公开(公告)号：US20150153557A1

公开(公告)日：2015-06-04

申请号：US14559611

申请日：2014-12-03

Applicant: OLYMPUS CORPORATION

Inventor： Kosuke TAKAGI

IPC: G02B21/36 ,  G02B21/06 ,  G02B21/16

CPC classification number: G02B21/365 ,  G02B21/06 ,  G02B21/16

Abstract: A plurality of specimens or a plurality of targets in a specimen are accurately examined. A microscope apparatus includes a light source; an image-generating unit generating an image of a specimen; a control unit controlling the light source and the image-generating unit depending on a predetermined irradiation condition or a predetermined image generation condition; an image-analyzing unit analyzing the image generated by the image-generating unit to extract targets; and a condition-changing unit changing the irradiation condition and/or the image generation condition based on a difference between an actual luminance of each of the extracted targets in the image and a desired luminance such that the actual luminance of the target in the image satisfies the desired luminance. If the irradiation condition and/or the image generation condition is changed, the control unit redrives the light source and the image-generating unit depending on the changed irradiation condition and/or image generation condition.

Abstract translation: 准确地检查样本中的多个标本或多个靶。 显微镜装置包括光源; 产生样本的图像的图像生成单元; 控制单元，其根据预定的照射条件或预定的图像生成条件控制光源和图像生成单元; 图像分析单元，分析由图像生成单元生成的图像以提取目标; 以及条件改变单元，基于图像中的每个提取的目标的实际亮度与期望亮度之间的差异来改变照射条件和/或图像生成条件，使得图像中的目标的实际亮度满足 所需亮度。 如果照射条件和/或图像生成条件改变，则控制单元根据改变的照射条件和/或图像生成条件来重新驱动光源和图像生成单元。

公开(公告)号：US20160363748A1

公开(公告)日：2016-12-15

申请号：US15161997

申请日：2016-05-23

Applicant: OLYMPUS CORPORATION

Inventor： Hisao KITAGAWA ,  Yoshihiro SHIMADA ,  Kosuke TAKAGI

IPC: G02B21/00 ,  G02B21/26

CPC classification number: G02B21/006 ,  G02B21/0028 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/367

Abstract: Provided is a microscope system including a motor-driven stage on which is mounted a culture vessel containing one or more cell clusters, each including cells having a target molecule labeled with a fluorescent or luminescent chemical; a low-magnification-image acquiring unit that acquires low-magnification images of the cell clusters in the culture vessel mounted on the stage; a detecting unit that detects the position of each cell cluster in the culture vessel by analyzing the acquired low-magnification images; and a high-magnification-image acquiring unit that, after the detected position is aligned with the optical axis of an objective lens, acquires slice images of fluorescence or luminescence emitted from the cells forming the cell cluster at a higher magnification than the low-magnification-image acquiring unit at intervals along the optical axis while the stage and/or the lens is moved to change stepwise the distance between the lens and the cell cluster.

Abstract translation: 提供了一种显微镜系统，其包括电动驱动平台，其上安装有包含一个或多个细胞簇的培养容器，每个细胞簇包括具有用荧光或发光化学品标记的靶分子的细胞; 低放大图像获取单元，其获取安装在所述台上的培养容器中的细胞簇的低倍率图像; 检测单元，其通过分析所获取的低倍率图像来检测培养容器中的每个细胞簇的位置; 以及高倍率图像获取单元，其在检测到的位置与物镜的光轴对准之后，以比低倍率更高的倍率获取从形成细胞簇的细胞发射的荧光或发光的切片图像 - 图像获取单元，沿着光轴间隔，同时移动台和/或透镜以逐步改变透镜和细胞簇之间的距离。

公开(公告)号：US20160350915A1

公开(公告)日：2016-12-01

申请号：US15166195

申请日：2016-05-26

Applicant: OLYMPUS CORPORATION

Inventor： Kosuke TAKAGI ,  Yoshihiro SHIMADA

IPC: G06T7/00 ,  G06T7/60 ,  G06K9/52 ,  G01N33/483 ,  G06K9/62

CPC classification number: G06T7/0012 ,  G01N33/4833 ,  G06K9/0014 ,  G06K9/52 ,  G06T7/66 ,  G06T7/70 ,  G06T2200/04 ,  G06T2207/30004 ,  G06T2207/30024

Abstract: A cell evaluation method includes: a labeling step of labeling at least some of cells constituting a cell clump with a chemical substance; an image-capturing step of acquiring a plurality of slice images of the labeled cells, for at least part of the cell clump; an evaluating step of evaluating the characteristics of the cells on the basis of the plurality of slice images; a 3D-image creating step of creating a 3D image of at least part of the cell clump by subjecting the plurality of slice images to image processing; a center-of-gravity determining step of determining, from the 3D image, a center-of-gravity position of the cell clump; and an organizing step of organizing evaluation results obtained in the evaluating step, with respect to the distance from the center-of-gravity position.

Abstract translation: 细胞评价方法包括：标记步骤，用化学物质标记构成细胞团的至少一些细胞; 对于所述细胞团的至少一部分，获取所述标记细胞的多个切片图像的图像捕获步骤; 评估步骤，基于所述多个切片图像来评估所述单元的特性; 3D图像创建步骤，通过对所述多个切片图像进行图像处理来创建所述细胞团的至少一部分的3D图像; 重心确定步骤，从3D图像确定细胞团的重心位置; 以及组织步骤，用于组织在评估步骤中获得的评估结果相对于距离重心位置的距离。

公开(公告)号：US20190145959A1

公开(公告)日：2019-05-16

申请号：US16181782

申请日：2018-11-06

Applicant: OLYMPUS CORPORATION

Inventor： Mayu OGAWA ,  Kazuhito GODA ,  Kosuke TAKAGI

IPC: G01N33/50 ,  G01N21/64

Abstract: The present invention provides a method for evaluating a protrusion-forming ability of cell spheroids, comprising step (a) of imaging a cell spheroid labeled with a fluorescent substance using a fluorescence microscope at a resolution capable of identifying individual cells, and acquiring a plane tomographic image of a fluorescence emitted from the fluorescent substance, step (b) of analyzing the plane tomographic image acquired in step (a) to determine a protrusion part of the cell spheroid, and step (c) of evaluating a protrusion-forming ability of the cell spheroid based on the protrusion part determined in step (b).