QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS USING OLIGONUCLEOTIDE MASS TAGS

    公开(公告)号:US20150184233A1

    公开(公告)日:2015-07-02

    申请号:US14558385

    申请日:2014-12-02

    IPC分类号: C12Q1/68

    摘要: The invention provides a method for detecting and quantifying the amount of target molecules, such as nucleic acids or proteins in a sample. The target molecules are first recognized and bounded by target-specific probes, generally nucleic acids or proteins that bind specifically to the targets, each of which is labeled with a short single-stranded nucleic acid probe, either DNA or RNA, with distinct molecular weight. This label is called an oligonucleotide mass tag. One or several standard oligonucleotide sequences can be designed with similar sequence but distinct molecular weight to those oligonucleotide mass tags. Then the oligonucleotide mass tags associated with bounded probes and the standard sequences are co-amplified using a pair of common primers. The presence and/or amount of each oligonucleotide mass tag, which corresponds to the amount of corresponding target molecule, is determined by a primer extension reaction and quantification of the primer extension product.

    QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS USING OLIGONUCLEOTIDE MASS TAGS
    2.
    发明申请
    QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS USING OLIGONUCLEOTIDE MASS TAGS 审中-公开
    使用寡核苷酸质量标签对核酸和蛋白质进行定量

    公开(公告)号:US20140080126A1

    公开(公告)日:2014-03-20

    申请号:US13863839

    申请日:2013-04-16

    IPC分类号: C12Q1/68

    摘要: The invention provides a method for detecting and quantifying the amount of target molecules, such as nucleic acids or proteins in a sample. The target molecules are first recognized and bounded by target-specific probes, generally nucleic acids or proteins that bind specifically to the targets, each of which is labeled with a short single-stranded nucleic acid probe, either DNA or RNA, with distinct molecular weight. This label is called an oligonucleotide mass tag. One or several standard oligonucleotide sequences can be designed with similar sequence but distinct molecular weight to those oligonucleotide mass tags. Then the oligonucleotide mass tags associated with bounded probes and the standard sequences are co-amplified using a pair of common primers. The presence and/or amount of each oligonucleotide mass tag, which corresponds to the amount of corresponding target molecule, is determined by a primer extension reaction and quantification of the primer extension product.

    摘要翻译: 本发明提供了用于检测和定量样品中靶分子如核酸或蛋白质的量的方法。 目标分子首先被目标特异性探针识别并限制,通常是与靶标特异性结合的核酸或蛋白质,其中每个标记有短单链核酸探针,DNA或RNA,具有不同的分子量 。 该标签称为寡核苷酸质量标签。 可以设计一个或几个标准寡核苷酸序列,其具有与那些寡核苷酸质量标签相似的序列但分子量不同。 然后使用一对普通引物共同扩增与有界探针和标准序列相关的寡核苷酸质粒标签。 通过引物延伸反应和引物延伸产物的定量来确定对应于相应靶分子量的每个寡核苷酸质粒标签的存在和/或量。