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公开(公告)号：US20080101988A1
公开(公告)日：2008-05-01
申请号：US11314174
申请日：2005-12-21
Applicant: Xiaofeng Kang , Li Qun Gu , Stephen Cheley , Hagan Bayley
Inventor： Xiaofeng Kang , Li Qun Gu , Stephen Cheley , Hagan Bayley
IPC: G01N21/00
CPC classification number: G01N33/54366 , G01N33/48721 , G01N33/54393 , G01N33/6872
Abstract: The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55° C. and even as high as near 100° C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.
Abstract translation: 本发明包括用于将分析物随机感测到蛋白质孔的装置，系统和方法。 蛋白质孔可以是工程蛋白孔，例如在高于55℃的温度下甚至高达100℃的离子通道。分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的电流信号。 可能的信号包括电流变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 也可以检测存在于相同溶液中的多种分析物。
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公开(公告)号：US07867716B2
公开(公告)日：2011-01-11
申请号：US11314174
申请日：2005-12-21
Applicant: Xiaofeng Kang , Li Qun Gu , Stephen Cheley , Hagan Bayley
Inventor： Xiaofeng Kang , Li Qun Gu , Stephen Cheley , Hagan Bayley
IPC: G01N33/53
CPC classification number: G01N33/54366 , G01N33/48721 , G01N33/54393 , G01N33/6872
Abstract: The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55° C. and even as high as near 100° C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.
Abstract translation: 本发明包括用于将分析物随机感测到蛋白质孔的装置，系统和方法。 蛋白质孔可以是工程蛋白孔，例如在高于55℃的温度下甚至高达100℃的离子通道。分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的电流信号。 可能的信号包括电流变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 也可以检测存在于相同溶液中的多种分析物。
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公开(公告)号：US20070298511A1
公开(公告)日：2007-12-27
申请号：US11788293
申请日：2007-04-19
Applicant: Xiaofeng Kang , Stephen Cheley , Hagan Bayley
Inventor： Xiaofeng Kang , Stephen Cheley , Hagan Bayley
CPC classification number: G01N33/48721
Abstract: The invention relates to a nanopore sensor system including methods of fabrication and uses disclosed herein. In some embodiments, the invention relates to a substrate comprising a lipid membrane, preferably a phospholipid bilayer film, having a nanopore and a gel surrounding said lipid membrane. In additional embodiments, the invention relates to compositions and methods of using and making a substrate that has a lipid membrane having a single channel protein surrounded with a gel. In further embodiments, the invention relates to a method of detecting an analyte by mixing a nanopore sensor with a solution suspected of containing an analyte, measuring electrical properties, and correlating changes of electrical properties to the existence of an analyte.
Abstract translation: 本发明涉及包括本文公开的制造方法和用途的纳米孔传感器系统。 在一些实施方案中，本发明涉及包含脂质膜，优选磷脂双层膜的底物，其具有纳米孔和围绕所述脂质膜的凝胶。 在另外的实施方案中，本发明涉及使用和制备具有由凝胶包围的单通道蛋白质的脂质膜的底物的组合物和方法。 在另外的实施方案中，本发明涉及一种通过将纳米孔传感器与疑似含有分析物的溶液混合来检测分析物的方法，测量电性能，以及将电特性的变化与分析物的存在相关联。
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公开(公告)号：US08404449B2
公开(公告)日：2013-03-26
申请号：US12429897
申请日：2009-04-24
Applicant: Hagan Bayley , Seong-Ho Shin , Tudor Luchian , Stephen Cheley
Inventor： Hagan Bayley , Seong-Ho Shin , Tudor Luchian , Stephen Cheley
IPC: G01N33/53
CPC classification number: G01N33/5308 , C12Q1/001 , G01N33/5438 , G01N33/6872
Abstract: A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or αHL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.
Abstract translation: 用于随机感测的系统和方法，其中分析物共价键合到传感器元件或适配器元件。 如果这种键合是不可逆的，则该键可能被化学试剂破坏。 传感器元件可以是蛋白质，例如工程化的PSH型或αHL蛋白质孔。 分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的信号。 可能的信号包括电流的变化，力的变化和荧光的变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 可以检测存在于相同溶液中的多种分析物。
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公开(公告)号：US20090283422A1
公开(公告)日：2009-11-19
申请号：US12429897
申请日：2009-04-24
Applicant: Hagan Bayley , Seong-Ho Shin , Tudor Luchian , Stephen Cheley
Inventor： Hagan Bayley , Seong-Ho Shin , Tudor Luchian , Stephen Cheley
IPC: G01N27/26
CPC classification number: G01N33/5308 , C12Q1/001 , G01N33/5438 , G01N33/6872
Abstract: A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or αHL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.
