公开(公告)号：US09637787B2

公开(公告)日：2017-05-02

申请号：US14477224

申请日：2014-09-04

申请人： The Trustees of the University of Pennsylvania

发明人： Anil K. Rustgi

IPC分类号： C12Q1/68 ,  C12N5/071 ,  G01N33/50 ,  G01N33/543

CPC分类号： C12Q1/6876 ,  C12N5/0679 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/40 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2502/1323 ,  C12N2502/23 ,  C12N2502/30 ,  C12N2503/04 ,  C12N2533/54 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/5044 ,  G01N33/54306 ,  G01N2333/4706

摘要： A 3D organotypic culture which phenocopies aggressive, invasive cancer and methods of use thereof are provided.

公开(公告)号：US08858631B2

公开(公告)日：2014-10-14

申请号：US13542202

申请日：2012-07-05

申请人： Alexander M. Seifalian

发明人： Alexander M. Seifalian

IPC分类号： A61F2/20 ,  A61F2/04 ,  A61L27/18 ,  A61L27/38 ,  A61F2/02 ,  A61L27/54 ,  A61L27/36

CPC分类号： A61F2/04 ,  A61F2/02 ,  A61F2002/046 ,  A61F2230/006 ,  A61F2250/0029 ,  A61L27/18 ,  A61L27/20 ,  A61L27/3679 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3882 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/412 ,  A61L2300/414 ,  A61L2300/64 ,  A61L2400/12 ,  A61L2430/22 ,  C12N5/0663 ,  C12N2502/23 ,  C12N2533/40 ,  C08L75/04

摘要： Articles, compositions, and methods for growing tissues and organs using bioreactors, including rotating bioreactors, are provided. Synthetic scaffolds for growing artificial tissue and organ transplants are also provided.

摘要翻译： 提供了使用生物反应器（包括旋转生物反应器）生长组织和器官的制品，组合物和方法。 还提供用于生长人造组织和器官移植物的合成支架。

公开(公告)号：US20130302888A1

公开(公告)日：2013-11-14

申请号：US13978764

申请日：2012-02-17

申请人： Frederic Demarne ,  Vincent Jannin ,  Alf Lamprecht ,  Yann Pellequer ,  Arnaud Beduneau

发明人： Frederic Demarne ,  Vincent Jannin ,  Alf Lamprecht ,  Yann Pellequer ,  Arnaud Beduneau

IPC分类号： C12N5/071

CPC分类号： C12N5/0679 ,  C12N2502/23 ,  C12N2503/02 ,  C12N2503/04 ,  G01N33/5008

摘要： Cell culture process includes seeding a suitable culture medium with enterocytes and then, after a delay, seeding the medium containing the enterocytes that have begun to proliferate, with goblet cells.

摘要翻译： 细胞培养过程包括将合适的培养基与肠细胞接种，然后延迟地将含有开始增殖的肠细胞的培养基与杯状细胞接种。

公开(公告)号：US20110053185A1

公开(公告)日：2011-03-03

申请号：US12771683

申请日：2010-04-30

申请人： Anil K. Rustgi ,  Carmen Michaylira ,  Douglas B. Stairs

发明人： Anil K. Rustgi ,  Carmen Michaylira ,  Douglas B. Stairs

IPC分类号： G01N33/574 ,  C12N5/077 ,  G01N33/53

CPC分类号： C12Q1/6876 ,  C12N5/0679 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/40 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2502/1323 ,  C12N2502/23 ,  C12N2502/30 ,  C12N2503/04 ,  C12N2533/54 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/5044 ,  G01N33/54306 ,  G01N2333/4706

摘要： A 3D organotypic culture which phenocopies aggressive, invasive cancer and methods of use thereof are provided.

摘要翻译： 提供了具有侵略性侵袭性侵袭性癌症及其使用方法的3D器官型培养物。

公开(公告)号：US20090304645A1

公开(公告)日：2009-12-10

申请号：US12456377

申请日：2009-06-16

申请人： Bradley P. Kropp ,  Earl Y. Cheng ,  Yuan Yuan Zhang ,  Rick Cowan ,  Peter Moore

发明人： Bradley P. Kropp ,  Earl Y. Cheng ,  Yuan Yuan Zhang ,  Rick Cowan ,  Peter Moore

IPC分类号： A61K35/12 ,  A61P43/00

CPC分类号： C12N5/0684 ,  A61F2/02 ,  A61K35/12 ,  C12N2502/1347 ,  C12N2502/23 ,  C12N2533/92

摘要： A method for providing a urinary tract tissue graft composition includes providing a tissue culture frame and a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in the tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. Smooth muscle and urothelial cells are isolated from a tissue specimen of a subject and cultured, and then seeded upon the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. A tissue culture frame in which such a urinary tract tissue graft may be formed is also disclosed.

