公开(公告)号：US12024498B2

公开(公告)日：2024-07-02

申请号：US17986325

申请日：2022-11-14

Applicant: OHIO STATE INNOVATION FOUNDATION

Inventor： Daniel W. Conroy ,  Christopher P. Jaroniec

IPC: C07D401/12 ,  C07D211/94 ,  C07D221/20 ,  C07D491/20 ,  G01N24/12 ,  G01R33/62

CPC classification number: C07D401/12 ,  C07D211/94 ,  C07D221/20 ,  C07D491/20 ,  G01N24/12 ,  G01R33/62

Abstract: Disclosed are methods for performing dynamic nuclear polarization using the polarizing agents described herein. In general, the methods involve (a) providing a frozen sample in a magnetic field, wherein the frozen sample includes a polarizing agent described herein and an analyte with at least one spin half nucleus; (b) polarizing the at least one spin half nucleus of the analyte by irradiating the frozen sample with radiation having a frequency that excites electron spin transitions in the polarizing agent; (c) optionally melting the sample to produce a molten sample; and (d) detecting nuclear spin transitions in the at least one spin half nucleus of the analyte in the frozen or molten sample. In certain embodiments, the polarizing agents can be peptide-based. In these embodiments, the polarizing agents can be readily prepared by solid-phase peptide synthesis.

公开(公告)号：US10036787B2

公开(公告)日：2018-07-31

申请号：US14905968

申请日：2014-07-09

Applicant: BRUKER BIOSPIN GMBH

Inventor： Alexander Krahn ,  Frank Engelke

IPC: G01R33/34 ,  G01R33/28 ,  G01R33/345 ,  G01R33/62 ,  G01N24/12

CPC classification number: G01R33/34 ,  G01N24/12 ,  G01R33/282 ,  G01R33/345 ,  G01R33/62

Abstract: An NMR (nuclear magnetic resonance) probe head has a microwave resonator with at least two elements which are reflective in the microwave range, at least one of which is focusing. The reflective elements at least partly delimit a resonance volume of the microwave resonator. At least one of the reflective elements is a DBR (“Distributed Bragg Reflector”), and the NMR probe head has at least one NMR coil integrated into the DBR. The NMR detection coil can thereby be positioned particularly near to the sample and the distortions of the static field by resonator components are reduced, such that the detection sensitivity and the spectral resolution of the experiment are significantly improved.

公开(公告)号：US09554726B2

公开(公告)日：2017-01-31

申请号：US13721173

申请日：2012-12-20

Applicant: Johann Wolfgang Goethe-Universität Frankfurt am Main

Inventor： Jan Krummenacker ,  Thomas Prisner ,  Vasyl Denysenkov ,  Laura Schreiber ,  Kerstin Münnemann

IPC: A61B5/05 ,  A61B5/055 ,  G01R33/28 ,  G01R33/62 ,  G01N24/12 ,  G01R33/56

CPC classification number: A61B5/055 ,  G01N24/12 ,  G01R33/282 ,  G01R33/5601 ,  G01R33/62

Abstract: A hyperpolarized liquid contrast agent is for use in a MRT device. The liquid contrast agent passes through a conduit of a MW resonator in the magnetic field of the MRT device. A microwave with a frequency of at least 40 GHz couples into the MW resonator for polarizing the liquid contrast agent upon passage through the conduit in the MW resonator using DNP. The contrast agent is polarized in a continuous passage in the MW resonator and administered immediately. A MW mode is formed in the MW resonator which has an antinode in the magnetic field strength and a node in the electric field strength. The power of the introduced microwave and coupling of the microwave into the resonator are adjusted such that in the area of the line, an amplitude of the MW magnetic field strength B 1 ≥ 1.5 · 10 - 2 ⁢ Ts ⁢ 1 T 1 , e results, wherein T1,e is the relaxation time of the DNP-active electrons.

