公开(公告)号：US20140285654A1

公开(公告)日：2014-09-25

申请号：US14217028

申请日：2014-03-17

Applicant: OLYMPUS CORPORATION

Inventor： Akinori ARAYA ,  Yohei KUWABARA

IPC: G02B21/00

CPC classification number: G02B21/008 ,  G02B21/0076

Abstract: Brightness information with a wide dynamic range is acquired and observed while preventing degradation of a detector. The invention provides a microscope system including a scanner that two-dimensionally scans laser light emitted from a light source unit on a specimen; a detector (41) that detects light from the specimen on which the laser light is scanned by the scanner and outputs a light intensity signal corresponding to the light intensity of the detected light; a PC that converts the light intensity signal output from the detector (41) to brightness information at each pixel corresponding to the scanning position of the scanner and that generates an image of the specimen; a second integrating circuit (70) that calculates an integrated value corresponding to the total light intensity signal output from the detector (41) within a given time; a comparator (55) that determines whether or not that integrated value of a voltage signal exceeds a prescribed threshold; and a high-voltage control portion (87) that stops detection of light by the detector (41) when it is determined by the comparator (55) that the voltage signal exceeds the prescribed threshold.

Abstract translation: 获取和观察具有宽动态范围的亮度信息，同时防止检测器的劣化。 本发明提供了一种显微镜系统，包括：二维地扫描从样本上的光源单元发射的激光的扫描仪; 检测器（41），其检测来自所述检体的来自所述扫描仪的扫描仪的激光的光，并输出与所检测的光的光强对应的光强度信号; 将从检测器（41）输出的光强度信号转换成与扫描仪的扫描位置对应的各像素的亮度信息并生成样本的图像的PC; 第二积分电路（70），其在给定时间内计算与从所述检测器（41）输出的总光强度信号对应的积分值; 确定电压信号的积分值是否超过规定阈值的比较器（55）; 以及当由比较器（55）确定电压信号超过规定阈值时，由检测器（41）停止检测光的高压控制部（87）。

公开(公告)号：US20130306839A1

公开(公告)日：2013-11-21

申请号：US13895216

申请日：2013-05-15

Applicant: OLYMPUS CORPORATION

Inventor： Yohei KUWABARA ,  Akinori ARAYA ,  Kunihiko SASAKI

IPC: G01J1/46

CPC classification number: G01J1/46 ,  G02B21/002 ,  G02B21/008

Abstract: Provided is a photodetector including a light receiving portion that accumulates and outputs the amount of received observation light as electric charges; an offset portion that adds an offset signal to an output signal from the light receiving portion; an AD conversion portion that AD-converts the sum of the output signal and the offset signal obtained by the offset portion; a control unit that controls the AD conversion portion such that it A-D converts said sum when the light receiving portion is charged and discharged; and an offset correcting portion that corrects the offset signal by adjusting the value of the offset signal contained in the black level value such that the black level value obtained by the AD conversion portion from said sum when the capacitor is discharged becomes equal to a predetermined target value.

Abstract translation: 提供一种光电检测器，其包括：作为电荷累积并输出接收到的观察光量的光接收部; 偏移部分，其将偏移信号与来自所述光接收部分的输出信号相加; AD转换部分，AD转换由偏移部分获得的输出信号和偏移信号之和; 控制单元，其控制AD转换部分，使得当所述光接收部分被充电和放电时，其A-D转换所述和; 以及偏移校正部分，通过调整包含在黑电平值中的偏移信号的值来校正偏移信号，使得当电容器放电时由AD转换部分从所述和获得的黑电平值变为等于预定目标 值。

公开(公告)号：US20180167561A1

公开(公告)日：2018-06-14

申请号：US15793535

申请日：2017-10-25

Applicant: OLYMPUS CORPORATION

Inventor： Akinori ARAYA

IPC: H04N5/243 ,  H04N5/235 ,  G02B21/36 ,  H04N5/378

Abstract: Observation in which the properties of a detection element are utilized is performed. Provided is a detection device including: a detector that has a linear response characteristic, in which an output signal changes linearly, and a nonlinear response characteristic, in which the output signal changes nonlinearly, and that detects light from a sample and outputs the output signal in accordance with the intensity of the light; a light detection circuit that is capable of switching between a first amplification factor and a second amplification factor, the light detection circuit amplifying the output signal output from the detector based on the first amplification factor or the second amplification factor so as to generate a brightness signal; and an input unit with which a user is allowed to switch the amplification factor for the output signal to be used between the first amplification factor and the second amplification factor.

公开(公告)号：US20160109694A1

公开(公告)日：2016-04-21

申请号：US14880466

申请日：2015-10-12

Applicant: OLYMPUS CORPORATION

Inventor： Katsuyuki HASHIMOTO ,  Kunihiko SASAKI ,  Yasunari MATSUKAWA ,  Akinori ARAYA

IPC: G02B21/36 ,  G02B21/16 ,  G02B21/00 ,  G02B21/06

CPC classification number: G02B21/365 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/06 ,  G02B21/16

Abstract: With the object of expanding the dynamic range of an intensity signal and suppressing degradation of a light detecting portion, a laser scanning microscope of the present invention includes a light irradiating portion that irradiates a sample with laser light; a PMT that detects fluorescence from the sample and that outputs an intensity signal; a data processing portion that converts the intensity signal to brightness information at each pixel; a full-scale setting portion that can optionally set a normal full scale that is smaller than the intensity signal of the fluorescence from the sample when a light stimulus is given thereto and an expanded full scale that is larger than the normal full scale, as a maximum range of the intensity signal that can be converted to brightness information; and a control portion that determines whether to switch between a normal observation mode in which the brightness information of the sample when the light stimulus is not given thereto is obtained and a stimulation observation mode in which the brightness information of the sample when the light stimulus is given thereto is obtained; wherein the full-scale switching portion sets the normal full scale in the normal observation mode and sets the expanded full scale in the stimulation observation mode, on the basis of the result of the determination in the control portion.

Abstract translation: 为了扩大强度信号的动态范围并抑制光检测部的劣化，本发明的激光扫描显微镜包括用激光照射样品的光照射部， PMT，其检测来自样品的荧光并输出强度信号; 数据处理部分，其将强度信号转换为每个像素处的亮度信息; 满量程设置部分，当给予光刺激时，可以可选地设置小于来自样本的荧光的强度信号的正常满量程，以及大于正常满量程的扩展满量程，作为 能够转换为亮度信息的强度信号的最大范围; 以及控制部，其判定是否在不给予所述光刺激时的样本的亮度信息的正常观察模式之间切换，以及刺激观察模式，其中，当所述光刺激为亮时，所述样本的亮度信息为 获得给予; 其中，满量程切换部分基于正常观察模式中的正常满量程，并且根据控制部分中的确定结果在刺激观察模式中设定扩大满量程。