公开(公告)号：US12100482B2

公开(公告)日：2024-09-24

申请号：US18503392

申请日：2023-11-07

申请人： GUARDANT HEALTH, INC.

发明人： Darya Chudova ,  Helmy Eltoukhy ,  Stefanie Ann Ward Mortimer ,  Diana Abdueva

IPC分类号： G16B30/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  G16B5/00 ,  G16B25/10 ,  G16B40/30

CPC分类号： G16B30/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  G16B5/00 ,  G16B25/10 ,  G16B40/30 ,  C12Q2535/122 ,  C12Q2537/159

摘要： The present disclosure provides a method for enriching for multiple genomic regions using a first bait set that selectively hybridizes to a first set of genomic regions of a nucleic acid sample and a second bait set that selectively hybridizes to a second set of genomic regions of the nucleic acid sample. These bait set panels can selectively enrich for one or more nucleosome-associated regions of a genome, said nucleosome-associated regions comprising genomic regions having one or more genomic base positions with differential nucleosomal occupancy, wherein the differential nucleosomal occupancy is characteristic of a cell or tissue type of origin or disease state.

公开(公告)号：US12094573B2

公开(公告)日：2024-09-17

申请号：US18506734

申请日：2023-11-10

申请人： GUARDANT HEALTH, INC.

发明人： Darya Chudova ,  Helmy Eltoukhy ,  Stefanie Ann Ward Mortimer ,  Diana Abdueva

IPC分类号： G16B30/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  G16B5/00 ,  G16B25/10 ,  G16B40/30

CPC分类号： G16B30/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  G16B5/00 ,  G16B25/10 ,  G16B40/30 ,  C12Q2535/122 ,  C12Q2537/159

摘要： The present disclosure provides a method for enriching for multiple genomic regions using a first bait set that selectively hybridizes to a first set of genomic regions of a nucleic acid sample and a second bait set that selectively hybridizes to a second set of genomic regions of the nucleic acid sample. These bait set panels can selectively enrich for one or more nucleosome-associated regions of a genome, said nucleosome-associated regions comprising genomic regions having one or more genomic base positions with differential nucleosomal occupancy, wherein the differential nucleosomal occupancy is characteristic of a cell or tissue type of origin or disease state.

公开(公告)号：US12077822B2

公开(公告)日：2024-09-03

申请号：US17104906

申请日：2020-11-25

申请人： Molecular Loop Biosciences, Inc.

发明人： Eric D. Boyden ,  Gregory Porreca ,  Mark Umbarger

IPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6874

CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q1/6827 ,  C12Q2537/16 ,  C12Q2545/101 ,  C12Q2565/501

摘要： The invention generally relates to methods for determining carrier status with respect to a condition or disease. In certain embodiments, the method involves exposing a sample to a plurality of molecular inversion probes capable of capturing DNA from at least one genomic region suspected of having an altered copy number and at least one internal control DNA known or suspected to have a stable copy number, capturing and sequencing DNA that binds to the molecular inversion probes, and determining a copy number state of the at least one genomic region based on the sequence results.

公开(公告)号：US12065692B2

公开(公告)日：2024-08-20

申请号：US16624682

申请日：2018-08-03

申请人： The Trustees of Columbia University in the City of New York

发明人： Wei Min ,  Fanghao Hu

IPC分类号： G01N21/65 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/686 ,  B82Y15/00 ,  B82Y30/00 ,  H04Q11/00

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/686 ,  G01N21/65 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q2565/632 ,  H04Q11/0005

摘要： A method for optical super-multiplexing using polyynes to provide enhanced images from stimulated Raman microscopy is disclosed. In some exemplary embodiments, the polyynes are organelle-targeted or spectral barcoded. Imaging can be enhanced by using the polyynes to image whole live cells or specific organelles within live cells. The polyynes can also be used in optical data storage (i.e., encoding) and identification (i.e., decoding) applications.

