公开(公告)号：US20160363749A1

公开(公告)日：2016-12-15

申请号：US15162188

申请日：2016-05-23

Applicant: OLYMPUS CORPORATION

Inventor： Yusuke YAMASHITA

IPC: G02B21/00 ,  G02B27/10 ,  G02B21/36

Abstract: The purpose of the present invention is to detect a fixed number of photons or greater in a short time and to create a super-resolution image having a desired resolution equal to optical resolution or higher. A microscope system includes: a scanner that scans laser light emitted from a continuous-wave light source on a specimen; a beam splitter that splits fluorescence from the specimen on which the laser light is scanned into a plurality of light paths with the same wavelength; a plurality of PMTs that respectively detect the fluorescence in the light paths split by the beam splitter and output light intensity signals; a PC that, each time the laser light is repeatedly scanned by the scanner, for each of the PMTs, acquires an image dataset of the specimen on the basis of the light intensity signals, that combines the plurality of image datasets for the same area of the specimen, which are acquired on the basis of the light intensity signals of the fluorescence from the same area of the specimen, detected by each PMT, and that subjects this final combined image dataset to computational processing for enhancing high-frequency components.

Abstract translation: 本发明的目的是在短时间内检测固定数量的光子或更大的光子，并产生具有等于或更高的光学分辨率的期望分辨率的超分辨率图像。 显微镜系统包括：扫描仪，用于扫描来自连续波光源的样品上的激光; 分束器，其将来自所述激光的所述样本的荧光分成具有相同波长的多个光路; 多个PMT，分别检测由分束器分离的光路中的荧光并输出光强度信号; PC，每当PMT反复扫描激光时，根据光强度信号获取样本的图像数据集，所述光强度信号组合多个相同区域的图像数据集 根据每个PMT检测的来自样本的相同区域的荧光的光强度信号获取样本，并将该最终组合图像数据集合到用于增强高频分量的计算处理中。

公开(公告)号：US20150092265A1

公开(公告)日：2015-04-02

申请号：US14502540

申请日：2014-09-30

Applicant: OLYMPUS CORPORATION

Inventor： Yusuke YAMASHITA

IPC: G02B21/02

CPC classification number: G02B21/0032 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/0088 ,  G02B21/24 ,  G02B21/26

Abstract: A desired observation timing is ensured, and simultaneity of observation among different observation positions is ensured. Provided is a microscope system including an electrically powered stage, a scanner, objective lenses, a revolver, an image-acquisition portion, an autofocus portion, a first storage portion that stores one of the focal positions of the low-magnification objective lens as a reference position, a focal-position setting portion that sets a focal position at which an image is acquired with reference to the reference position for the low-magnification objective lens, an acquisition-position setting portion that sets acquisition positions for partial images, and a map-image generating portion that generates a map image based on the partial images acquired at the set focal position, wherein the autofocus portion detects a focal position of the high-magnification objective lens at an observation position set on the map image with reference to the reference position for the low-magnification objective lens.

Abstract translation: 确保期望的观察时间，并且确保不同观察位置之间的观察同时性。 提供了一种显微镜系统，包括电动台，扫描器，物镜，左轮手枪，图像采集部分，自动对焦部分，第一存储部分，其将低倍率物镜的焦点位置之一存储为 参考位置，参考用于低倍率物镜的基准位置来设置获取图像的焦点位置的焦点位置设置部分，设置用于部分图像的获取位置的获取位置设置部分，以及 地图图像生成部，其基于在所设置的焦点位置获取的部分图像生成地图图像，其中，所述自动聚焦部分参考所述地图图像在所述地图图像上设置的观察位置处检测所述高倍率物镜的焦点位置 低倍率物镜的基准位置。

公开(公告)号：US20190107528A1

公开(公告)日：2019-04-11

申请号：US16150753

申请日：2018-10-03

Applicant: OLYMPUS CORPORATION

Inventor： Yusuke YAMASHITA

IPC: G01N33/483 ,  G06T7/66 ,  G02B21/36 ,  G06T7/00

Abstract: A fixed region at an outer periphery or an inner periphery of a sample that has a three-dimensional structure is selectively analyzed with accuracy. Provided is an observation system including a CPU that recognizes the 3D shape of an observation target, such as a spheroid, from a 3D image of cells, that sets a 3D mask of which the radial distance from a circumscribed surface of the recognized 3D shape is fixed over the entire region of the circumscribed surface and of which the shape is similar to the circumscribed surface, and that identifies a cell contained inside the set 3D mask.

