01J49/164 ,  H01J49/40

Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC™) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.

公开(公告)号：US20230154739A1

公开(公告)日：2023-05-18

申请号：US17916646

申请日：2021-04-01

Applicant: ISTITUTO NAZIONALE DI FISICA NUCLEARE

Inventor： Giovanni CARUGNO

IPC: H01J49/16 ,  H01J49/40

CPC classification number: H01J49/161 ,  H01J49/40

Abstract: The embodiments relate to a method and an apparatus for the molecular atomic analysis of a fluid in the gaseous state, in particular the method includes introducing a fluid in the gaseous state into a collection chamber having a predetermined internal volume V and generating a laser beam through a laser device. The method may also include focusing the beam onto the fluid sited in the collection chamber, in order to create an electric field in at least a portion V′ of the internal volume V, so as to excite the electrons residing on the atoms and molecules present in said fluid in the gaseous state, causing an atomic/molecular alteration of the fluid itself in said portion V′. The method provides detecting the elements emitted after focusing the beam on the fluid, through detection devices and analyzing the elements detected by the detection devices using a processing unit.

公开(公告)号：US20180067136A1

公开(公告)日：2018-03-08

申请号：US15717246

申请日：2017-09-27

Applicant: Atomic Oncology Pty Ltd

Inventor： George L. Gabor Miklos

IPC: G01N33/84 ,  G01N33/574

CPC classification number: G01N33/84 ,  G01N21/3103 ,  G01N21/718 ,  G01N22/00 ,  G01N23/085 ,  G01N23/223 ,  G01N33/574 ,  G01N2223/402 ,  G01N2223/6126 ,  G01N2800/52 ,  H01J49/161

Abstract: The present invention relates to the generation of an Atomic Therapeutic Indicator (ATI) for a test sample by the quantification of manganese; in voxels of a 3D region of the sample, wherein the 3D region is topographically defined by co-ordinates X′xY′xZ. The ATI is used to assess the radio-responsiveness i.e. sensitivity or resistance to radiation treatment, of a cancer i.e. a tumour/neoplasm. In a preferred embodiment, the present invention relates to a method of generating the ATI, assessing the radio-responsiveness of a tumour/neoplasm based on the ATI and, based on the assessment, either treating or not treating the tumour with radiation.The present invention also relates to a method of determining if a cancer is likely to reoccur post radiation treatment comprising quantifying the level of manganese in voxels of a 3D region of a test sample from the cancer and determining the frequency of high metallomic regions (HMRs) in the cancer, wherein a high frequency of HMRs is indicative that the cancer is likely to reoccur and a low frequency of HMRs is indicative that the cancer is unlikely to reoccur; and associated methods of treatment.The invention further relates to a method of determining the radio-responsiveness of a melanoma, the method comprising determining the level of melanin in a test sample from the melanoma, wherein the lower the level of melanin the more sensitive the melanoma is to radiation and the higher the level of melanin the more resistant the melanoma is to radiation; and associated methods of treatment.

公开(公告)号：US20170309463A1

公开(公告)日：2017-10-26

申请号：US15135679

申请日：2016-04-22

Applicant: Morpho Detection, LLC

Inventor： Andrey N. Vilkov

IPC: H01J49/16 ,  G01N27/62 ,  H01J49/04 ,  H01J49/26

CPC classification number: H01J49/161 ,  G01N27/622 ,  G01N27/628 ,  H01J49/0409 ,  H01J49/107 ,  H01J49/162 ,  H01J49/26

Abstract: A dual source ionizer is provided. The dual source ionizer includes a first photoionization source configured to emit low flux ultraviolet (UV) light to generate primarily NO3− ions, and a second photoionization source configured to emit high flux UV light to generate primarily ions other than NO3− ions.

公开(公告)号：US09759685B2

公开(公告)日：2017-09-12

申请号：US14800410

申请日：2015-07-15

Applicant: Brechtel Manufacturing, Inc.

Inventor： Fredrick J Brechtel ,  Xerxes Lopez-Yglesias

IPC: H01J49/26 ,  G01N30/32 ,  G01N27/66 ,  G01R29/24 ,  H01J27/02 ,  G01N27/62 ,  H01J49/14 ,  H01J49/16

CPC classification number: G01N27/66 ,  G01N27/624 ,  G01R29/24 ,  H01J27/02 ,  H01J49/145 ,  H01J49/161

Abstract: A system and method comprising an ion production chamber having a ultra-violet light source disposed towards said chamber, a harvest gas disposed to flow through the chamber from an inlet to an outlet, and a jet, said jet operable to introduce a sample into the harvest gas flow. In some embodiments the system includes using helium as the harvest gas. Certain embodiments include introducing a sample perpendicular to the harvest gas flow and using multiple sample introduction jets to increase mixing efficiency. The charge sample may be coupled to a MEMS-based electrometer.

