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1.发明申请METHODS OF MAKING RECOMBINANT PHAGE, COMPOSITIONS AND ARTICLES OF MANUFACTURE OF SAME FOR BACTERIAL DETECTION 审中-公开制备重组蛋白的方法，组合物及其制备方法用于细菌检测公开(公告)号：US20170044502A1
公开(公告)日：2017-02-16
申请号：US15087151
申请日：2016-03-31
发明人： Michael Sandor Koeris , Robert Patrick Shivers , Daniel Robert Brownell , Jason Wyatt Holder , Jayson Linn Bowers
CPC分类号： C12N7/00 , C12N2795/00031 , C12Q1/04
摘要： Methods of detecting target bacteria are provided. In some embodiments, the methods comprise exposing a sample to a phage capable of infecting target bacteria, said phage comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments, the target bacteria comprise Listeria. In some embodiments, the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
摘要翻译： 提供了检测靶细菌的方法。 在一些实施方案中，所述方法包括将样品暴露于能够感染靶细菌的噬菌体，所述噬菌体包含编码标记的异源核酸序列。 在一些实施方案中，靶细菌包括李斯特菌。 在一些实施方案中，靶细菌都是李斯特菌。 还提供了包含编码标志物的异源核酸序列的重组李斯特氏菌噬菌体，作为包含这种噬菌体的这种噬菌体和制品的有用组合等。
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公开(公告)号：US09234227B2
公开(公告)日：2016-01-12
申请号：US13627060
申请日：2012-09-26
发明人： Timothy Kuan Ta Lu , Michael Sandor Koeris , Brett Smith Chevalier , Jason Wyatt Holder , Gregory John McKenzie , Daniel Robert Brownell
CPC分类号： C12N7/00 , C12N15/10 , C12N15/81 , C12N15/86 , C12N2795/10143 , C12N2795/10221 , C12N2795/10243 , C12N2795/10251 , C12N2800/206 , C12P19/34
摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。
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公开(公告)号：US20130122549A1
公开(公告)日：2013-05-16
申请号：US13627060
申请日：2012-09-26
发明人： Timothy Kuan Ta Lu , Michael Sandor Koeris , Brett Smith Chevalier , Jason Wyatt Holder , Gregory John McKenzie , Daniel Robert Brownell
IPC分类号： C12P19/34
CPC分类号： C12N7/00 , C12N15/10 , C12N15/81 , C12N15/86 , C12N2795/10143 , C12N2795/10221 , C12N2795/10243 , C12N2795/10251 , C12N2800/206 , C12P19/34
摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。
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4.发明授权Methods of making recombinant phage, compositions and articles of manufacture of same for bacterial detection 有权制备重组噬菌体，组合物及其制品的方法用于细菌检测公开(公告)号：US09340817B2
公开(公告)日：2016-05-17
申请号：US14226889
申请日：2014-03-27
发明人： Michael Sandor Koeris , Robert Patrick Shivers , Daniel Robert Brownell , Jason Wyatt Holder , Jayson Linn Bowers
CPC分类号： C12N7/00 , C12N2795/00031 , C12Q1/04
摘要： Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
摘要翻译： 提供了检测靶细菌的方法。 在一些实施方案中，所述方法包括将样品暴露于能够感染一组靶细菌并包含编码标记的异源核酸序列的噬菌体。 在一些实施方案中，靶细菌包括利斯特氏菌。 在一些实施方案中，靶细菌都是李斯特菌。 还提供了包含编码标志物的异源核酸序列的重组李斯特氏菌噬菌体，作为包含这样的噬菌体的这种噬菌体和制品的有用组合等。
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公开(公告)号：US20160122729A1
公开(公告)日：2016-05-05
申请号：US14990274
申请日：2016-01-07
发明人： Timothy Kuan Ta Lu , Michael Sandor Koeris , Brett Smith Chevalier , Jason Wyatt Holder , Gregory John McKenzie , Daniel Robert Brownell
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N15/10 , C12N15/81 , C12N15/86 , C12N2795/10143 , C12N2795/10221 , C12N2795/10243 , C12N2795/10251 , C12N2800/206 , C12P19/34
摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making aa plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
摘要翻译： 本公开提供了克隆噬菌体基因组的方法。 还提供了制备重组噬菌体基因组的方法。 在一些实施方案中，将噬菌体基因组工程化以包含异源核酸序列，例如包含开放阅读框的序列。 在一些实施方案中，噬菌体基因组克隆在酵母人造染色体中。 还提供了重组噬菌体基因组和重组噬菌体。 在一些实施方案中，所述方法是高通量方法，例如制备多个重组噬菌体基因组或重组噬菌体的方法。 还提供了重组噬菌体基因组和重组噬菌体的集合。
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公开(公告)号：US20140302487A1
公开(公告)日：2014-10-09
申请号：US14226889
申请日：2014-03-27
发明人： Michael Sandor Koeris , Robert Patrick Shivers , Daniel Robert Brownell , Jason Wyatt Holder , Jayson Linn Bowers
IPC分类号： C12Q1/04
CPC分类号： C12N7/00 , C12N2795/00031 , C12Q1/04
摘要： Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
摘要翻译： 提供了检测靶细菌的方法。 在一些实施方案中，所述方法包括将样品暴露于能够感染一组靶细菌并包含编码标记的异源核酸序列的噬菌体。 在一些实施方案中，靶细菌包括利斯特氏菌。 在一些实施方案中，靶细菌都是李斯特菌。 还提供了包含编码标志物的异源核酸序列的重组李斯特氏菌噬菌体，作为包含这种噬菌体的这种噬菌体和制品的有用组合等。
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