公开(公告)号：US06814870B2

公开(公告)日：2004-11-09

申请号：US10658008

申请日：2003-09-09

申请人： Sheng Zhang ,  Xian Huang ,  Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser

发明人： Sheng Zhang ,  Xian Huang ,  Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser

IPC分类号： B01D1508

CPC分类号： G01N30/6095 ,  B01D15/3804 ,  B01J20/28042 ,  B01J20/285 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3244 ,  B01J20/3251 ,  B01J20/3274 ,  B01J45/00 ,  B01J2220/52 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/82 ,  G01N30/463 ,  G01N30/466 ,  G01N30/482 ,  G01N30/7266 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

摘要： A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array and a capillary tube or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modem life sciences such as proteomics and drug discover.

摘要翻译： 公开了一种基于微芯片的电喷雾离子化装置和具有亲和吸附剂的柱。 本发明包括微芯片阵列和毛细管，或单独或组合连接。 装置或柱的至少一部分具有固定的亲和吸附剂。 还提供了使用该装置的方法用于亲和捕获生物分子以满足现代生命科学如蛋白质组学和药物发现的需要。

公开(公告)号：US06773599B1

公开(公告)日：2004-08-10

申请号：US10069099

申请日：2002-05-13

申请人： Christopher Robin Lowe ,  Kim Hilary Lawden

发明人： Christopher Robin Lowe ,  Kim Hilary Lawden

IPC分类号： B01D1508

CPC分类号： G01N33/579 ,  B01J20/262 ,  B01J20/265 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  C02F1/285 ,  C07D251/50 ,  C07D251/70 ,  C07D403/12 ,  C07D403/14

摘要： An affinity ligand-matrix conjugate comprises the matrix and, conjugated thereto by the group Z, a ligand having general formula (I) wherein on X is N and the other X is N, CCL or CCn; A1 and A2 are each independently O, S or N—R1 and R1 is H, C1-6alkyl, C1-6 hydroxyalkyl, benzyl or &bgr;-phenylethyl; B1 and B2 are each independently an optionally substituted hydrocarbon lingkage containing from 1 to 10 carbon atoms; D1 is H or a primary amino, secondary amino, tertiary amino, quaternary ammonium, imidazole, guanidino or amidino group; and D2 is a secondary amino, tertiary amino, quaternary ammonium, imadazole, guanidino or amidino group; of B2-D2 is —CHCOOH—(CH2)3-4—NH2; AND p is 0 or 1. Such conjugates are useful for the separation, isolation, purification, characterisation, identification or quantification of an endotoxin.

摘要翻译： 亲和配体 - 基质缀合物包含基质，并且与基团Z共轭，具有通式（I）的配体，其中X是N，另一个X是N，CCL或CCn; A1和A2各自独立地为O，S或N-R1，R1为H，C1-6烷基，C1-6羟基烷基，苄基或β-苯乙基; B1和B2各自独立地是含有1至10个碳原子的任选取代的烃类化合物; D1是H或伯氨基，仲氨基，叔氨基，季铵，咪唑，胍基或脒基; D2是仲氨基，叔氨基，季铵，咪唑，胍基或脒基; 的B2-D2是-CHCOOH-（CH2）3-4-NH2; AND是0或1.这样的缀合物可用于内毒素的分离，分离，纯化，表征，鉴定或定量。

公开(公告)号：US06773583B2

公开(公告)日：2004-08-10

申请号：US10615820

申请日：2003-07-09

申请人： Edouard S. P. Bouvier ,  Randy E. Meirowitz ,  Patrick D. McDonald

发明人： Edouard S. P. Bouvier ,  Randy E. Meirowitz ,  Patrick D. McDonald

IPC分类号： B01D1508

CPC分类号： G01N30/482 ,  B01D15/00 ,  B01D15/08 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/62 ,  B01J2220/64

摘要： A method for removing an organic solute from a solution comprises contacting the solution with a polymer formed by copolymerizing one or more hydrophobic monomers and one or more hydrophilic monomers, whereby the solute is adsorbed onto the polymer. The solution can comprise a polar solvent such as a polar organic solvent or water or an aqueous buffer. The hydrophobic monomer can be, for example, divinylbenzene. The hydrophilic monomer can be, for example, a heterocyclic monomer, such as a vinylpyridine or N-vinylpyrrolidone.