公开(公告)号：US20240263220A1

公开(公告)日：2024-08-08

申请号：US18431779

申请日：2024-02-02

申请人： 10x Genomics, Inc.

发明人： Mimmi OLOFSSON

IPC分类号： C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827

CPC分类号： C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The present disclosure relates in some aspects to methods for analyzing target nucleic acids in a biological sample. In some aspects, the presence, amount, and/or identity of a plurality of different target nucleic acids and/or variant sequences (e.g., SNPs or mutations) of one or more of the different target nucleic acids are analyzed in situ in a sample. Also provided are oligonucleotides, sets of oligonucleotides, compositions, and kits for use in accordance with the methods.

公开(公告)号：US12054780B2

公开(公告)日：2024-08-06

申请号：US13070240

申请日：2011-03-23

申请人： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan

发明人： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan

IPC分类号： C12Q1/6886 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/6883 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B30/00 ,  G16B30/10

CPC分类号： C12Q1/6886 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/6883 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B30/00 ,  G16B30/10 ,  C12Q2600/156 ,  C12Q1/6827 ,  C12Q2537/16

摘要： Embodiments of this invention provide methods, systems, and apparatus for determining whether a fetal chromosomal aneuploidy exists from a biological sample obtained from a pregnant female. Nucleic acid molecules of the biological sample are sequenced, such that a fraction of the genome is sequenced. Respective amounts of a clinically-relevant chromosome and of background chromosomes are determined from results of the sequencing. The determination of the relative amounts may count sequences of only certain length. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether a fetal chromosomal aneuploidy exists. Prior to sequencing, the biological sample may be enriched for DNA fragments of a particular sizes.

公开(公告)号：US20240254541A1

公开(公告)日：2024-08-01

申请号：US18588930

申请日：2024-02-27

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US12049672B2

公开(公告)日：2024-07-30

申请号：US17018692

申请日：2020-09-11

申请人： GRAIL, LLC

发明人： Rongsu Qi ,  Farooq A. Siddiqui ,  Srinka Ghosh

IPC分类号： C12Q1/6886 ,  C12Q1/6827

CPC分类号： C12Q1/6886 ,  C12Q1/6827 ,  C12Q2600/112

摘要： Methods are provided to improve the positive predictive value for cancer detection using cell-free nucleic acid samples. The methods can include the use of at least two assays. The assays can vary, for example, with respect to sensitivity, specificity, sequencing depth, analyte, and cost. An exemplary method can be used to provide an initial cancer assay with high sensitivity and a follow-up assay with high specificity in detecting cancer.

公开(公告)号：US12038438B2

公开(公告)日：2024-07-16

申请号：US17509933

申请日：2021-10-25

申请人： Bio-Rad Laboratories, Inc.

发明人： Darren R. Link ,  Michael L. Samuels

IPC分类号： G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  G01N33/542 ,  G01N33/58 ,  B01L7/00 ,  G01N21/64

CPC分类号： G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2500/00

摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20240229114A1

公开(公告)日：2024-07-11

申请号：US18617052

申请日：2024-03-26

申请人： FUJIFILM Corporation

发明人： Naoko YAMAGUCHI

IPC分类号： C12Q1/6827

CPC分类号： C12Q1/6827 ,  C12Q2600/154 ,  C12Q2600/156

摘要： An object of the present invention is to provide a production method of a biomarker set capable of diagnosing the presence or absence of cancer in a plurality of organs in a single cancer screening with higher accuracy than in the related art. The present invention provides a method for producing a biomarker set, the method including a classification step of classifying a plurality of DNA methylation degree data collected according to types of the cancer into one or more groups based on information on tissue type, an extraction step of selecting any one predetermined group from all classified groups, performing two group comparison between the predetermined group and the other group, and extracting information on a DNA position recognized to have a significant difference between both groups and a methylation state of both groups at the position, and a selection step of selecting, from the information on both groups in which the significant difference is recognized, a biomarker which satisfies a predetermined condition, as a biomarker of a cancer type to which the predetermined group belongs.