公开(公告)号：US20130271810A1

公开(公告)日：2013-10-17

申请号：US13857098

申请日：2013-04-04

Applicant: OLYMPUS CORPORATION

Inventor： Eiji YOKOI ,  Yasunari MATSUKAWA ,  Hiroshi SASAKI

IPC: G02B21/06 ,  G02F1/01

CPC classification number: G02B21/06 ,  G01N21/6458 ,  G02B21/16 ,  G02F1/01

Abstract: An illumination device includes a phase-modulation type spatial light modulator that is arranged at a position optically conjugate to a pupil position of an illumination lens between a light source and the illumination lens for irradiating light emitted from the light source on a sample, and a pupil projecting optics system for projecting at least one area within the pupil of the illumination lens onto a plurality of different areas of the spatial light modulator.

Abstract translation: 照明装置包括相位调制型空间光调制器，其被配置在与光源和照明透镜之间的照明透镜的光瞳位置光学共轭的位置处，用于将从光源发射的光照射在样本上， 光瞳投影光学系统，用于将照明透镜的光瞳内的至少一个区域投影到空间光调制器的多个不同区域上。

公开(公告)号：US20160109694A1

公开(公告)日：2016-04-21

申请号：US14880466

申请日：2015-10-12

Applicant: OLYMPUS CORPORATION

Inventor： Katsuyuki HASHIMOTO ,  Kunihiko SASAKI ,  Yasunari MATSUKAWA ,  Akinori ARAYA

IPC: G02B21/36 ,  G02B21/16 ,  G02B21/00 ,  G02B21/06

CPC classification number: G02B21/365 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/06 ,  G02B21/16

Abstract: With the object of expanding the dynamic range of an intensity signal and suppressing degradation of a light detecting portion, a laser scanning microscope of the present invention includes a light irradiating portion that irradiates a sample with laser light; a PMT that detects fluorescence from the sample and that outputs an intensity signal; a data processing portion that converts the intensity signal to brightness information at each pixel; a full-scale setting portion that can optionally set a normal full scale that is smaller than the intensity signal of the fluorescence from the sample when a light stimulus is given thereto and an expanded full scale that is larger than the normal full scale, as a maximum range of the intensity signal that can be converted to brightness information; and a control portion that determines whether to switch between a normal observation mode in which the brightness information of the sample when the light stimulus is not given thereto is obtained and a stimulation observation mode in which the brightness information of the sample when the light stimulus is given thereto is obtained; wherein the full-scale switching portion sets the normal full scale in the normal observation mode and sets the expanded full scale in the stimulation observation mode, on the basis of the result of the determination in the control portion.

Abstract translation: 为了扩大强度信号的动态范围并抑制光检测部的劣化，本发明的激光扫描显微镜包括用激光照射样品的光照射部， PMT，其检测来自样品的荧光并输出强度信号; 数据处理部分，其将强度信号转换为每个像素处的亮度信息; 满量程设置部分，当给予光刺激时，可以可选地设置小于来自样本的荧光的强度信号的正常满量程，以及大于正常满量程的扩展满量程，作为 能够转换为亮度信息的强度信号的最大范围; 以及控制部，其判定是否在不给予所述光刺激时的样本的亮度信息的正常观察模式之间切换，以及刺激观察模式，其中，当所述光刺激为亮时，所述样本的亮度信息为 获得给予; 其中，满量程切换部分基于正常观察模式中的正常满量程，并且根据控制部分中的确定结果在刺激观察模式中设定扩大满量程。

公开(公告)号：US20150371368A1

公开(公告)日：2015-12-24

申请号：US14728396

申请日：2015-06-02

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yusuke YAMASHITA ,  Yasunari MATSUKAWA

IPC: G06T3/40 ,  G02B21/00 ,  G06T7/00

CPC classification number: G06T3/4053 ,  G01N21/6458 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/367 ,  G02B27/58 ,  G06T7/0012 ,  G06T2207/10056

Abstract: A good super-resolution image can be generated, while eliminating a troublesome task necessary for generating a raw image. Provided is a sample observation apparatus including a memory, which stores a first number of photoelectrons required in a raw image for generating a super-resolution image, and a main controller, which calculates the number of image data sets to be added together for generating the raw image based on the first number of photoelectrons stored in the memory and a predetermined image acquisition condition, which acquires multiple sets of image data of the same region of a sample by repeatedly detecting light from the same region based on the calculated number of image data sets, and which generates the raw image by adding together the acquired multiple sets of image data of the same region.

Abstract translation: 可以生成良好的超分辨率图像，同时消除生成原始图像所需的麻烦任务。 提供一种样本观察装置，其包括存储器，其存储用于生成超分辨率图像的原始图像中所需的第一数量的光电子，以及主控制器，其计算要相加在一起的图像数据集的数量，以生成 基于存储在存储器中的第一数量的光电子的原始图像和预定图像获取条件，其基于计算的图像数据重复地检测来自相同区域的光来获取样本的相同区域的多组图像数据 并且通过将获取的相同区域的多组图像数据相加在一起而生成原始图像。

公开(公告)号：US20150177502A1

公开(公告)日：2015-06-25

申请号：US14580133

申请日：2014-12-22

Applicant: OLYMPUS CORPORATION

Inventor： Yasunari MATSUKAWA

IPC: G02B21/00 ,  G02F1/13 ,  G02F1/11 ,  G01N21/64 ,  G02F1/133

CPC classification number: G02B21/0032 ,  G01N21/6458 ,  G01N2021/6471 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16