公开(公告)号：US09211542B2

公开(公告)日：2015-12-15

申请号：US13699446

申请日：2011-05-20

Applicant: Pawel Urban ,  Renato Zenobi ,  Konstantins Jefimovs ,  Andrea Amantonico ,  Stephan Fagerer ,  Nils Goedecke

Inventor： Pawel Urban ,  Renato Zenobi ,  Konstantins Jefimovs ,  Andrea Amantonico ,  Stephan Fagerer ,  Nils Goedecke

IPC: H01J49/00 ,  B01L9/00 ,  H01J49/04 ,  H01J49/16 ,  B05D3/00 ,  H01J49/26

CPC classification number: B01L9/00 ,  B05D3/007 ,  H01J49/0418 ,  H01J49/161 ,  H01J49/26

Abstract: A sample support plate (100) for a variety of possible applications, including MALDI mass spectrometry, is disclosed. A plurality of spatially separated sample recipient sites (101) are arranged on the surface of a substrate. The recipient sites are mutually separated by areas having a different wettability than the recipient sites. They are arranged in a plurality of rows consisting of a plurality of recipient sites whose centers are regularly spaced along a first direction with a predetermined periodicity (D1), the rows being regularly spaced along a second direction perpendicular to the first direction with a predetermined centerline distance (D2). Each recipient site has a maximum lateral dimension that is preferably smaller than the diameter of a beam spot (104) of a desorption laser beam (103). In order to enable unsupervised splitting of bulk liquid samples into droplets at the sample recipient sites, the periodicity along the first direction and the centerline distance along the second direction are chosen such that each recipient sites has a next neighbor at a distance that is less than or equal to three times the minimum lateral dimension of each recipient site. In preferred embodiments, the sample recipient sites are arranged in a checkerboard-type pattern or in rows that are inclined relative to the edges of the sample support plate.

Abstract translation: 公开了用于各种可能应用的样品支撑板（100），包括MALDI质谱法。 多个空间分离的样品接收部位（101）被布置在基板的表面上。 受体部位由具有与受体部位不同的润湿性的区域相互分开。 它们被布置成多个行，其由多个接收位点组成，其中心沿着第一方向以规定的周期（D1）规则地间隔开，行沿着与第一方向垂直的第二方向规则地间隔开预定的中心线 距离（D2）。 每个受体部位具有优选小于解吸激光束（103）的束斑（104）的直径的最大横向尺寸。 为了使样本接收点处的大量液体样品无监督分裂成液滴，选择沿着第一方向的周期性和沿第二方向的中心线距离，使得每个接收点位置具有距离小于 或等于每个接收者位置的最小横向尺寸的三倍。 在优选的实施方案中，样品受体部位以相对于样品支持板的边缘倾斜的棋盘型图案或行排列。

公开(公告)号：US09171708B1

公开(公告)日：2015-10-27

申请号：US14606495

申请日：2015-01-27

Applicant: Science and Engineering Services, LLC

Inventor： Vladimir M. Doroshenko ,  Victor V. Laiko ,  Eugene Moskovets

IPC: H01J49/16 ,  H01J49/26 ,  H01J49/04

CPC classification number: H01J49/161 ,  H01J49/0004 ,  H01J49/0404 ,  H01J49/107 ,  H01J49/164 ,  H01J49/24

Abstract: An interchangeable ion source for a spectrometer. The ion source includes an interface which mounts the ion source relative to a gas inlet of the spectrometer, a sample holder, a laser which produces a laser beam capable of ionizing the sample at ambient pressure, and an optical system. The ion source includes an equipment chassis which supports the interface, the sample holder, the laser and the optical system as a rigid unit such that the interface, the sample holder, the laser and the optical system remain in alignment upon attachment and detachment of the ion source from the spectrometer and an enclosure which embraces an atmosphere around components of the ion source. In addition, the ion source includes a circulator which circulates at least part of the atmosphere within the enclosure.

Abstract translation: 用于光谱仪的可互换的离子源。 离子源包括相对于光谱仪的气体入口安装离子源的接口，样品保持器，产生能够在环境压力下电离样品的激光束的激光器和光学系统。 离子源包括支撑界面的设备机架，样品架，激光器和光学系统作为刚性单元，使得接口，样品架，激光器和光学系统在安装和拆卸时保持对准 来自光谱仪的离子源和围绕离子源组件的气氛的外壳。 此外，离子源包括使外壳内的至少一部分大气循环的循环器。

公开(公告)号：US20150287581A1

公开(公告)日：2015-10-08

申请号：US14438246

申请日：2012-10-25

Applicant: MICROMASS UK LIMITED

Inventor： Stevan Bajic ,  David Douce ,  Gordon Jones

IPC: H01J49/04 ,  H01J49/06 ,  H01J49/00 ,  H01J49/16

CPC classification number: H01J49/045 ,  H01J49/0031 ,  H01J49/0445 ,  H01J49/0454 ,  H01J49/061 ,  H01J49/16 ,  H01J49/161

Abstract: An ion source is disclosed comprising a nebuliser and a target. The nebuliser is arranged and adapted to emit, in use, a stream of analyte droplets which are caused to impact upon the target and to ionise analyte to form a plurality of analyte ions. The target is vibrated by a piezo-electric vibration device to reduce the size of resultant secondary droplets.

