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    QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS USING OLIGONUCLEOTIDE MASS TAGS
    7.
    发明申请
    QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS USING OLIGONUCLEOTIDE MASS TAGS 审中-公开
    使用寡核苷酸质量标签对核酸和蛋白质进行定量

    公开(公告)号：US20140080126A1

    公开(公告)日：2014-03-20

    申请号：US13863839

    申请日：2013-04-16

    申请人： TRUSTEES OF BOSTON UNIVERSITY

    发明人： Charles R. Cantor ,  Lingang Zhang ,  Simon Kasif

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/6809 ,  C12Q2565/627 ,  C12Q2563/167 ,  C12Q2545/114

    摘要： The invention provides a method for detecting and quantifying the amount of target molecules, such as nucleic acids or proteins in a sample. The target molecules are first recognized and bounded by target-specific probes, generally nucleic acids or proteins that bind specifically to the targets, each of which is labeled with a short single-stranded nucleic acid probe, either DNA or RNA, with distinct molecular weight. This label is called an oligonucleotide mass tag. One or several standard oligonucleotide sequences can be designed with similar sequence but distinct molecular weight to those oligonucleotide mass tags. Then the oligonucleotide mass tags associated with bounded probes and the standard sequences are co-amplified using a pair of common primers. The presence and/or amount of each oligonucleotide mass tag, which corresponds to the amount of corresponding target molecule, is determined by a primer extension reaction and quantification of the primer extension product.

    摘要翻译： 本发明提供了用于检测和定量样品中靶分子如核酸或蛋白质的量的方法。 目标分子首先被目标特异性探针识别并限制，通常是与靶标特异性结合的核酸或蛋白质，其中每个标记有短单链核酸探针，DNA或RNA，具有不同的分子量 。 该标签称为寡核苷酸质量标签。 可以设计一个或几个标准寡核苷酸序列，其具有与那些寡核苷酸质量标签相似的序列但分子量不同。 然后使用一对普通引物共同扩增与有界探针和标准序列相关的寡核苷酸质粒标签。 通过引物延伸反应和引物延伸产物的定量来确定对应于相应靶分子量的每个寡核苷酸质粒标签的存在和/或量。

    Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis
    9.
    发明授权
    Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis 有权
    基于亲和选择器的识别和量化系统和方法在多个分析物中进行单一分析

    公开(公告)号：US08455202B2

    公开(公告)日：2013-06-04

    申请号：US12721173

    申请日：2010-03-10

    申请人： Fred E. Regnier ,  Nicholas B. Herold ,  Kevin W. Meyer

    发明人： Fred E. Regnier ,  Nicholas B. Herold ,  Kevin W. Meyer

    IPC分类号： G01N33/53

    CPC分类号： G01N33/53 ,  C12Q1/6816 ,  G01N33/6842 ,  G01N33/6848 ,  C12Q2525/107 ,  C12Q2537/143 ,  C12Q2563/167

    摘要： A multi-dimensional method for simultaneously analyzing multiple analytes within a sample solution, comprising adding affinity selectors to a sample solution containing analytes to be measured, the affinity selectors having an affinity for one or more of the analytes within the sample solution; allowing immune complexes to form between the affinity selectors and the analytes; partially or totally resolving the formed immune complexes from non-analyte substances within the sample solution; dissociating the resolved immune complexes; separating the analytes and the affinity selectors of the dissociated immune complexes from one another by capturing the analytes through a surface adsorption process; transferring the captured analytes to a detection means; and resolving the analytes with the detection means in accordance with their mass-to-charge ratios.

    摘要翻译： 一种用于同时分析样品溶液中的多种分析物的多维方法，包括将亲和选择剂添加到含有待测量待分析物的样品溶液中，所述亲和选择剂对样品溶液中的一种或多种分析物具有亲和力; 允许在亲和选择剂和分析物之间形成免疫复合物; 形成的免疫复合物部分或全部从样品溶液中的非分析物质中分离出来; 解离分离的免疫复合物; 通过表面吸附过程捕获分析物，将解离的免疫复合物的分析物和亲和选择剂彼此分离; 将捕获的分析物转移到检测装置; 并根据其质荷比，用检测装置分析分析物。