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    Separation Process
    2.
    发明申请
    Separation Process 有权
    分离过程

    公开(公告)号：US20140342413A1

    公开(公告)日：2014-11-20

    申请号：US14232426

    申请日：2012-07-16

    申请人： Plaxica Limited

    发明人： Edward Leslie Marshall ,  Jade Jocelyn Afriye Osei-Tutu ,  Stephen Alexander Calder Smith

    IPC分类号： C12P7/62

    CPC分类号： C12P7/62 ,  C12P7/56 ,  C12P17/06 ,  C12P41/003

    摘要： A process for treating a mixture of R,R- and S,S-lactide is provided. The process involves contacting the lactide mixture with an aliphatic alcohol and/or an alkoxide to produce a mixture of R,R- and S,S-aliphatic ester of lactyllactic acid, subsequently contacting the mixture of R,R- and S,S-aliphatic ester of lactyllactic acid with an enzyme to produce a mixture comprising aliphatic ester of lactic acid corresponding to one lactide enantiomer and the aliphatic ester of lactyllactic acid corresponding to the other lactide enantiomer, and recovering the product. Also provided are processes for the production of S-lactic acid, S,S-lactide, poly-S-lactic acid, R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex polylactic acid.

    摘要翻译： 提供了一种用于处理R，R-和S，S-丙交酯的混合物的方法。 该方法包括使丙交酯混合物与脂族醇和/或醇盐接触以产生乳糖基乳酸的R，R-和S，S-脂族酯的混合物，随后将R，R-和S的混合物S- 将乳酸乳酸的脂肪族酯与酶反应，生成包含对应于一种丙交酯对映异构体的乳酸脂肪酸酯和对应于其它丙交酯对映异构体的乳糖乳酸脂肪酸酯的混合物，并回收该产物。 还提供了用于生产S-乳酸，S，S-丙交酯，聚S-乳酸，R-乳酸，R，R-丙交酯，聚R-乳酸和立体络合物聚乳酸的方法。

    Method for preparing optically active compound
    3.
    发明授权
    Method for preparing optically active compound 失效
    光学活性化合物的制备方法

    公开(公告)号：US07846701B2

    公开(公告)日：2010-12-07

    申请号：US11667688

    申请日：2005-10-31

    申请人： Shigeru Tanaka ,  Shinya Watanabe ,  Ken-ichi Yamamoto ,  Hiroyuki Matsuda

    发明人： Shigeru Tanaka ,  Shinya Watanabe ,  Ken-ichi Yamamoto ,  Hiroyuki Matsuda

    IPC分类号： C12P7/26

    CPC分类号： C12P7/26 ,  C12P41/003

    摘要： According to the present invention, there is provided a method for preparing an optically active compound, characterized in that said method comprises permitting a mixture of optical isomers relative to the carbon atom in the β-position in relation to the carbon atom bound to an esterified hydroxy group of an enol ester to hydrolyse either one optical isomer preferentially in the presence of an enzyme and allowing the carbonyl compound resulting from such hydrolysis to enrich the proportion of its isomer having either one configuration in the β-position in relation to the carbonyl group or allowing the enol ester left non-hydrolyzed to enrich the proportion of its isomer having either one configuration on the carbon atom in the β-position in relation to the carbon atom to which the esterified hydroxyl group bonds.

    摘要翻译： 根据本发明，提供了一种制备光学活性化合物的方法，其特征在于所述方法包括允许相对于与一个碳原子结合的碳原子相对于碳原子的光学异构体的混合物 烯醇酯的酯化羟基在酶的存在下优先水解任一种旋光异构体，并使得由这种水解产生的羰基化合物能够富集其相对于化合物中的任何一种构型的其异构体的比例 使烯醇酯残留未水解以富集其相对于与酯化羟基键合的碳原子相关的碳原子上具有一个构型的异构体的比例。

    Process for producing lactonase and utilization thereof
    4.
    发明申请
    Process for producing lactonase and utilization thereof 审中-公开
    生产内酯酶的方法及其利用

    公开(公告)号：US20070134775A1

    公开(公告)日：2007-06-14

    申请号：US10547424

    申请日：2004-03-03

    申请人： Sakayu Shimizu ,  Michihiko Kataoka ,  Keiji Sakamoto

    发明人： Sakayu Shimizu ,  Michihiko Kataoka ,  Keiji Sakamoto

    IPC分类号： C12P17/06 ,  C12N9/16 ,  C12N1/18

    CPC分类号： C12P41/003 ,  C12N9/18 ,  C12P17/04

    摘要： It has been required to economically produce a large amount of optically active γ-lactone derivatives (for example, pantolactone), which are useful as intermediates in synthesizing useful substances such as pharmaceutical drugs. To achieve this object, it is advantageous to employ an enzymatic technique of asymmetric hydrolysis with a hydrolyzing enzyme lactonase. However, it remains troublesome to prepare the enzyme or utilize a microorganism capable of producing the enzyme. Also, it is difficult to obtain a sufficient and stable enzymatic activity in the case of using recombination techniques. In producing lactonase, which asymmetrically hydrolyzes a γ-lactone derivative such as racemic pantolactone selectively, by a recombination technique, both mature lactonase DNA and signal peptide region DNA are transferred into a host. Thus, a stable lactonase activity comparable to naturally-occurring one can be achieved and a transformant sustaining a high enzyme activity in a stable manner can be acquired, thereby allowing the efficient and industrially advantageous asymmetric synthesis of γ-lactone derivatives.

