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公开(公告)号：US08105846B2
公开(公告)日：2012-01-31
申请号：US12093610
申请日：2006-11-15
申请人： Hagan Bayley , Yann Astier , Orit Braha
发明人： Hagan Bayley , Yann Astier , Orit Braha
IPC分类号： G01N33/543 , G01N27/26
CPC分类号： G01N33/48721 , C12Q1/6869 , C12Q2565/631
摘要： The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
摘要翻译： 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。
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公开(公告)号：US20080311582A1
公开(公告)日：2008-12-18
申请号：US12093610
申请日：2006-11-15
申请人： Hagan Bayley , Yann Astier , Orit Braha
发明人： Hagan Bayley , Yann Astier , Orit Braha
IPC分类号： C12Q1/68
CPC分类号： G01N33/48721 , C12Q1/6869 , C12Q2565/631
摘要： The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
摘要翻译： 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。
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公开(公告)号：US08785211B2
公开(公告)日：2014-07-22
申请号：US13338794
申请日：2011-12-28
申请人： Hagan Bayley , Yann Astier , Orit Braha
发明人： Hagan Bayley , Yann Astier , Orit Braha
IPC分类号： C12Q1/68 , G01N33/543 , G01N27/26
CPC分类号： G01N33/48721 , C12Q1/6869 , C12Q2565/631
摘要： The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
摘要翻译： 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。
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公开(公告)号：US20100297638A1
公开(公告)日：2010-11-25
申请号：US12681643
申请日：2008-10-06
申请人： John Hagan Bayley , Haichen Wu , Giovanni Maglia , Yann Astier
发明人： John Hagan Bayley , Haichen Wu , Giovanni Maglia , Yann Astier
CPC分类号： G01N33/6872 , C12Q1/6869 , Y10T436/147777 , C12Q2565/631 , C12Q2537/157 , C12Q2521/325
摘要： The invention relates to transmembrane protein pore for use in detecting a analyte in a sample. The pore comprises a molecular adaptor that facilitates an interaction between the pore and the analyte. The adaptor is covalently attached to the pore in an orientation that allows the analyte to be detected using the pore.
摘要翻译： 本发明涉及用于检测样品中分析物的跨膜蛋白质孔。 孔包含促进孔和分析物之间的相互作用的分子衔接子。 适配器以允许使用孔检测分析物的方向共价连接到孔。
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公开(公告)号：US08822160B2
公开(公告)日：2014-09-02
申请号：US12681643
申请日：2008-10-06
申请人： John Hagan Bayley , Haichen Wu , Giovanni Maglia , Yann Astier
发明人： John Hagan Bayley , Haichen Wu , Giovanni Maglia , Yann Astier
CPC分类号： G01N33/6872 , C12Q1/6869 , Y10T436/147777 , C12Q2565/631 , C12Q2537/157 , C12Q2521/325
摘要： The invention relates to transmembrane protein pore for use in detecting a analyte in a sample. The pore comprises a molecular adaptor that facilitates an interaction between the pore and the analyte. The adaptor is covalently attached to the pore in an orientation that allows the analyte to be detected using the pore.
摘要翻译： 本发明涉及用于检测样品中分析物的跨膜蛋白质孔。 孔包含促进孔和分析物之间的相互作用的分子衔接子。 适配器以允许使用孔检测分析物的方向共价连接到孔。
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公开(公告)号：US20050178674A1
公开(公告)日：2005-08-18
申请号：US10499129
申请日：2002-12-23
申请人： Mark Hyland , Kevin Lorimer , Ronald Butler , Emma Wallace-Davies , Yann Astier
发明人： Mark Hyland , Kevin Lorimer , Ronald Butler , Emma Wallace-Davies , Yann Astier
IPC分类号： G01N27/327 , G01N27/403 , G01N27/413 , G01N27/416 , G01N27/49 , G01N27/26
CPC分类号： G01N27/3272 , Y10T29/49002
摘要： The invention concerns an electrochemical cell which, either alone or together which a substrate onto which it is placed, is in the form of a receptacle. The electrochemical cell contains a working electrode and a counter electrode, the working electrode being in a wall of the receptacle. At least one of the electrodes has at least one dimension of less than 50 μm. The electrochemical cell is principally intended for use as a micro-electrode suitable for screening water, blood, urine or other biological or non-biological fluids.
