公开(公告)号：US20160363748A1

公开(公告)日：2016-12-15

申请号：US15161997

申请日：2016-05-23

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yoshihiro SHIMADA ,  Kosuke TAKAGI

IPC: G02B21/00 ,  G02B21/26

CPC classification number: G02B21/006 ,  G02B21/0028 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/367

Abstract: Provided is a microscope system including a motor-driven stage on which is mounted a culture vessel containing one or more cell clusters, each including cells having a target molecule labeled with a fluorescent or luminescent chemical; a low-magnification-image acquiring unit that acquires low-magnification images of the cell clusters in the culture vessel mounted on the stage; a detecting unit that detects the position of each cell cluster in the culture vessel by analyzing the acquired low-magnification images; and a high-magnification-image acquiring unit that, after the detected position is aligned with the optical axis of an objective lens, acquires slice images of fluorescence or luminescence emitted from the cells forming the cell cluster at a higher magnification than the low-magnification-image acquiring unit at intervals along the optical axis while the stage and/or the lens is moved to change stepwise the distance between the lens and the cell cluster.

Abstract translation: 提供了一种显微镜系统，其包括电动驱动平台，其上安装有包含一个或多个细胞簇的培养容器，每个细胞簇包括具有用荧光或发光化学品标记的靶分子的细胞; 低放大图像获取单元，其获取安装在所述台上的培养容器中的细胞簇的低倍率图像; 检测单元，其通过分析所获取的低倍率图像来检测培养容器中的每个细胞簇的位置; 以及高倍率图像获取单元，其在检测到的位置与物镜的光轴对准之后，以比低倍率更高的倍率获取从形成细胞簇的细胞发射的荧光或发光的切片图像 - 图像获取单元，沿着光轴间隔，同时移动台和/或透镜以逐步改变透镜和细胞簇之间的距离。

公开(公告)号：US20150205086A1

公开(公告)日：2015-07-23

申请号：US14560963

申请日：2014-12-04

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA

IPC: G02B21/00 ,  G02B21/16 ,  G01N21/64

CPC classification number: G02B21/002 ,  G01N21/6458 ,  G01N2021/6419 ,  G02B21/0032 ,  G02B21/0064 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/16

Abstract: An image having an expected superresolution effect is created in a straightforward manner and with superior precision. The invention provides a scanning laser microscope including a scanner that scans a laser beam emitted from an Ar laser device on a specimen; an objective lens that radiates the laser beam scanned by the scanner onto the specimen and that collects return light coming from the specimen; a detector array that has a plurality of minute detector elements arrayed at a position that is optically conjugate with the focal position of the objective lens; and a superresolution calculating portion that calculates a center position of a spot of the return light that is incident on the detector array on the basis of a light intensity signal output from each of the minute detector elements in the detector array.

Abstract translation: 具有预期的超分辨率效果的图像以直接的方式和优异的精度被创建。 本发明提供了一种扫描激光显微镜，其包括扫描仪，其扫描从Ar激光装置发射的激光束在样本上; 将由扫描仪扫描的激光束辐射到样品上并收集来自样品的返回光的物镜; 检测器阵列，其具有排列在与物镜的焦点位置光学共轭的位置处的多个微小检测器元件; 以及超分辨率计算部，其基于从检测器阵列中的每个微小检测器元件输出的光强度信号来计算入射在检测器阵列上的返回光的光点的中心位置。

公开(公告)号：US20190121112A1

公开(公告)日：2019-04-25

申请号：US16160244

申请日：2018-10-15

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yusuke YAMASHITA

IPC: G02B21/36 ,  G06T11/20 ,  G06K9/00 ,  G06T15/08 ,  G06T7/73

Abstract: Visual observation of morphological features of a cell group or individual cells acquired in 3D image data is facilitated, thus improving observation accuracy. Provided is an image processing device that generates, on the basis of a plurality of 2D images acquired by a microscope at different focus positions on a cell clump, 3D images of respective cells constituting the cell clump, that processes the generated 3D images and analyzes feature amounts on the basis of at least one measurement parameter, that displays analysis results in a graph, that allows a user to select a region of interest on the displayed graph, and that generates, from the 3D images that correspond to the plurality of cells that are included in the selected region of interest, 2D display images each in a plane with reference to an axis that is determined on the basis of a shape feature of the corresponding cell and displays the 2D display images in a list.

公开(公告)号：US20150371368A1

公开(公告)日：2015-12-24

申请号：US14728396

申请日：2015-06-02

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yusuke YAMASHITA ,  Yasunari MATSUKAWA

IPC: G06T3/40 ,  G02B21/00 ,  G06T7/00

CPC classification number: G06T3/4053 ,  G01N21/6458 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/367 ,  G02B27/58 ,  G06T7/0012 ,  G06T2207/10056

Abstract: A good super-resolution image can be generated, while eliminating a troublesome task necessary for generating a raw image. Provided is a sample observation apparatus including a memory, which stores a first number of photoelectrons required in a raw image for generating a super-resolution image, and a main controller, which calculates the number of image data sets to be added together for generating the raw image based on the first number of photoelectrons stored in the memory and a predetermined image acquisition condition, which acquires multiple sets of image data of the same region of a sample by repeatedly detecting light from the same region based on the calculated number of image data sets, and which generates the raw image by adding together the acquired multiple sets of image data of the same region.

Abstract translation: 可以生成良好的超分辨率图像，同时消除生成原始图像所需的麻烦任务。 提供一种样本观察装置，其包括存储器，其存储用于生成超分辨率图像的原始图像中所需的第一数量的光电子，以及主控制器，其计算要相加在一起的图像数据集的数量，以生成 基于存储在存储器中的第一数量的光电子的原始图像和预定图像获取条件，其基于计算的图像数据重复地检测来自相同区域的光来获取样本的相同区域的多组图像数据 并且通过将获取的相同区域的多组图像数据相加在一起而生成原始图像。

公开(公告)号：US20150316757A1

公开(公告)日：2015-11-05

申请号：US14694555

申请日：2015-04-23

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA

IPC: G02B21/00 ,  H04N5/225 ,  G02B21/36

CPC classification number: G02B21/0076 ,  G01N21/6458 ,  G02B21/0044 ,  G02B21/0048 ,  G02B21/008 ,  G02B21/361 ,  G02B21/367 ,  G02B27/58 ,  H04N5/2254

Abstract: A superior superresolution image can be generated. Provided is a specimen observation apparatus including an objective lens that radiates excitation laser light emitted from a light source onto a specimen; and a main controller that obtains a plurality of sets of image data of the same region on the specimen by repeatedly detecting fluorescence from the same region on the specimen irradiated with the excitation laser light by the objective lens, and that emphasizes high-frequency components in an addition image data obtained by adding the obtained plurality of sets of image data of the same region on the specimen.

Abstract translation: 可以生成优异的超分辨率图像。 本发明提供一种试样观察装置，其具备将从光源射出的激发激光照射在试样上的物镜; 以及主控制器，通过重复地检测通过物镜照射激发激光的样本上的相同区域的荧光，获得样本上相同区域的多组图像数据，并且强调高频分量 通过将所获得的多组相同区域的图像数据组合在样本上而获得的加法图像数据。