公开(公告)号：US06758966B2

公开(公告)日：2004-07-06

申请号：US10186488

申请日：2002-07-01

申请人： Peter Myers

发明人： Peter Myers

IPC分类号： B01D1508

CPC分类号： G01N30/482 ,  B01D15/206 ,  B01D15/22 ,  B01J20/281 ,  B01J2220/54 ,  B01J2220/84 ,  G01N30/52 ,  G01N30/6004 ,  G01N30/6052 ,  G01N30/6095 ,  G01N2030/521 ,  G01N2030/524 ,  G01N2030/562 ,  G01N2030/582

摘要： A capillary column comprising monodispersed particles. The capillary is formed using a suitable substrate, wherein two plates are bonded together. At least one of the plates comprises a channel where particle beads are housed. Particle bead positions are of defined and equivalent diameter are arranged along the longitudinal axis of the housing structure. These positions are used to position packing material used to occupy the column. The particle beads themselves are of equivalent diameter.

摘要翻译： 包含单分散颗粒的毛细管柱。 使用合适的基底形成毛细管，其中两个板结合在一起。 至少一个板包括容纳颗粒珠的通道。 颗粒珠位置是限定的，等效的直径沿壳体结构的纵向轴线布置。 这些位置用于定位用于占据柱的包装材料。 颗粒珠本身具有相当的直径。

公开(公告)号：US06752922B2

公开(公告)日：2004-06-22

申请号：US10118469

申请日：2002-04-05

申请人： Jiang Huang ,  Hou-Pu Chou ,  Marc A. Unger

发明人： Jiang Huang ,  Hou-Pu Chou ,  Marc A. Unger

IPC分类号： B01D1508

CPC分类号： B01D15/08 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/387 ,  G01N2030/521 ,  Y10T137/86863 ,  Y10T436/2575

摘要： The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

摘要翻译： 本发明涉及包含微流体输送系统和与流体输送系统流体连通的色谱柱的微流体色谱装置及其制造和使用方法。 优选地，色谱柱包括OTLC，PCLC或其组合。

公开(公告)号：US06743356B1

公开(公告)日：2004-06-01

申请号：US09699212

申请日：2000-10-27

申请人： Adam M. Fermier ,  Elias H. Hochman ,  Norberto A. Guzman ,  Stephen Scypinski

发明人： Adam M. Fermier ,  Elias H. Hochman ,  Norberto A. Guzman ,  Stephen Scypinski

IPC分类号： B01D1508

CPC分类号： G01N30/466 ,  G01N30/32 ,  G01N30/34 ,  G01N30/36 ,  G01N30/74 ,  G01N2030/324 ,  G01N2030/326 ,  G01N2030/347

摘要： A high throughput high performance chromatography system operating at a high mixing pressure including a plurality of pressurized solvent reservoirs, e.g. pumps, each maintaining an associated solvent at a substantially constant pressure. A plurality of flow control valves are connected downstream or upstream of the reservoirs for controlling the flow rate of the solvent into the respective columns. The number of reservoirs equals the number of solvents. Accordingly, the system reduces the number of pumps required thereby saving money and space. Furthermore, the system increases sample throughput, accelerates method development and minimizes organic waste.

