公开(公告)号：US20140140961A1

公开(公告)日：2014-05-22

申请号：US14057851

申请日：2013-10-18

申请人： THE TEXAS A&M UNIVERSITY SYSTEM

发明人： CARLOS F. GONZALEZ ,  STEPHEN J. AHERN ,  MAYUKH DAS ,  RYLAND F. YOUNG, III ,  TUSHAR SURVA BHOWMICK

IPC分类号： C12N7/00 ,  C12R1/91

CPC分类号： A01N63/00 ,  C12N7/00 ,  C12N2795/10221 ,  C12N2795/10231 ,  C12N2795/10233 ,  C12N2795/10251 ,  C12N2795/10321 ,  C12N2795/10331 ,  C12R1/91

摘要： The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.

摘要翻译： 本发明提供了用于开发用于控制由Xylella fastidiosa引起的植物病害的基于有毒性噬菌体的处理的方法。 本发明进一步提供了在黄单胞菌属细菌宿主中分离和繁殖毒蕈毒素至十字戟科的方法，并用于治疗或减少植物中快速疟原虫感染的症状。 本发明进一步提供了分离和繁殖毒性至黄单胞菌属（Xanthomonas axonopodis pv）的方法。 柠檬酸和用于治疗或减轻黄单胞菌属的症状。 柑橘感染在植物中。

公开(公告)号：US20160302426A1

公开(公告)日：2016-10-20

申请号：US15174564

申请日：2016-06-06

申请人： The Texas A&M University System

发明人： Carlos F. Gonzalez ,  Stephen J. Ahern ,  Mayukh Das ,  Ryland F. Young ,  Tushar Surva Bhowmick

IPC分类号： A01N63/00 ,  C12N7/00 ,  C12R1/91

CPC分类号： A01N63/00 ,  C12N7/00 ,  C12N2795/10221 ,  C12N2795/10231 ,  C12N2795/10233 ,  C12N2795/10251 ,  C12N2795/10321 ,  C12N2795/10331 ,  C12R1/91

摘要： The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.

摘要翻译： 本发明提供了用于开发用于控制由Xylella fastidiosa引起的植物病害的基于有毒性噬菌体的处理的方法。 本发明进一步提供了在黄单胞菌属细菌宿主中分离和繁殖毒蕈毒素至十字戟科的方法，并用于治疗或减少植物中快速疟原虫感染的症状。 本发明进一步提供了分离和繁殖毒性至黄单胞菌属（Xanthomonas axonopodis pv）的方法。 柠檬酸和用于治疗或减轻黄单胞菌属的症状。 柑橘感染在植物中。

公开(公告)号：US20160122729A1

公开(公告)日：2016-05-05

申请号：US14990274

申请日：2016-01-07

申请人： Sample6 Technologies, Inc.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making aa plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。

公开(公告)号：US20170298456A1

公开(公告)日：2017-10-19

申请号：US15470750

申请日：2017-03-27

申请人： The Charles Stark Draper Laboratory, Inc.

发明人： Jason W. Holder ,  Jason O. Fiering

IPC分类号： C12Q1/70 ,  B01L3/00

CPC分类号： C12Q1/70 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/12 ,  C12N7/00 ,  C12N15/11 ,  C12N15/66 ,  C12N15/86 ,  C12N15/902 ,  C12N2310/20 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/80 ,  C12Y301/00

摘要： The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.

公开(公告)号：US20170283779A1

公开(公告)日：2017-10-05

申请号：US15470685

申请日：2017-03-27

申请人： The Charles Stark Draper Laboratory, Inc.

发明人： JASON HOLDER ,  Connor McBrine ,  Sarah Gruszka ,  Miles Rogers ,  Nicole Billings

IPC分类号： C12N7/00 ,  C12N15/90 ,  C12N15/66 ,  C12N15/11

CPC分类号： C12Q1/70 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/12 ,  C12N7/00 ,  C12N15/11 ,  C12N15/66 ,  C12N15/86 ,  C12N15/902 ,  C12N2310/20 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/80 ,  C12Y301/00

摘要： The present disclosure provides methods and kits for generating recombinant bacteriophage genomes.

