摘要:
The invention relates to a method and reagent mixture for the staining and thus visualization of amino acids, peptides and similar compounds, in particular after separation by means of thin-layer chromatography. The staining is carried out using ninhydrin for the detection of amino acids, peptides or proteins in combination with at least one ionic liquid.
摘要:
The invention relates to a method and reagent mixture for the staining and thus visualisation of amino acids, peptides and similar compounds, in particular after separation by means of thin-layer chromatography. The staining is carried out using NIN-HYDRIN for the detection of amino acids, peptides or proteins in combination with at least one ionic liquid.
摘要:
Novel separation materials for thin layer chromatography are disclosed; these consist of a support and a separation medium layer and are obtainable by the following process steps: a) cleaning of the support; b) application of a liquid film comprising a polysilicic ester to a support; c) introduction of the support with the liquid film into an atmosphere which effects hydrolysis and further polymerization of the polysilicic ester; d) hydrolysis and further polymerization of the polysilicic ester at constant temperature; e) washing of the silica layer; f) drying of the silica layer. The silica gel surface of the separation material of the invention can be modified by means of separation effectors.
摘要:
The invention concerns separating materials for thin-layer chromatography, the materials having a sorbent layer formed from sorbent particles and disposed on a carrier. A reflection intensifier, preferably magnesium tungstate and/or Bi.sub.2 O.sub.3, is added to the sorbent layer. This addition comprises at least 10 wt % relative to the amount of sorbent. In preferred embodiments, the particle size of the reflection intensifier is 3 to 5 .mu.m and the amount of the reflection intensifier is 0.6 to 15 times the amount of sorbent. These separating materials are particularly suitable for the in-situ measurement of FTIR spectra. The invention also concerns the use of these separating materials for the in-situ measurement of FTIR spectra.