-
公开(公告)号:US20130306839A1
公开(公告)日:2013-11-21
申请号:US13895216
申请日:2013-05-15
Applicant: OLYMPUS CORPORATION
Inventor: Yohei KUWABARA , Akinori ARAYA , Kunihiko SASAKI
IPC: G01J1/46
CPC classification number: G01J1/46 , G02B21/002 , G02B21/008
Abstract: Provided is a photodetector including a light receiving portion that accumulates and outputs the amount of received observation light as electric charges; an offset portion that adds an offset signal to an output signal from the light receiving portion; an AD conversion portion that AD-converts the sum of the output signal and the offset signal obtained by the offset portion; a control unit that controls the AD conversion portion such that it A-D converts said sum when the light receiving portion is charged and discharged; and an offset correcting portion that corrects the offset signal by adjusting the value of the offset signal contained in the black level value such that the black level value obtained by the AD conversion portion from said sum when the capacitor is discharged becomes equal to a predetermined target value.
Abstract translation: 提供一种光电检测器,其包括:作为电荷累积并输出接收到的观察光量的光接收部; 偏移部分,其将偏移信号与来自所述光接收部分的输出信号相加; AD转换部分,AD转换由偏移部分获得的输出信号和偏移信号之和; 控制单元,其控制AD转换部分,使得当所述光接收部分被充电和放电时,其A-D转换所述和; 以及偏移校正部分,通过调整包含在黑电平值中的偏移信号的值来校正偏移信号,使得当电容器放电时由AD转换部分从所述和获得的黑电平值变为等于预定目标 值。
-
公开(公告)号:US20160109694A1
公开(公告)日:2016-04-21
申请号:US14880466
申请日:2015-10-12
Applicant: OLYMPUS CORPORATION
Inventor: Katsuyuki HASHIMOTO , Kunihiko SASAKI , Yasunari MATSUKAWA , Akinori ARAYA
CPC classification number: G02B21/365 , G02B21/002 , G02B21/0076 , G02B21/008 , G02B21/06 , G02B21/16
Abstract: With the object of expanding the dynamic range of an intensity signal and suppressing degradation of a light detecting portion, a laser scanning microscope of the present invention includes a light irradiating portion that irradiates a sample with laser light; a PMT that detects fluorescence from the sample and that outputs an intensity signal; a data processing portion that converts the intensity signal to brightness information at each pixel; a full-scale setting portion that can optionally set a normal full scale that is smaller than the intensity signal of the fluorescence from the sample when a light stimulus is given thereto and an expanded full scale that is larger than the normal full scale, as a maximum range of the intensity signal that can be converted to brightness information; and a control portion that determines whether to switch between a normal observation mode in which the brightness information of the sample when the light stimulus is not given thereto is obtained and a stimulation observation mode in which the brightness information of the sample when the light stimulus is given thereto is obtained; wherein the full-scale switching portion sets the normal full scale in the normal observation mode and sets the expanded full scale in the stimulation observation mode, on the basis of the result of the determination in the control portion.
Abstract translation: 为了扩大强度信号的动态范围并抑制光检测部的劣化,本发明的激光扫描显微镜包括用激光照射样品的光照射部, PMT,其检测来自样品的荧光并输出强度信号; 数据处理部分,其将强度信号转换为每个像素处的亮度信息; 满量程设置部分,当给予光刺激时,可以可选地设置小于来自样本的荧光的强度信号的正常满量程,以及大于正常满量程的扩展满量程,作为 能够转换为亮度信息的强度信号的最大范围; 以及控制部,其判定是否在不给予所述光刺激时的样本的亮度信息的正常观察模式之间切换,以及刺激观察模式,其中,当所述光刺激为亮时,所述样本的亮度信息为 获得给予; 其中,满量程切换部分基于正常观察模式中的正常满量程,并且根据控制部分中的确定结果在刺激观察模式中设定扩大满量程。
-