摘要翻译： 在高混合压力下操作的高通量高性能色谱系统，包括多个加压溶剂容器，例如， 泵，每个保持在基本上恒定的压力下的相关溶剂。 多个流量控制阀连接在储存器的下游或上游，用于控制溶剂进入相应柱的流速。 水库的数量等于溶剂的数量。 因此，该系统减少了所需的泵的数量，从而节省了钱和空间。 此外，该系统提高了样品通量，加速了方法开发，并最大限度地减少了有机废物。

公开(公告)号：US06723157B2

公开(公告)日：2004-04-20

申请号：US10454140

申请日：2003-06-04

申请人： Takao Tsuda ,  Motonori Munesue

发明人： Takao Tsuda ,  Motonori Munesue

IPC分类号： B01D1508

CPC分类号： B01D53/02 ,  B01D2253/10 ,  B01D2253/106 ,  B01D2253/204 ,  B01J20/0211 ,  B01J20/06 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/28023 ,  B01J20/28028 ,  B01J20/3204 ,  B01J20/3223 ,  B01J20/3225 ,  B01J20/3236 ,  B01J20/3295 ,  B01J2220/64 ,  Y10S55/05

摘要： A material is provided for capturing a chemical substance comprising fibers which carry metal alkoxide particles on the surfaces thereof. The metal alkoxide particles are made from an oligosilica, an oligozirconia, an oligotitiania, or the like. The fibers may include inorganic fibers, regenerated fibers and/or synthetic fibers. The material for capturing a chemical substance is produced by a method comprising steps of subjecting the fibers to a surface treatment for facilitating adhesion of the metal alkoxide particles to the surfaces of the fibers, forming the metal alkoxide particles on the surfaces of the fibers, and fixing the metal alkoxide particles to the surfaces of the fibers. A chemical substance-capturing tube, capable of capturing a trace of a chemical substance in a liquid or a gas, can be produced by placing the fibers, i.e., the material for capturing a chemical substance, in a hollow tube.

摘要翻译： 提供了用于捕获包含在其表面上携带金属醇盐颗粒的纤维的化学物质的材料。 金属醇盐粒子由低聚硅氧烷，低氧氧化锆，寡ia子等制成。 纤维可以包括无机纤维，再生纤维和/或合成纤维。 用于捕获化学物质的材料通过以下方法制备，所述方法包括以下步骤：使纤维进行表面处理以促进金属醇盐颗粒粘附到纤维表面，在纤维的表面上形成金属醇盐颗粒，以及 将金属醇盐颗粒固定在纤维的表面上。 可以通过将纤维（即用于捕获化学物质的材料）放置在中空管中来制造能够捕获液体或气体中的化学物质痕迹的化学物质捕获管。

公开(公告)号：US06719899B2

公开(公告)日：2004-04-13

申请号：US10339606

申请日：2003-01-10

申请人： John Davis ,  Martin John Hofmann

发明人： John Davis ,  Martin John Hofmann

IPC分类号： B01D1508

CPC分类号： B01D15/206 ,  B01D15/22 ,  F16K1/446 ,  G01N30/20 ,  G01N30/56 ,  G01N30/6004 ,  G01N30/6017 ,  G01N2030/027 ,  G01N2030/565

摘要： An access valve suitable for controlling fluid flow into and out of a chromatography column has a central axially movable probe with a head acting as a spool valve in a barrel. Axial movement of the probe adjusts the valve between a fully open condition, in which both a first conduit extending through the probe and a second conduit defined around the probe are open to the column interior, a partly open condition in which a second sealing land on the probe closes the second conduit, and a fully closed position in which both conduits are closed. The three positions are useful for packing and unpacking chromatography media into and from the column. In the closed condition of the valve, the first and second conduits communicate with one another so that the valve interior can be cleaned while the column is operating.

