公开(公告)号：US20160109694A1

公开(公告)日：2016-04-21

申请号：US14880466

申请日：2015-10-12

Applicant: OLYMPUS CORPORATION

Inventor： Katsuyuki HASHIMOTO ,  Kunihiko SASAKI ,  Yasunari MATSUKAWA ,  Akinori ARAYA

IPC: G02B21/36 ,  G02B21/16 ,  G02B21/00 ,  G02B21/06

CPC classification number: G02B21/365 ,  G02B21/002 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/06 ,  G02B21/16

Abstract: With the object of expanding the dynamic range of an intensity signal and suppressing degradation of a light detecting portion, a laser scanning microscope of the present invention includes a light irradiating portion that irradiates a sample with laser light; a PMT that detects fluorescence from the sample and that outputs an intensity signal; a data processing portion that converts the intensity signal to brightness information at each pixel; a full-scale setting portion that can optionally set a normal full scale that is smaller than the intensity signal of the fluorescence from the sample when a light stimulus is given thereto and an expanded full scale that is larger than the normal full scale, as a maximum range of the intensity signal that can be converted to brightness information; and a control portion that determines whether to switch between a normal observation mode in which the brightness information of the sample when the light stimulus is not given thereto is obtained and a stimulation observation mode in which the brightness information of the sample when the light stimulus is given thereto is obtained; wherein the full-scale switching portion sets the normal full scale in the normal observation mode and sets the expanded full scale in the stimulation observation mode, on the basis of the result of the determination in the control portion.

Abstract translation: 为了扩大强度信号的动态范围并抑制光检测部的劣化，本发明的激光扫描显微镜包括用激光照射样品的光照射部， PMT，其检测来自样品的荧光并输出强度信号; 数据处理部分，其将强度信号转换为每个像素处的亮度信息; 满量程设置部分，当给予光刺激时，可以可选地设置小于来自样本的荧光的强度信号的正常满量程，以及大于正常满量程的扩展满量程，作为 能够转换为亮度信息的强度信号的最大范围; 以及控制部，其判定是否在不给予所述光刺激时的样本的亮度信息的正常观察模式之间切换，以及刺激观察模式，其中，当所述光刺激为亮时，所述样本的亮度信息为 获得给予; 其中，满量程切换部分基于正常观察模式中的正常满量程，并且根据控制部分中的确定结果在刺激观察模式中设定扩大满量程。

公开(公告)号：US20190187448A1

公开(公告)日：2019-06-20

申请号：US16222396

申请日：2018-12-17

Applicant: OLYMPUS CORPORATION

Inventor： Katsuyuki HASHIMOTO

IPC: G02B21/00

CPC classification number: G02B21/0048 ,  G02B21/0032 ,  G02B21/008

Abstract: A scanning laser microscope includes: an objective lens; an XY scanner; a Z scanner; a detector that detects fluorescence from a sample and that outputs luminance information; an image processor that generates an image based on the luminance information and scanning-position information; and a controller that controls these components, wherein while the controller shifts the scanning line by the XY scanner each time the controller scans one line by the XY scanner, the controller changes the scanning position in forward and return paths of the direction along the optical axis of the objective lens by the Z scanner, reverses, between the forward and return path, an orientation in which the scanning line is shifted by the XY scanner, and reverses, about an axial line along the main scanning direction, either the image of the plane in the forward path or the image of the plane in the return path.

公开(公告)号：US20170102531A1

公开(公告)日：2017-04-13

申请号：US15258677

申请日：2016-09-07

Applicant: OLYMPUS CORPORATION

Inventor： Katsuyuki HASHIMOTO

IPC: G02B21/00 ,  G02B26/10

CPC classification number: G02B21/0048 ,  G02B26/105

Abstract: Provided is a laser scanning microscope including a scanning unit that scans laser light emitted from a light source two-dimensionally across a specimen, a light-detecting unit that detects light coming from the specimen, an information-acquiring unit that acquires information about an observation mode, and a control unit that controls the scanning unit based on the information acquired by the information-acquiring unit. The scanning unit includes a galvanometer scanner and a resonant scanner that are alternately operable. The control unit controls the scanning unit to deactivate the resonant scanner if the information acquired by the information-acquiring unit indicates an observation mode in which the galvanometer scanner is used alone and to maintain the resonant scanner in an active state if the information acquired by the information-acquiring unit indicates an observation mode in which the galvanometer scanner and the resonant scanner are alternately used.