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1.发明授权公开(公告)号：US09354235B2
公开(公告)日：2016-05-31
申请号：US14209245
申请日：2014-03-13
CPC分类号： G01N33/6809 , G01N21/643 , G01N33/24 , Y10T436/143333 , Y10T436/173845 , Y10T436/176152
摘要： The invention is generally directed to a system and process for fluorometrically quantifying potentially mineralizable nitrogen for agricultural crop production. The soil analysis process measures potentially mineralizable nitrogen and calibrates the application of soil-based nitrogen for site or field specific management of nitrogen fertilizers for crops grown on a wide variety of soil textures including sandy loam, silt loam and clay soils. The spectrofluorometric system and process may be utilized for routine soil testing with a lower sample to sample variability, and the automation of the spectrofluorometric system and process allows for simultaneous determination of potentially mineralizable soil organic nitrogen, ammonium and nitrate.
摘要翻译： 本发明一般涉及一种用于氟化量化用于农作物生产的潜在可矿化氮的系统和方法。 土壤分析过程测量潜在的可矿化氮，并校准土壤氮的应用，用于在各种土壤质地（包括砂壤土，淤泥壤土和粘土）上种植的作物的氮肥的场地或田间特异性管理。 分光荧光测定系统和过程可用于常规土壤测试，其中较低的样品可以采样变异，而荧光分光光谱系统和过程的自动化允许同时测定潜在可矿化的土壤有机氮，铵和硝酸盐。
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2.发明授权Method for the determination of an analyte comprising a primary amino group, and kit for labeling said analyte 有权用于测定包含伯氨基的分析物的方法和用于标记所述分析物的试剂盒公开(公告)号：US08323979B2
公开(公告)日：2012-12-04
申请号：US12531975
申请日：2008-03-19
CPC分类号： G01N33/6809 , C07C225/16 , Y10T436/11 , Y10T436/17 , Y10T436/173845 , Y10T436/174614 , Y10T436/24 , Y10T436/25
摘要： The invention provides a method for labeling an analyte comprising a primary amino group, the method comprising: a. a labeling process comprising reacting the analyte with a dialdehyde in the presence of a label, wherein the label bears a charge, and b. an analysis process comprising subjecting the labeled analyte to MS, preferably LC-MS-MS. Herein, preferably, the labeling process comprises reacting an analyte with a dialdehyde, wherein the dialdehyde carries a label bearing a charge, to provide a labeled analyte carrying the charge.The present invention also provides a labeling method to provide a labeled analyte carrying a charge, wherein the labeling method comprises a labeling process comprising reacting an analyte with a dialdehyde, wherein the analyte comprises a primary amino group and wherein the dialdehyde carries a label bearing the charge.The dialdehyde is preferably an aromatic dialdehyde, most preferably an aromatic 1,2- or 1,3-dicarboxaldehyde. The label preferably comprises a quaternary ammonium group and/or a quaternary phosphonium group.The present invention also provides for a kit for labeling the analyte.
摘要翻译： 本发明提供了一种用于标记包含伯氨基的分析物的方法，所述方法包括：a。 一种标记方法，包括在标记存在下使分析物与二醛反应，其中标记带电荷，b。 分析方法包括将标记的分析物经受MS，优选LC-MS-MS。 在本文中，优选地，标记方法包括使分析物与二醛反应，其中二醛携带带有电荷的标记，以提供携带电荷的标记分析物。 本发明还提供了一种提供携带电荷的标记分析物的标记方法，其中标记方法包括标记方法，其包括使分析物与二醛反应，其中分析物包含伯氨基，其中二醛携带带有 收费。 二醛优选为芳族二醛，最优选芳族1,2-或1,3-二甲醛。 标签优选包含季铵基和/或季鏻基团。 本发明还提供了用于标记分析物的试剂盒。
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3.发明申请Method for the determination of an analyte comprising a primary amino group, and kit for labeling said analyte 有权用于测定包含伯氨基的分析物的方法和用于标记所述分析物的试剂盒公开(公告)号：US20100184231A1
公开(公告)日：2010-07-22
申请号：US12531975
申请日：2008-03-19
IPC分类号： G01N33/00 , C07C229/00
CPC分类号： G01N33/6809 , C07C225/16 , Y10T436/11 , Y10T436/17 , Y10T436/173845 , Y10T436/174614 , Y10T436/24 , Y10T436/25
摘要： The invention provides a method for labeling an analyte comprising a primary amino group, the method comprising: a. a labeling process comprising reacting the analyte with a dialdehyde in the presence of a label, wherein the label bears a charge, and b. an analysis process comprising subjecting the labeled analyte to MS, preferably LC-MS-MS. Herein, preferably, the labeling process comprises reacting an analyte with a dialdehyde, wherein the dialdehyde carries a label bearing a charge, to provide a labeled analyte carrying the charge.The present invention also provides a labeling method to provide a labeled analyte carrying a charge, wherein the labeling method comprises a labeling process comprising reacting an analyte with a dialdehyde, wherein the analyte comprises a primary amino group and wherein the dialdehyde carries a label bearing the charge.The dialdehyde is preferably an aromatic dialdehyde, most preferably an aromatic 1,2- or 1,3-dicarboxaldehyde. The label preferably comprises a quaternary ammonium group and/or a quaternary phosphonium group.The present invention also provides for a kit for labeling the analyte.