Abstract translation: 公开了一种包括雾化器和靶的离子源。 雾化器布置并适于在使用中发射被分析物液滴流，其被引起撞击目标物体并使分析物离子化以形成多个分析物离子。 目标通过压电振动装置振动以减小所得到的二次液滴的尺寸。

公开(公告)号：US08981289B2

公开(公告)日：2015-03-17

申请号：US14125436

申请日：2011-12-16

Applicant: Seung Yong Kim ,  Mo Yang ,  Hyun Sik Kim

Inventor： Seung Yong Kim ,  Mo Yang ,  Hyun Sik Kim

IPC: H01J49/10 ,  H01J49/06 ,  H01J49/26 ,  H01J49/16 ,  H01J49/00 ,  H01J49/08 ,  H01J49/42

CPC classification number: H01J49/161 ,  H01J49/0022 ,  H01J49/08 ,  H01J49/424 ,  Y10T29/49826

Abstract: The present invention relates to an ultraviolet diode and an atomic mass analysis ionization source collecting device using an MCP. In the manufacturing of a portable atomic mass analyzer, an object of the present invention is to use an MCP electron multiplier plate, whereby ultraviolet photons emitted from an ultraviolet diode are irradiated on a front surface plate of the MCP electron multiplier plate to induce primary electrons, an amplified electron beam is collected from the electrons, and an electron beam is generated at a low temperature and low power and having a discharge time that is accurately controlled. The atomic mass analysis ionization source collecting device using an ultraviolet diode and an MCP according to the present invention comprises: an ultraviolet diode emitting ultraviolet rays by means of supplied power; an MCP electron multiplier plate inducing and amplifying primary electron discharge from ultraviolet photons from the ultraviolet diode, and collecting a large amount of electron beams from an MCP reverse surface plate; an electron condenser lens condensing the electron beam amplified through the MCP electron multiplier plate; an ion trap atomic mass separator ionizing gas sample molecules by means of an electron beam injected through the electron condenser lens; and an ion detector performing detection of ions separated from the ion trap atomic mass separator, by means of an atomic mass spectrum.

Abstract translation: 本发明涉及使用MCP的紫外二极管和原子质量分析电离源收集装置。 在便携式原子质量分析仪的制造中，本发明的目的是使用MCP电子倍增器板，其中从紫外二极管发射的紫外光子照射在MCP电子倍增器板的前表面板上以诱导一次电子 从电子收集放大的电子束，并且在低温和低功率下产生电子束，并且具有被精确控制的放电时间。 根据本发明的使用紫外二极管和MCP的原子质量分析电离源收集装置包括：通过供电的电源发射紫外线的紫外二极管; MCP电子倍增器板从紫外二极管的紫外光子中诱发和放大一次电子放电，并从MCP反面板收集大量的电子束; 电子聚光透镜，聚合通过MCP电子倍增器板放大的电子束; 离子阱原子质量分离器通过通过电子聚光透镜注入的电子束离子化气体样品分子; 以及离子检测器，其通过原子质谱进行离子阱原子质量分离器分离的离子的检测。

公开(公告)号：US20130221234A1

公开(公告)日：2013-08-29

申请号：US13777237

申请日：2013-02-26

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Akiko KAKUTANI ,  Kazuo HAYASHI ,  Akihiro OSANAI ,  Kiyokazu SATO ,  Takeshi YOSHIYUKI ,  Tsutomu KURUSU

IPC: H01J27/24

CPC classification number: H01J27/24 ,  H01J49/161 ,  H01J49/162 ,  H01J49/164

Abstract: According to one embodiment, there is provided a laser ion source. The laser ion source includes a vacuum chamber which is vacuum-exhausted and in which a target is transported and set, a valve which is opened when the target is transported into the vacuum chamber and is closed except for the transportation, a target supply chamber which holds the target to be movable, and a transportation unit which transports to the vacuum chamber the target held on the target supply chamber while opening the valve after the target supply chamber is vacuum-exhausted while closing the valve.

Abstract translation: 根据一个实施例，提供了一种激光离子源。 激光离子源包括真空室，真空排出，目标物被输送和固定，当靶被输送到真空室中时被打开并且除运输之外是关闭的阀;目标供给室， 保持能够移动的目标;以及运送单元，其在关闭所述阀的同时在所述目标供给室被真空排气之后，在打开所述阀的同时将保持在所述目标供给室上的所述目标物输送到所述真空室。