公开(公告)号：US07122200B2

公开(公告)日：2006-10-17

申请号：US10013270

申请日：2001-12-10

申请人： Bradley P. Kropp ,  Earl Y. Cheng ,  Yuan Yuan Zhang ,  Rick Cowan ,  Peter Moore

发明人： Bradley P. Kropp ,  Earl Y. Cheng ,  Yuan Yuan Zhang ,  Rick Cowan ,  Peter Moore

IPC分类号： A61F13/00 ,  A01N65/00 ,  C12N5/08

CPC分类号： C12N5/0684 ,  A61F2/02 ,  A61K35/12 ,  C12N2502/1347 ,  C12N2502/23 ,  C12N2533/92

摘要： A method for providing a urinary tract tissue graft composition includes providing a tissue culture frame and a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in the tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. Smooth muscle and urothelial cells are isolated from a tissue specimen of a subject and cultured, and then seeded upon the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. A tissue culture frame in which such a urinary tract tissue graft may be formed is also disclosed.

摘要翻译： 提供尿道组织移植组合物的方法包括提供组织培养框架和小肠粘膜下层的一部分，并将小肠粘膜下层的部分定位在组织培养框架中，使得小肠粘膜下层的部分被悬浮并保持在 由组织培养框架拉紧位置。 从受试者的组织标本中分离平滑肌和尿路上皮细胞并培养，然后接种在小肠粘膜下层的段上，从而形成尿道组织移植物。 还公开了可以形成这种尿道组织移植物的组织培养框架。

公开(公告)号：US12115195B2

公开(公告)日：2024-10-15

申请号：US17363898

申请日：2021-06-30

申请人： INCUBE LABS, LLC

发明人： Mir Imran

IPC分类号： A61K35/38 ,  A61K35/28 ,  C12N5/071

CPC分类号： A61K35/38 ,  A61K35/28 ,  C12N5/0679 ,  C12N2502/025 ,  C12N2502/03 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/23 ,  C12N2506/1346 ,  C12N2506/45 ,  C12N5/0679 ,  C12N2502/1352 ,  C12N5/0679 ,  C12N2502/03 ,  C12N5/0679 ,  C12N2506/1346 ,  C12N5/0679 ,  C12N2506/03

摘要： Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.

公开(公告)号：US20170335276A1

公开(公告)日：2017-11-23

申请号：US15538184

申请日：2015-12-23

申请人： INSTITUT PASTEUR ,  FONDATION IMAGINE ,  ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS ,  UNIVERSITE PARIS DESCARTES ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

发明人： EBERL Gerard ,  BIKARD David ,  SCHNUPF Pamela ,  CERF BENSUSSAN Nadine ,  GABORIAU-ROUTHIAU Valerie ,  SANSONETTI Philippe

IPC分类号： C12N1/20 ,  C12N13/00

CPC分类号： C12N1/20 ,  C12N13/00 ,  C12N15/74 ,  C12N2502/23

摘要： The present invention relates to an in vitro method of culturing a segmented filamentous bacterium strain, comprising co-culturing said segmented filamentous bacterium strain with a eukaryotic host cell, wherein the culture is performed at an O2 level inferior to 5% in a rich tissue culture liquid medium containing bacterial medium components including iron. The present invention also relates to methods for genetically modifying a segmented filamentous bacterium strain comprising a step a culturing the strain in vitro.

公开(公告)号：US20160068805A1

公开(公告)日：2016-03-10

申请号：US14853564

申请日：2015-09-14

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  THE UNITED STATES GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS

发明人： Martin G. Martin ,  James C.Y. Dunn ,  Matthias Stelzner

IPC分类号： C12N5/071 ,  A61K35/38

CPC分类号： C12N5/068 ,  A61K35/38 ,  A61K48/00 ,  C12N5/0679 ,  C12N2501/11 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2502/23

摘要： A cell culture media composition for support growth of human SI stem cells and epithelium without a feeder layer is presented. The media may also include growth factors including ENR and Y-27632 that support the survival of stem cell spheroid structures. The cell culture media compositions permit rapid growth of human small intestinal (SI) epithelium and stem cells, which leads to specific spheroid cell and enteroid morphology when grown in 3-D culture system.

摘要翻译： 提出了用于支持人SI干细胞和没有饲养层的上皮的生长的细胞培养基组合物。 培养基还可能包括生长因子，包括支持干细胞球体结构存活的ENR和Y-27632。 细胞培养基组合物允许人类小肠（SI）上皮细胞和干细胞的快速生长，当在3-D培养系统中生长时，其导致特定的球形细胞和肠形成形态。

公开(公告)号：US20160040131A1

公开(公告)日：2016-02-11

申请号：US14833403

申请日：2015-08-24

申请人： Incube Labs LLC

发明人： Mir Imran

IPC分类号： C12N5/071 ,  A61K35/38

CPC分类号： C12N5/0679 ,  A61K35/38 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/23 ,  C12N2506/1346 ,  C12N2506/1353

摘要： Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.

摘要翻译： 本发明的各种实施方案提供了治疗糖尿病和其它葡萄糖调节障碍的方法。 在一个实施方案中，该方法包括从供体中除去L-细胞，从患者获得干细胞，并在干细胞存在下培养L细胞，使得干细胞分化成干细胞衍生的L-细胞， 细胞（SCDLC）。 将足够的SCDLC量引入到患者摄取食物后降低患者的血糖水平。 在另一个实施方案中，该方法包括从供体中除去K细胞，从患者获得干细胞，并在干细胞存在下培养K细胞，使得干细胞分化成干细胞衍生的K- 细胞（SCDKC）。 将足够的SCDKC的量引入到患者摄取食物后使患者的血糖水平降低。