摘要翻译： 适于控制进入和离开色谱柱的流体流动的进入阀具有中心可轴向移动的探针，其头部用作筒中的滑阀。 探针的轴向运动在完全打开状态之间调整阀，其中延伸穿过探针的第一导管和围绕探针定义的第二导管与柱内部打开，部分打开状态，其中第二密封平台 探头关闭第二导管，以及完全关闭位置，其中两个导管都封闭。 这三个位置可用于将色谱介质打包和拆开进入和离开色谱柱。 在阀的关闭状态下，第一和第二管道彼此连通，使得阀内部可以在柱运行时被清洁。

公开(公告)号：US06709584B2

公开(公告)日：2004-03-23

申请号：US10182551

申请日：2002-07-30

申请人： Rolf Güller ,  Séraphin Munch ,  Philippe Jablonski ,  Josef Schröer

发明人： Rolf Güller ,  Séraphin Munch ,  Philippe Jablonski ,  Josef Schröer

IPC分类号： B01D1508

CPC分类号： G01N30/94 ,  G01N30/91

摘要： A device for analyzing substances by thin layer chromatography comprises a solvent trough with two solvent channels, wherein two wall plates are mounted in the solvent trough, enabling thin layer plates to be arranged obliquely with respect to the horizontal in the solvent channels. The substance to be analyzed can thus be dropped or applied onto the thin layer plates from above, and solvent can be supplied to the solvent channels from above at a solvent supply point. The upward migration of components of the substance to be analyzed can thus be started without changing the position of the thin layer plate for this purpose.

摘要翻译： 通过薄层色谱分析物质的装置包括具有两个溶剂通道的溶剂槽，其中两个壁板安装在溶剂槽中，使薄层板相对于溶剂通道中的水平面倾斜布置。 因此，待分析的物质可以从上方滴落或施加到薄层板上，并且可以在溶剂供应点从上方向溶剂通道提供溶剂。 因此可以开始待分析物质的组分的向上迁移，而不改变薄层板的位置用于此目的。

公开(公告)号：US06706191B1

公开(公告)日：2004-03-16

申请号：US09830863

申请日：2001-08-23

申请人： Patrik Leijon

发明人： Patrik Leijon

IPC分类号： B01D1508

CPC分类号： B01D15/1807 ,  B01D15/203 ,  B01D2215/021 ,  G01N30/58 ,  G01N30/6017 ,  G01N2030/565 ,  G01N2030/328

摘要： A liquid chromatographic process performed at least partly in a fluidised bed contained in a vessel and comprising particle fluidised by an upwardly directed liquid flow, said process comprising (a) a capture step in which one or more compounds of a sample are captured by the particles and (b) a wash and/or releasing step in which the particles is in form of a fluidised bed through which a liquid flow is passing. The characteristic feature is that the liquid (liquid 1) used in the wash or the releasing step (step 1) is immediately followed by a liquid (liquid 2, step 2) having a higher density than liquid 1 while maintaining the bed in a fluidised state. A liquid chromatographic process having an actual sequence of steps comprising (c) at least a capture step in which a sample deriving from an animal is bound to the particles and (d) two consecutive steps (step 1 and step 2) in which the bed is fluidised by a liquid flow passing through said vessel. The characteristic feature of the process is that the liquid used in step 2 (liquid 2) has a density that is higher than the density of the liquid used in step 1 (liquid 1).

摘要翻译： 至少部分地在包含在容器中的流化床中进行的液相色谱法，其包含通过向上指向的液体流动流化的颗粒，所述方法包括（a）捕获步骤，其中样品的一种或多种化合物被颗粒捕获 和（b）洗涤和/或释放步骤，其中所述颗粒是液体流通过的流化床的形式。 其特征在于，在洗涤或脱模步骤（步骤1）中使用的液体（液体1）紧接着是具有比液体1更高的密度的液体（液体2，步骤2），同时将床保持在流化 州。 一种液相色谱方法，其具有实际的步骤顺序步骤，包括（c）至少一个捕获步骤，其中将衍生自动物的样品与颗粒结合，和（d）两个连续步骤（步骤1和步骤2），其中床 被流过所述容器的液体流量化。 该方法的特征在于步骤2（液体2）中使用的液体的密度高于步骤1（液体1）中使用的液体的密度。