公开(公告)号：US09828625B2

公开(公告)日：2017-11-28

申请号：US14309389

申请日：2014-06-19

申请人： Sample6 Technologies, Inc.

发明人： Michael Sandor Koeris ,  Julie Thomason ,  Michael Cappillino ,  Robert Patrick Shivers ,  Daniel Robert Brownell ,  Jayson L. Bowers ,  Timothy Kuan Ta Lu ,  Edyta Krzymanska-Olejnik

IPC分类号： C12Q1/66 ,  C12Q1/10 ,  C12N7/00 ,  G01N33/82

CPC分类号： C12Q1/66 ,  C12N7/00 ,  C12Q1/10 ,  G01N33/82 ,  G01N2333/245 ,  G01N2333/255

摘要： Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

公开(公告)号：US20160122729A1

公开(公告)日：2016-05-05

申请号：US14990274

申请日：2016-01-07

申请人： Sample6 Technologies, Inc.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making aa plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。

公开(公告)号：US09234227B2

公开(公告)日：2016-01-12

申请号：US13627060

申请日：2012-09-26

申请人： Sample6 Technologies, Inc.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12N15/00 ,  C12P19/34 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。

公开(公告)号：US20130122549A1

公开(公告)日：2013-05-16

申请号：US13627060

申请日：2012-09-26

申请人： SAMPLE6 TECHNOLOGIES, INC.

发明人： Timothy Kuan Ta Lu ,  Michael Sandor Koeris ,  Brett Smith Chevalier ,  Jason Wyatt Holder ,  Gregory John McKenzie ,  Daniel Robert Brownell

IPC分类号： C12P19/34

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。