Abstract: To avoid unstable light radiation during switching of a phase modulation amount and stimulate desired stimulation points simultaneously, provided is a photo-stimulator which includes an AOM switching on/off of radiation of stimulation light to a specimen S; an LCOS-SLM being capable of modulating a phase of stimulation light once radiation to the specimen S has been turned on by the AOM; and a controller controlling the LCOS-SLM to switch a phase modulation amount of stimulation light and controlling the AOM to switch on/off of radiation of stimulation light, wherein the controller causes the AOM to turn off radiation of stimulation light before the LCOS-SLM starts switching a phase modulation amount, and causes the AOM to turn on radiation of stimulation light after the LCOS-SLM completes switching a phase modulation amount.

Abstract translation: 为了在切换相位调制量期间避免不稳定的光辐射并且同时刺激所需的刺激点，提供了一种光刺激器，其包括AOM将刺激光的辐射切换到样本S; 能够通过AOM打开辐射至样本S的能够调制刺激光的相位的LCOS-SLM; 以及控制器，控制LCOS-SLM以切换刺激光的相位调制量并控制AOM以打开/关闭刺激光的辐射，其中控制器使AOM在LCOS-SLM之前关闭刺激光的辐射 开始切换相位调制量，并且在LCOS-SLM完成切换相位调制量之后使得AOM打开刺激光的辐射。

公开(公告)号：US20220082808A1

公开(公告)日：2022-03-17

申请号：US17534933

申请日：2021-11-24

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro YAMAZAKI ,  Yasunari MATSUKAWA

IPC: G02B21/24 ,  G02B21/36

Abstract: A method of adjusting an optical apparatus that acquires an image of a sample includes the steps of moving a focusing section included in the optical apparatus in a direction in which a contrast of a first image of the sample increases, and adjusting an aberration amount occurring in the optical apparatus in a direction in which a contrast of a second image of the sample increases.

公开(公告)号：US20180196248A1

公开(公告)日：2018-07-12

申请号：US15861333

申请日：2018-01-03

Applicant: OLYMPUS CORPORATION

Inventor： Hiroyuki YABUGAKI ,  Yasunari MATSUKAWA ,  Tetsuya SHIROTA

IPC: G02B21/36 ,  G06F3/041 ,  G02B21/24

Abstract: A microscope system includes: an microscope provided with electronic units; a display configured to display operation information; a touch panel configured to detect a position of contact of an object from outside; a setting unit configured to set areas corresponding respectively to multiple operation functions in a detection area of the touch panel; a pressure detector configured to detect a value of a pressure applied in the position of contact; and a drive controller configured to generate, based on the position of contact that is detected by the touch panel, the number of positions of contact, a change of the position of contact over time, and the value of the pressure detected by the pressure detector, an instruction signal of an instruction to change an observation status in which the specimen is observed with the electronic unit corresponding to operation function that is set by the setting unit.

公开(公告)号：US20150192766A1

公开(公告)日：2015-07-09

申请号：US14549283

申请日：2014-11-20

Applicant: OLYMPUS CORPORATION

Inventor： Yasunari MATSUKAWA

IPC: G02B21/06 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G02B21/0032 ,  G02B21/0076 ,  G02B2207/114

Abstract: The number of stimulated spots and/or the area of a stimulated spot is easily increased or decreased while keeping the irradiation power for each stimulated spot constant. Provided is a light stimulation device (3) that includes an objective lens (23) that radiates stimulation light emitted from a stimulation laser (11) onto a specimen (S); an LCOS-SLM (17) that is located at a position conjugate to the pupil of the objective lens (23) and that can modulate the phase of stimulation light to be made to enter the objective lens (23) based on a predetermined pupil modulation pattern; an AOM (13) that adjusts the amount of stimulation light to be made to enter the LCOS-SLM (17); a mouse (26) that is used to specify the number of spots and the position of a spot of light to be radiated onto the specimen (S); and a controller main unit (28) that determines the pupil modulation pattern for the LCOS-SLM (17) according to the number of spots and the position of a spot specified by means of the mouse (26) and that controls the AOM (13) such that, when the number of spots specified by means of the mouse (26) is changed, the amount of light to be radiated onto each spot before and after the change in the number of spots becomes constant.

Abstract translation: 刺激斑点的数量和/或刺激斑点的面积容易增加或减少，同时保持每个受激点的照射功率恒定。 提供了一种光刺激装置（3），其包括将从刺激激光器（11）发射的刺激光辐射到样本（S）上的物镜（23）; 位于与所述物镜（23）的光瞳共轭的位置的LCOS-SLM（17），并且能够基于预定的光瞳调制来调制待进入所述物镜（23）的刺激光的相位 模式; 调节进入LCOS-SLM（17）的刺激光量的AOM（13）; 用于指定要照射到样本（S）上的光点数和位置的鼠标（26）; 以及控制器主单元（28），其根据点数的数量和通过鼠标（26）指定的点的位置来确定LCOS-SLM（17）的光瞳调制模式，并且控制AOM（13 ），使得当通过鼠标（26）指定的点的数量改变时，要在点数变化之前和之后辐射到每个点上的光量变为恒定。