Abstract translation: 超极化液体造影剂用于MRT装置。 液体造影剂通过MRT装置的磁场中的MW谐振器的导管。 具有至少40GHz的频率的微波耦合到MW谐振器中，用于在通过使用DNP的MW谐振器中的导管通过时使液体造影剂偏振。 造影剂在MW谐振器的连续通道中极化，并立即施用。 在MW谐振器中形成MW模式，其具有磁场强度中的波腹和电场强度的节点。 引入微波的功率和微波耦合到谐振器中的功率被调整为使得在该区域中，MW磁场强度B1> = 1.5·10-2·Ts 1 / T1的振幅为e，其中e T1，e是DNP活性电子的弛豫时间。

公开(公告)号：US20130184565A1

公开(公告)日：2013-07-18

申请号：US13721173

申请日：2012-12-20

Applicant: Johann Wolfgang Goethe-Universitat Frankfurt am Main

Inventor： Jan Krummenacker ,  Thomas Prisner ,  Vasyl Denysenkov ,  Laura Schreiber ,  Kerstin Münnemann

IPC: A61B5/055

CPC classification number: A61B5/055 ,  G01N24/12 ,  G01R33/282 ,  G01R33/5601 ,  G01R33/62

Abstract: A hyperpolarized liquid contrast agent is for use in a MRT device. The liquid contrast agent passes through a conduit of a MW resonator in the magnetic field of the MRT device. A microwave with a frequency of at least 40 GHz couples into the MW resonator for polarizing the liquid contrast agent upon passage through the conduit in the MW resonator using DNP. The contrast agent is polarized in a continuous passage in the MW resonator and administered immediately. A MW mode is formed in the MW resonator which has an antinode in the magnetic field strength and a node in the electric field strength. The power of the introduced microwave and coupling of the microwave into the resonator are adjusted such that in the area of the line, an amplitude of the MW magnetic field strength B1≧1.5·10−2 Ts 1/T1,e results, wherein T1,e is the relaxation time of the DNP-active electrons.

Abstract translation: 超极化液体造影剂用于MRT装置。 液体造影剂通过MRT装置的磁场中的MW谐振器的导管。 具有至少40GHz的频率的微波耦合到MW谐振器中，用于在通过使用DNP的MW谐振器中的导管通过时使液体造影剂偏振。 造影剂在MW谐振器的连续通道中极化，并立即施用。 在MW谐振器中形成MW模式，其具有磁场强度中的波腹和电场强度的节点。 引入微波的功率和微波耦合到谐振器中的功率被调整为使得在该区域中，MW磁场强度B1> = 1.5·10-2·Ts 1 / T1的振幅为e，其中e T1，e是DNP活性电子的弛豫时间。

公开(公告)号：US20120068706A1

公开(公告)日：2012-03-22

申请号：US13262411

申请日：2010-03-16

Applicant: Thomas Prisner ,  Vasyl Denysenkov

Inventor： Thomas Prisner ,  Vasyl Denysenkov

IPC: G01R33/46 ,  G01R33/34

CPC classification number: G01N24/12 ,  G01R33/282 ,  G01R33/345 ,  G01R33/62

Abstract: A double-resonance structure 10 for DNP-NMR and/or ENDOR experiments is described. The double-resonance structure 10 comprises a microwave resonator 30 for generating electromagnetic fields suitable for EPR, and an HF resonator 12 for generating electromagnetic fields suitable for NMR. The HF resonator 12 comprises a plurality of electrically conductive strips 14, which are electrically connected so that an HF current can be generated in the plurality of strips 14 such that the HF currents flow in the same direction in the individual strips 14 at the same time. A section of the HF resonator 12 at the same time forms a part of the microwave resonator 30.

Abstract translation: 描述了用于DNP-NMR和/或ENDOR实验的双共振结构10。 双谐振结构10包括用于产生适合于EPR的电磁场的微波谐振器30和用于产生适用于NMR的电磁场的HF谐振器12。 HF谐振器12包括多个导电条14，其电连接，使得可以在多个条14中产生HF电流，使得HF电流同时在各个条14中沿相同的方向流动 。 HF谐振器12的一部分同时形成微波谐振器30的一部分。

公开(公告)号：US20050232864A1

公开(公告)日：2005-10-20

申请号：US10512009

申请日：2003-04-15

Applicant: Bill Clark ,  Klaes Golman ,  Mathilde Lerche ,  Mike Looker ,  Mike O'Sullivan ,  Albie Santos ,  Rolf Servin ,  Mikkel Thaning ,  Jan-Henrik Ardenkjaer-Larsen

Inventor： Bill Clark ,  Klaes Golman ,  Mathilde Lerche ,  Mike Looker ,  Mike O'Sullivan ,  Albie Santos ,  Rolf Servin ,  Mikkel Thaning ,  Jan-Henrik Ardenkjaer-Larsen

IPC: G01R33/28 ,  A61K49/00 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/48 ,  C12Q1/68 ,  G01N20060101 ,  G01N24/08 ,  G01R33/465

CPC classification number: G01N24/08 ,  G01N24/12 ,  G01R33/281 ,  G01R33/465 ,  G01R33/62

Abstract: The invention relates to methods for phenotyping by determining protein activity in vivo using at least one probe compound and enhancing the nuclear polarisation of NMR active nuclei present in the probe compound (hereinafter termed “hyperpolarisation”) prior to NMR analysis.