摘要翻译： 本发明提供了一种用于标记包含伯氨基的分析物的方法，所述方法包括：a。 一种标记方法，包括在标记存在下使分析物与二醛反应，其中标记带电荷，b。 分析方法包括将标记的分析物经受MS，优选LC-MS-MS。 在本文中，优选地，标记方法包括使分析物与二醛反应，其中二醛携带带有电荷的标记，以提供携带电荷的标记分析物。 本发明还提供了一种提供携带电荷的标记分析物的标记方法，其中标记方法包括标记方法，其包括使分析物与二醛反应，其中分析物包含伯氨基，其中二醛携带带有 收费。 二醛优选为芳族二醛，最优选芳族1,2-或1,3-二甲醛。 标签优选包含季铵基和/或季鏻基团。 本发明还提供了用于标记分析物的试剂盒。
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4.发明申请公开(公告)号：US20090258427A1
公开(公告)日：2009-10-15
申请号：US12375740
申请日：2007-07-03
申请人： Michael Schulz , Heinz-Emil Hauck
发明人： Michael Schulz , Heinz-Emil Hauck
IPC分类号： G01N33/00 , C07C49/537
CPC分类号： G01N33/6809 , G01N33/6839
摘要： The invention relates to a method and reagent mixture for the staining and thus visualisation of amino acids, peptides and similar compounds, in particular after separation by means of thin-layer chromatography. The staining is carried out using NIN-HYDRIN for the detection of amino acids, peptides or proteins in combination with at least one ionic liquid.
摘要翻译： 本发明涉及一种方法和试剂混合物，用于染色和因此显示氨基酸，肽和类似化合物，特别是通过薄层色谱分离后。 染色使用NIN-HYDRIN进行，用于与至少一种离子液体组合检测氨基酸，肽或蛋白质。
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公开(公告)号：US20060228760A1
公开(公告)日：2006-10-12
申请号：US11451975
申请日：2006-06-12
申请人： Ramin Pirzad
发明人： Ramin Pirzad
CPC分类号： G01N33/52 , G01N31/22 , G01N33/6809 , Y10S436/805 , Y10S436/808
摘要： A method of determining allergen activity in dust comprises: providing a dust sample; extracting from the dust sample at least one breakdown component or proteins or peptides; reacting the extracted at least one breakdown component with a colorimetric amine detection reagent such as TNBSA; and quantitatively measuring the intensity of any resulting coloration. The allergen activity may be gauged by the intensity of coloration.
摘要翻译： 确定粉尘中过敏原活性的方法包括：提供粉尘样品; 从灰尘样品中提取至少一种分解成分或蛋白质或肽; 将所提取的至少一种击穿组分与比色胺检测试剂如TNBSA反应; 并定量测量任何所得着色的强度。 过敏原活性可以通过着色强度来测定。
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6.发明申请Chemical reagents and methods for detection and quantification of proteins in complex mixtures 有权用于复杂混合物中蛋白质的检测和定量的化学试剂和方法公开(公告)号：US20040038319A1
公开(公告)日：2004-02-26
申请号：US10225447
申请日：2002-08-20
IPC分类号： G01N033/53 , C07K014/00
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译： 本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。 本发明还提供了使用本发明试剂的方法。 该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。 本发明另外提供了分离，鉴定和定量样品中的多肽的方法。 本发明还提供了使用本发明的试剂诊断疾病的方法。
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公开(公告)号：US06579718B1
公开(公告)日：2003-06-17
申请号：US09632927
申请日：2000-08-04
申请人： Stephen T. Yue , Thomas H. Steinberg , Wayne F. Patton , Ching-Ying Cheung , Richard P. Haugland
发明人： Stephen T. Yue , Thomas H. Steinberg , Wayne F. Patton , Ching-Ying Cheung , Richard P. Haugland
IPC分类号： G01N3348
CPC分类号： C07D401/06 , C07D209/08 , C07D209/86 , C07D213/20 , C07D215/10 , C07D277/64 , C07D417/06 , C09B23/105 , G01N33/6809 , Y10T436/25125 , Y10T436/25375
摘要： The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their strong fluorescence enhancement. Poly(amino acids) are detected in solution, in electrophoretic gels, and on solid supports, including blots and dipsticks. The present method of staining is highly sensitive, extremely facile, and relatively non-selective and can be accomplished without the use of organic solvent additives.
