发明授权
US08323979B2 Method for the determination of an analyte comprising a primary amino group, and kit for labeling said analyte
有权
用于测定包含伯氨基的分析物的方法和用于标记所述分析物的试剂盒
- 专利标题: Method for the determination of an analyte comprising a primary amino group, and kit for labeling said analyte
- 专利标题(中): 用于测定包含伯氨基的分析物的方法和用于标记所述分析物的试剂盒
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申请号: US12531975申请日: 2008-03-19
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公开(公告)号: US08323979B2公开(公告)日: 2012-12-04
- 发明人: Thomas Ivo Franciscus Hubert Cremers , Lutske Aafke Anna de Jong , Wijnand Sjoerd Faber
- 申请人: Thomas Ivo Franciscus Hubert Cremers , Lutske Aafke Anna de Jong , Wijnand Sjoerd Faber
- 申请人地址: NL AV Groningen
- 专利权人: Brains Online Holding B.V.
- 当前专利权人: Brains Online Holding B.V.
- 当前专利权人地址: NL AV Groningen
- 代理机构: Nixon Peabody LLP
- 代理商 Khaled Shami
- 优先权: EP07104418 20070319; EP07123996 20071221
- 国际申请: PCT/NL2008/050155 WO 20080319
- 国际公布: WO2008/094043 WO 20080807
- 主分类号: G01N33/00
- IPC分类号: G01N33/00 ; G01N30/72
摘要:
The invention provides a method for labeling an analyte comprising a primary amino group, the method comprising: a. a labeling process comprising reacting the analyte with a dialdehyde in the presence of a label, wherein the label bears a charge, and b. an analysis process comprising subjecting the labeled analyte to MS, preferably LC-MS-MS. Herein, preferably, the labeling process comprises reacting an analyte with a dialdehyde, wherein the dialdehyde carries a label bearing a charge, to provide a labeled analyte carrying the charge.The present invention also provides a labeling method to provide a labeled analyte carrying a charge, wherein the labeling method comprises a labeling process comprising reacting an analyte with a dialdehyde, wherein the analyte comprises a primary amino group and wherein the dialdehyde carries a label bearing the charge.The dialdehyde is preferably an aromatic dialdehyde, most preferably an aromatic 1,2- or 1,3-dicarboxaldehyde. The label preferably comprises a quaternary ammonium group and/or a quaternary phosphonium group.The present invention also provides for a kit for labeling the analyte.
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