Abstract translation: 本发明涉及通过使用至少一种探针化合物测定体内蛋白质活性并增强核磁共振分析前存在于探针化合物中的NMR活性核（以下称为“超极化”）的核偏振的表型分析方法。

公开(公告)号：US20180149717A1

公开(公告)日：2018-05-31

申请号：US15576018

申请日：2015-05-22

Applicant: Universität Ulm

Inventor： Fedor JELEZKO ,  Martin PLENIO ,  Ilai SCHWARTZ ,  Qiong CHEN ,  Alex RETZKER

IPC: G01R33/28 ,  G01R33/62 ,  G01R33/56 ,  G01R33/26

CPC classification number: G01R33/282 ,  G01N24/12 ,  G01R33/26 ,  G01R33/5601 ,  G01R33/62

Abstract: A method of hyperpolarisation of nuclear spins in one or more particle(s) moving relatively to a polarisation structure, wherein a polarisation of electron spins in the polarisation structure is transferred to the nuclear spins in the particle(s), wherein for one or more of the moving particle(s) within 20 nm from a surface of the polarisation structure, the correlation time of the interaction with the nearest polarisation structure electron spin due to the molecular motion is larger than the inverse of the nuclear Larmor frequency; the electron spins in the polarisation structure are polarised above thermal equilibrium; and the polarisation transfer is performed resonantly.

公开(公告)号：US09778335B2

公开(公告)日：2017-10-03

申请号：US14400471

申请日：2013-05-09

Applicant: The Trustees of The University of Pennsylvania

Inventor： Andrew Joshua Wand

IPC: G01R33/465 ,  G01R33/28 ,  G01N24/12 ,  G01R33/44 ,  G01R33/62

CPC classification number: G01R33/465 ,  G01N24/12 ,  G01R33/282 ,  G01R33/445 ,  G01R33/62

Abstract: The polarization of nuclear spins of a material may be enhanced by encapsulating the material within a reverse micelle.

公开(公告)号：US09296688B2

公开(公告)日：2016-03-29

申请号：US14403686

申请日：2013-05-28

Applicant: Adam B. Braunschweig ,  Stephen Rieth ,  Matthew Miner

Inventor： Adam B. Braunschweig ,  Stephen Rieth ,  Matthew Miner

IPC: C07D207/335 ,  C07D403/14 ,  G01N24/12 ,  A61K31/4025 ,  G01N33/53

CPC classification number: C07D207/335 ,  A61K31/4025 ,  C07D403/14 ,  G01N24/12 ,  G01N33/5308 ,  G01N2400/00

Abstract: The present invention is directed to a new class of synthetic carbohydrate receptor compounds comprising Formula I as described herein: (I). Other aspects of the present invention relate to pharmaceutical compositions and pharmaceutical delivery vehicles comprising the compound of Formula (I) The present invention is also directed to methods of treatment and diagnosis that involve the administration of a compound of Formula (I).

Abstract translation: 本发明涉及一类新的合成的碳水化合物受体化合物，其包含本文所述的式I：（I）。 本发明的其它方面涉及包含式（I）化合物的药物组合物和药物递送载体。本发明还涉及涉及给予式（I）化合物的治疗和诊断方法。

公开(公告)号：US20160054402A1

公开(公告)日：2016-02-25

申请号：US14781127

申请日：2014-04-07

Applicant: RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK

Inventor： Carlos A. Meriles

IPC: G01R33/30 ,  G01N24/08

CPC classification number: G01R33/302 ,  G01N24/08 ,  G01N24/12 ,  G01R33/282 ,  G01R33/307 ,  G01R33/62

Abstract: A method and apparatus for polarizing nuclear or electronic spins is disclosed. An analyte is passed near a surface that has a plurality of spin defect centers implanted within 10 nm of the surface. The spin defect centers are exposed to a magnetic field and illumination to produce polarized spins. The polarized spins then induce spin polarization in the analyte.

Abstract translation: 公开了一种用于偏振核或电子自旋的方法和装置。 分析物在表面附近通过，该表面具有植入表面10nm内的多个自旋缺陷中心。 旋转缺陷中心暴露于磁场和照明以产生极化自旋。 然后，极化旋转在分析物中诱导自旋极化。