    摘要翻译： 需要经济地生产大量的用作合成有用物质如药物的中间体的光学活性γ-内酯衍生物（例如泛酸内酯）。 为了达到这个目的，使用水解酶内酯酶的不对称水解酶技术是有利的。 然而，制备酶或利用能够产生酶的微生物仍然是麻烦的。 此外，在使用重组技术的情况下，难以获得足够稳定的酶活性。 在制备内含子选择性地水解γ-内酯衍生物如外消旋泛酸内酯的内切酶中，通过重组技术，将成熟内含酶DNA和信号肽区DNA两者转移到宿主中。 因此，可以获得与天然存在的活性相当的稳定的内含酶活性，并且可以获得以稳定的方式维持高酶活性的转化体，从而允许有效和工业上有利的γ-内酯衍生物的不对称合成。

    Monopartite RNA virus transformation vectors
    8.
    发明申请
    Monopartite RNA virus transformation vectors 审中-公开
    单分子RNA病毒转化载体

    公开(公告)号：US20030150019A1

    公开(公告)日：2003-08-07

    申请号：US10280679

    申请日：2002-10-24

    申请人： LARGE SCALE BIOLOGY CORPORATION

    发明人： Thomas H. Turpen ,  Ann Myers Turpen ,  Stephen J. Garger ,  Laurence K. Grill ,  Jonathan Donson ,  William O. Dawson ,  George L. Granthan

    IPC分类号： A01H001/00 ,  C12N015/82 ,  C12N007/00

    CPC分类号： C12Y114/18001 ,  C07K14/005 ,  C07K14/415 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/1074 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12N15/8203 ,  C12N15/86 ,  C12N2770/00022 ,  C12N2770/36122 ,  C12N2830/00 ,  C12N2830/60 ,  C12P41/003 ,  C12Y301/11002 ,  C12Y302/01031 ,  C12Y304/21069

    摘要： The present invention relates to a recombinant viral nucleic acid selected from a (null) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.

    摘要翻译： 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此不具有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。 本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被本发明的病毒感染的宿主，通过表达病毒核酸产生的基因产物和 通过生长受感染的宿主来生产所需产品的方法。

    Production of lysosomal enzymes in plants by transient expression
    9.
    发明申请
    Production of lysosomal enzymes in plants by transient expression 失效
    通过瞬时表达在植物中产生溶酶体酶

    公开(公告)号：US20020088024A1

    公开(公告)日：2002-07-04

    申请号：US09993059

    申请日：2001-11-13

    发明人： Stephen J. Garger ,  Thomas H. Turpen ,  Monto H. Kumagai

    IPC分类号： A01H005/00 ,  C07H021/04 ,  C12N009/24 ,  C12N007/00 ,  C12N015/82

    CPC分类号： C12Y302/01045 ,  C07K14/005 ,  C07K14/415 ,  C07K14/445 ,  C07K2319/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/1074 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2465 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8289 ,  C12N15/86 ,  C12N2770/00022 ,  C12N2770/32722 ,  C12P41/003 ,  C12Y114/18001 ,  C12Y302/01022 ,  C12Y302/01031 ,  C12Y304/21069

    摘要： The invention relates to -galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human null galactosidase nucleotide sequences. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.

    摘要翻译： 本发明涉及在羧基末端截短的半乳糖苷酶和生产酶促活化的重组人和动物溶酶体酶，其涉及重组表达构建体的构建和表达，其包含编码植物表达系统中人或动物溶酶体酶的序列。 植物表达系统提供翻译后修饰和加工以产生显示酶活性的重组基因产物。 本发明通过其中转基因烟草植物表达包含人葡糖脑苷脂酶核苷酸序列的重组表达构建体的实施例来证明。 本发明还通过其中转染的烟草植物表达包含人α半乳糖苷酶核苷酸序列的重组病毒表达构建体的实施例来证明。 根据本发明生产的重组溶酶体酶可用于多种目的，包括但不限于用于治疗性治疗人和动物溶酶体贮积病的酶替代疗法。

    Method and preparing chiral esters
    10.
    发明申请
    Method and preparing chiral esters 失效
    方法和制备手性酯

    公开(公告)号：US20010012898A1

    公开(公告)日：2001-08-09

    申请号：US09726412

    申请日：2000-12-01

    发明人： Jai Wook Park ,  Mahn-Joo Kim ,  Jeong Hwan Koh ,  Hyun Min Jung

    IPC分类号： C07D207/12 ,  C07D207/24 ,  C07D207/36

    CPC分类号： C12P41/003 ,  C12P7/62

    摘要： The present invention relates to a method for preparing a chiral ester and more particularly, the method for preparing an optically pure chiral ester from an alkenyl ester at a high yield by mixing and reacting: an alkenyl ester; a ruthenium complex to activate reduction reaction of said alkenyl ester and racemization; a lipase to acylate selectively one of enantiomers of said alkenyl ester; and a reducing agent to supply a hydride to said ruthenium complex. Said optically pure chiral ester of the present invention can be prepared by one-step synthesis from various types of alkenyl esters at a high yield.

    摘要翻译： 本发明涉及一种制备手性酯的方法，更具体地说，涉及通过混合和反应从链烯基酯以高产率制备光学纯手性酯的方法：烯基酯; 用于活化所述烯基酯的还原反应的钌络合物和外消旋化; 选择性地将所述烯基酯的对映体之一酰化的脂肪酶; 以及向所述钌络合物供给氢化物的还原剂。 本发明的所述光学纯手性酯可以通过以高收率从各种类型的烯基酯进行一步合成来制备。