公开(公告)号：US20240279620A1

公开(公告)日：2024-08-22

申请号：US18651814

申请日：2024-05-01

申请人： Adaptive Phage Therapeutics, Inc.

发明人： Joseph Robert Fackler ,  Carl Merril ,  Jarrar Haider ,  Viet Dang

IPC分类号： C12N7/00 ,  A61K35/76 ,  A61P31/04 ,  B01D61/20 ,  B01D69/08

CPC分类号： C12N7/00 ,  A61K35/76 ,  A61P31/04 ,  B01D61/20 ,  B01D69/08 ,  B01D2311/2676 ,  B01D2311/2688 ,  B01D2315/10 ,  C12N2795/10132 ,  C12N2795/10151 ,  C12N2795/10232 ,  C12N2795/10251 ,  C12N2795/10332 ,  C12N2795/10351

摘要： A method of recovering viable phage from, for example, a crude phage preparation such as a lysate resulting from amplification of phage in bacterial cell culture is disclosed. The method may be “universal”; that is, applicable to the purification of a broad range of phage species and strains. The phage product resulting from the method may have an acceptably low endotoxin titer (e.g. less than 500 EU/ml) and sufficiently high phage titer (e.g. >1×109 PFU/ml) for use in therapeutic applications.

公开(公告)号：US12006517B2

公开(公告)日：2024-06-11

申请号：US17571645

申请日：2022-01-10

申请人： Adaptive Phage Therapeutics, Inc.

发明人： Joseph Robert Fackler ,  Carl Merril ,  Jarrar Haider ,  Viet Dang

IPC分类号： C12N7/00 ,  A61K35/76 ,  A61P31/04 ,  B01D61/20 ,  B01D69/08

CPC分类号： C12N7/00 ,  A61K35/76 ,  A61P31/04 ,  B01D61/20 ,  B01D69/08 ,  B01D2311/2676 ,  B01D2311/2688 ,  B01D2315/10 ,  C12N2795/10132 ,  C12N2795/10151 ,  C12N2795/10232 ,  C12N2795/10251 ,  C12N2795/10332 ,  C12N2795/10351

摘要： A method of recovering viable phage from, for example, a crude phage preparation such as a lysate resulting from amplification of phage in bacterial cell culture is disclosed. The method may be “universal”; that is, applicable to the purification of a broad range of phage species and strains. The phage product resulting from the method may have an acceptably low endotoxin titer (e.g. less than 500 EU/ml) and sufficiently high phage titer (e.g. >1×109 PFU/ml) for use in therapeutic applications.

公开(公告)号：US09357785B2

公开(公告)日：2016-06-07

申请号：US14057851

申请日：2013-10-18

申请人： The Texas A&M University System

发明人： Carlos F. Gonzalez ,  Stephen J. Ahern ,  Mayukh Das ,  Ryland F. Young, III ,  Tushar Surva Bhowmick

IPC分类号： A01N63/00 ,  C12R1/91 ,  C12N7/00

CPC分类号： A01N63/00 ,  C12N7/00 ,  C12N2795/10221 ,  C12N2795/10231 ,  C12N2795/10233 ,  C12N2795/10251 ,  C12N2795/10321 ,  C12N2795/10331 ,  C12R1/91

摘要： The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.

摘要翻译： 本发明提供了用于开发用于控制由Xylella fastidiosa引起的植物病害的基于有毒性噬菌体的处理的方法。 本发明进一步提供了在黄单胞菌属细菌宿主中分离和繁殖毒蕈毒素至十字戟科的方法，并用于治疗或减少植物中快速疟原虫感染的症状。 本发明进一步提供了分离和繁殖毒性至黄单胞菌属（Xanthomonas axonopodis pv）的方法。 柠檬酸和用于治疗或减轻黄单胞菌属的症状。 柑橘感染在植物中。

公开(公告)号：US09234227B2

公开(公告)日：2016-01-12

申请号：US13627060

申请日：2012-09-26

申请人： Sample6 Technologies, Inc.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12N15/00 ,  C12P19/34 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。

公开(公告)号：US20130122549A1

公开(公告)日：2013-05-16

申请号：US13627060

申请日：2012-09-26

申请人： SAMPLE6 TECHNOLOGIES, INC.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12P19/34

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。