公开(公告)号：US20190121112A1

公开(公告)日：2019-04-25

申请号：US16160244

申请日：2018-10-15

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yusuke YAMASHITA

IPC: G02B21/36 ,  G06T11/20 ,  G06K9/00 ,  G06T15/08 ,  G06T7/73

Abstract: Visual observation of morphological features of a cell group or individual cells acquired in 3D image data is facilitated, thus improving observation accuracy. Provided is an image processing device that generates, on the basis of a plurality of 2D images acquired by a microscope at different focus positions on a cell clump, 3D images of respective cells constituting the cell clump, that processes the generated 3D images and analyzes feature amounts on the basis of at least one measurement parameter, that displays analysis results in a graph, that allows a user to select a region of interest on the displayed graph, and that generates, from the 3D images that correspond to the plurality of cells that are included in the selected region of interest, 2D display images each in a plane with reference to an axis that is determined on the basis of a shape feature of the corresponding cell and displays the 2D display images in a list.

公开(公告)号：US20180204047A1

公开(公告)日：2018-07-19

申请号：US15844441

申请日：2017-12-15

Applicant: OLYMPUS CORPORATION

Inventor： Yusuke YAMASHITA

IPC: G06K9/00 ,  G06K9/46 ,  G06K9/20 ,  G06T7/55

CPC classification number: G06K9/00147 ,  C12M1/34 ,  C12M41/36 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/00201 ,  G06K9/2081 ,  G06K9/469 ,  G06K2209/27 ,  G06T7/55 ,  G06T2207/10056 ,  G06T2207/10064

Abstract: Provided is an observation system including: a monitor; a CPU that identifies, in a 3D image including the plurality of cells, the respective cells by assigning labels that differ from one another to the respective cells, that associates three mutually-intersecting cross-sectional images that constitute the 3D image, and that simultaneously displays the cross-sectional images on the monitor; and an input unit with which an operator specifies an arbitrary cell in any of the cross-sectional images displayed on the monitor. The CPU extracts, from the 3D image, the cross-sectional shapes, in the respective cross-sectional images, of the cell specified by using the input unit, on the basis of the labels, associates the extracted cross-sectional shapes of the cell with one another, and displays the extracted cross-sectional shapes in the respective cross-sectional images displayed on the monitor, in a distinguishable manner from the other cells.

公开(公告)号：US20150371368A1

公开(公告)日：2015-12-24

申请号：US14728396

申请日：2015-06-02

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yusuke YAMASHITA ,  Yasunari MATSUKAWA

IPC: G06T3/40 ,  G02B21/00 ,  G06T7/00

CPC classification number: G06T3/4053 ,  G01N21/6458 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/367 ,  G02B27/58 ,  G06T7/0012 ,  G06T2207/10056

Abstract: A good super-resolution image can be generated, while eliminating a troublesome task necessary for generating a raw image. Provided is a sample observation apparatus including a memory, which stores a first number of photoelectrons required in a raw image for generating a super-resolution image, and a main controller, which calculates the number of image data sets to be added together for generating the raw image based on the first number of photoelectrons stored in the memory and a predetermined image acquisition condition, which acquires multiple sets of image data of the same region of a sample by repeatedly detecting light from the same region based on the calculated number of image data sets, and which generates the raw image by adding together the acquired multiple sets of image data of the same region.

Abstract translation: 可以生成良好的超分辨率图像，同时消除生成原始图像所需的麻烦任务。 提供一种样本观察装置，其包括存储器，其存储用于生成超分辨率图像的原始图像中所需的第一数量的光电子，以及主控制器，其计算要相加在一起的图像数据集的数量，以生成 基于存储在存储器中的第一数量的光电子的原始图像和预定图像获取条件，其基于计算的图像数据重复地检测来自相同区域的光来获取样本的相同区域的多组图像数据 并且通过将获取的相同区域的多组图像数据相加在一起而生成原始图像。