摘要翻译： 本发明描述了各种取代和未取代的咔唑基乙烯基染料及其用于检测和定量聚（氨基酸）的用途，包括肽，多肽和蛋白质。 标记的蛋白质或肽是高度着色的，但也通过其强荧光增强来检测。 聚（氨基酸）在溶液中，电泳凝胶和固体支持物上检测，包括印迹和浸渍。 本发明的染色方法是高度敏感的，非常容易的和相对非选择性的，并且可以在不使用有机溶剂添加剂的情况下完成。
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公开(公告)号：US5322795A
公开(公告)日：1994-06-21
申请号：US902618
申请日：1992-06-23
申请人： Donald O'Keefe
发明人： Donald O'Keefe
CPC分类号： C07K1/1077 , C07K14/501 , G01N33/6809 , G01N30/06
摘要： Proteins that cannot be resolved by conventional HPLC techniques, including reversed-phase HPLC, can be distinguished, and the quantity of a protein of interest monitored in numerous types of in-process samples, including fermentation and down-stream processing samples, by covalently binding monobromobimane to a protein of interest bearing sulfhydryl groups and chromatographing these in-process samples using reversed-phase HPLC, fluorescence detection and UV absorbance detection.
摘要翻译： 可以区分不能通过常规HPLC技术解决的蛋白质，包括反相HPLC，并且通过共价结合在许多类型的过程中样品（包括发酵和下游处理样品）中监测的感兴趣的蛋白质的量 将单溴代甲烷转化为具有巯基的感兴趣的蛋白质，并使用反相HPLC，荧光检测和UV吸光度检测对这些过程中样品进行色谱分析。
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公开(公告)号：US4713346A
公开(公告)日：1987-12-15
申请号：US825619
申请日：1986-02-03
申请人： Paul M. Gallop , Edward Henson , Rudolf Fluckiger
发明人： Paul M. Gallop , Edward Henson , Rudolf Fluckiger
CPC分类号： G01N33/92 , G01N33/52 , G01N33/6809 , Y10T436/141111 , Y10T436/142222 , Y10T436/147777 , Y10T436/173845 , Y10T436/200833 , Y10T436/201666 , Y10T436/203332 , Y10T436/24
摘要： A method of forming analyzable adducts in a mixture of compounds by contacting the mixture with a boron reagent having the formula of either ##STR1## where each X and Y is, independently, an alkyl group of 12 or fewer carbons or an aryl group of 6-20 carbons; or BZ.sub.3, where each Z is, independently, an alkyl group of 12 or fewer carbons, or an aryl group of 6-20 carbons.
摘要翻译： 通过使混合物与下式的硼试剂接触，即化合物混合物中形成可分析的加合物的方法，其中每个X和Y独立地为12个或更少个碳的烷基或6个芳基的烷基 -20碳; 或BZ3，其中每个Z独立地为12个或更少个碳的烷基或6-20个碳的芳基。
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公开(公告)号：US20170248579A1
公开(公告)日：2017-08-31
申请号：US15510490
申请日：2015-09-10
申请人： The UAB Research Foundation , THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
发明人： Peter H. King
CPC分类号： G01N33/5023 , C07K14/475 , G01N33/6809 , G01N33/6893 , G01N33/6896 , G01N2333/495 , G01N2800/28 , G01N2800/2835 , G01N2800/52
摘要： Provided herein are Amyotrophic lateral sclerosis (ALS) biomarkers and methods of using these ALS biomarkers to diagnose and treat ALS.
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