Abstract translation: 用于随机感测的系统和方法，其中分析物共价键合到传感器元件或适配器元件。 如果这种键合是不可逆的，则该键可能被化学试剂破坏。 传感器元件可以是蛋白质，例如工程化的PSH型或αHL蛋白质孔。 分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的信号。 可能的信号包括电流的变化，力的变化和荧光的变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 可以检测存在于相同溶液中的多种分析物。
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Patent Agency Ranking
公开(公告)号：US20110229877A1
公开(公告)日：2011-09-22
申请号：US13002709
申请日：2009-07-06
Applicant: Lakmal Jayasinghe , Hagan Bayley , Stephen Cheley , Brian Mckeown , James White , James Clarke
Inventor： Lakmal Jayasinghe , Hagan Bayley , Stephen Cheley , Brian Mckeown , James White , James Clarke
CPC classification number: C12Q1/6869 , C07K14/31 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12N9/16 , C12N9/22 , C12N9/52 , C12N9/90 , C12N9/96 , C12Q2565/631
Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
Abstract translation: 本发明涉及包含跨膜蛋白质孔亚基和核酸处理酶的构建体。 孔亚基共价连接到酶上，使得亚基和酶保持其活性。 构建体可用于产生具有附着于其上的核酸处理酶的跨膜蛋白孔。 这样的孔特别可用于测序核酸。 酶以这样的方式处理核酸，使得孔可以通过随机感测来检测其成分核苷酸。
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公开(公告)号：US06927070B1
公开(公告)日：2005-08-09
申请号：US10180792
申请日：2002-06-25
Applicant: Hagan Bayley , Orit Braha , LiQun Gu
Inventor： Hagan Bayley , Orit Braha , LiQun Gu
IPC: G01N33/543
CPC classification number: G01N33/54393 , G01N33/54366 , Y10S435/808 , Y10S436/805 , Y10S436/806
Abstract: This invention relates to an improved method and system for sensing of one or more analytes. A host molecule, which serves as an adapter/carrier, is used to facilitate interaction between the analyte and the sensor element. A detectable signal is produced reflecting the identity and concentration of analyte present.
Abstract translation: 本发明涉及用于感测一种或多种分析物的改进的方法和系统。 用作适配器/载体的宿主分子用于促进分析物和传感器元件之间的相互作用。 产生可检测的信号，反映存在的分析物的身份和浓度。
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公开(公告)号：US06824659B2
公开(公告)日：2004-11-30
申请号：US09784985
申请日：2001-02-15
Applicant: Hagan Bayley , Orit Braha , John Kasianowicz , Eric Gouaux
Inventor： Hagan Bayley , Orit Braha , John Kasianowicz , Eric Gouaux
IPC: G01N27327
CPC classification number: C07K14/31 , G01N33/48721
Abstract: A mutant staphylococcal alpha hemolysin polypeptide containing a heterologous analyte-binding amino acid which assembles into an analyte-responsive heptameric pore assembly in the presence of a wild type staphylococcal alpha hemolysin polypeptide, digital biosensors, and methods of detecting, identifying, and quantifying analytes are described.
Abstract translation: 在野生型葡萄球菌α溶血素多肽，数字生物传感器以及检测，鉴定和定量分析物的方法中，含有异源分析物结合氨基酸的突变型葡萄球菌α溶血素多肽组装成分析物响应性七聚体孔组件， 描述。
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公开(公告)号：US09963518B2
公开(公告)日：2018-05-08
申请号：US14239826
申请日：2012-08-20
Applicant: Lingbing Kong , Benjamin G. Davis , Hagan Bayley
Inventor： Lingbing Kong , Benjamin G. Davis , Hagan Bayley
IPC: A61K31/724 , C08B37/16 , A01N43/16 , A61K47/40
CPC classification number: C08B37/0012 , A01N43/16 , A61K31/724 , A61K47/40 , Y02A50/473
Abstract: Compounds which inhibit Wza-mcdiated polysaccharide transport are useful in the prevention or treatment of bacterial infection, in particular of E. coli infection. The compounds are typically cyclic oligosaccharides such as cyclodextrins, which bear positively charged functional groups at the primary carbon position.
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公开(公告)号：US08785211B2
公开(公告)日：2014-07-22
申请号：US13338794
申请日：2011-12-28
Applicant: Hagan Bayley , Yann Astier , Orit Braha
Inventor： Hagan Bayley , Yann Astier , Orit Braha
IPC: C12Q1/68 , G01N33/543 , G01N27/26
CPC classification number: G01N33/48721 , C12Q1/6869 , C12Q2565/631
Abstract: The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
Abstract translation: 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。
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