摘要翻译： 本发明涉及一种电化学电池，其单独地或一起放置有放置在其上的衬底，其为容器的形式。 电化学电池包含工作电极和对电极，工作电极位于容器的壁中。 至少一个电极具有小于50μm的至少一个尺寸。 电化学电池主要用作适用于筛选水，血液，尿液或其他生物或非生物流体的微电极。
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公开(公告)号：US20050164329A1
公开(公告)日：2005-07-28
申请号：US10513443
申请日：2003-05-16
申请人： Emma Wallace-Davis , Yann Astier
发明人： Emma Wallace-Davis , Yann Astier
IPC分类号： G01N27/26 , C12M1/34 , C12Q1/00 , C12Q1/26 , C12Q1/28 , C12Q1/32 , C12Q1/44 , C12Q1/48 , G01N27/327 , G01N27/416 , G01N27/48 , G01N33/487
CPC分类号： C12Q1/001
摘要： A method for determining the concentration of an analyte in a sample is disclosed which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.
摘要翻译： 公开了一种用于确定样品中分析物浓度的方法，其包括将样品与微电极接触，所述微电极包含能够与所述分析物反应的酶和氧化还原介体，所述氧化还原介体能够被所述氧化还原介质氧化或还原， 酶一旦后者与分析物反应，允许分析物与酶反应，然后在电极上施加电势，并电化学测量转化的介体的所得浓度。
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公开(公告)号：US11802870B1
公开(公告)日：2023-10-31
申请号：US18079986
申请日：2022-12-13
申请人： Yann Astier , Juraj Topolancik
发明人： Yann Astier , Juraj Topolancik
IPC分类号： G01N33/557 , G01N33/543
CPC分类号： G01N33/557 , G01N33/54313 , G01N2470/04
摘要： A microfluidic chip with an array of pillars for directing flow of beads is used to measure reaction kinetics. A stream may be continuously drawn from the reaction volume into the microfluidic chip. The bead is attached to a primary antibody. The reaction volume has an antigen and a second antibody with a label. The primary antibody binds to the antigen, and the secondary antibody binds to the antigen, creating a sandwich of bead, antigen, and label. The binding reactions occur over time in the reaction volume. The beads may be imaged after traversing a laminar wash buffer, and the signal intensity is measured. Each bead provides a kinetic monitoring of the immunoassay over the reaction time at which the bead is removed from the reaction media. Methods and systems are described in this disclosure.
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公开(公告)号：US07534583B2
公开(公告)日：2009-05-19
申请号：US10513443
申请日：2003-05-16
IPC分类号： C12Q1/26
CPC分类号： C12Q1/001
摘要： A method for determining the concentration of an analyte in a sample is disclosed which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.
摘要翻译： 公开了一种用于确定样品中分析物浓度的方法，其包括将样品与微电极接触，所述微电极包含能够与所述分析物反应的酶和氧化还原介体，所述氧化还原介体能够被所述氧化还原介质氧化或还原， 酶一旦后者与分析物反应，允许分析物与酶反应，然后在电极上施加电势，并电化学测量转化的介体的所得浓度。
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公开(公告)号：US07972487B2
公开(公告)日：2011-07-05
申请号：US10499129
申请日：2002-12-23
申请人： Mark Hyland , Kevin Lorimer , Ronald Neil Butler , Emma Naomi Kathlene Wallace-Davis , Yann Astier
发明人： Mark Hyland , Kevin Lorimer , Ronald Neil Butler , Emma Naomi Kathlene Wallace-Davis , Yann Astier
CPC分类号： G01N27/3272 , Y10T29/49002
摘要： The invention concerns an electrochemical cell which, either alone or together which a substrate onto which it is placed, is in the form of a receptacle. The electrochemical cell contains a working electrode and a counter electrode, the working electrode being in a wall of the receptacle. At least one of the electrodes has at least one dimension of less than 50 μm. The electrochemical cell is principally intended for use as a micro-electrode suitable for screening water, blood, urine or other biological or non-biological fluids.
摘要翻译： 本发明涉及一种电化学电池，其单独地或一起放置有放置在其上的衬底，其为容器的形式。 电化学电池包含工作电极和对电极，工作电极位于容器的壁中。 至少一个电极具有小于50μm的至少一个尺寸。 电化学电池主要用作适用于筛选水，血液，尿液或其他生物或非生物流体的微电极。
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