公开(公告)号：US20240326019A1

公开(公告)日：2024-10-03

申请号：US18739559

申请日：2024-06-11

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche ,  Andrew Wyatt Schmudlach ,  Matthew A. Lauber

IPC: B01J20/289 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28

CPC classification number: B01J20/289 ,  B01J20/103 ,  B01J20/262 ,  B01J20/265 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/28083 ,  B01J20/28085 ,  B01J2220/4806 ,  B01J2220/54 ,  B01J2220/58

Abstract: The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.

公开(公告)号：US12138617B2

公开(公告)日：2024-11-12

申请号：US17477347

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Nicole L. Lawrence ,  Yeliz Tunc Sarisozen ,  Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: B01J20/289 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

公开(公告)号：US20240361318A1

公开(公告)日：2024-10-31

申请号：US18647863

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Martin Gilar ,  Yeliz Tunc Sarisozen

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543 ,  G01N33/74

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/74 ,  G01N2333/015 ,  G01N2333/62 ,  G01N2469/10

Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

公开(公告)号：US20220118443A1

公开(公告)日：2022-04-21

申请号：US17502483

申请日：2021-10-15

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew Lauber ,  Andrew Wyatt Schmudlach ,  Daniel P. Walsh ,  Yeliz Tunc Sarisozen ,  Jonathan Belanger ,  Darryl W. Brousmiche ,  Andrew Bates-Harrison ,  Nicole L. Lawrence

IPC: B01L3/00 ,  C09D143/04

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

公开(公告)号：US20220081468A1

公开(公告)日：2022-03-17

申请号：US17477331

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Andrew Wyatt Schmudlach ,  Susan Rzewuski ,  Matthew A. Lauber ,  Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche

IPC: C07K1/22 ,  C07C277/06 ,  C07C227/40 ,  B01J20/285 ,  B01J20/28 ,  B01J20/283 ,  B01J20/26 ,  B01J20/10 ,  C12N7/00 ,  B01D15/34 ,  B01J20/281

Abstract: The present disclosure is directed to methods for performing size exclusion chromatography. Embodiments of the present disclosure feature methods for improving separations of proteinaceous analytes in size exclusion chromatography, for example, by using low concentrations of amino acids or derivatives thereof in the mobile phase.

公开(公告)号：US20240359161A1

公开(公告)日：2024-10-31

申请号：US18647811

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  MingCheng Xu ,  Matthew Lauber

IPC: B01J20/289 ,  B01D15/22 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32

CPC classification number: B01J20/289 ,  B01D15/22 ,  B01D15/3809 ,  B01J20/261 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with streptavidin. The streptavidin functionalized particles of the present disclosure can be bound to biotinylated antibodies, biotinylated antigen-binding fragments thereof, or biotinylated oligonucleotides to create customizable affinity chromatography columns. That is, the functionalized nonporous polymer particles can be packed in a stainless steel, titanium, or other metal or metal alloy column for affinity high pressure liquid chromatography (HPLC).

公开(公告)号：US12036529B2

公开(公告)日：2024-07-16

申请号：US17477340

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L Lawrence ,  Darryl W. Brousmiche ,  Andrew Wyatt Schmudlach ,  Matthew A. Lauber

IPC: B01J20/28 ,  B01J20/10 ,  B01J20/26 ,  B01J20/289

CPC classification number: B01J20/289 ,  B01J20/103 ,  B01J20/262 ,  B01J20/265 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/28083 ,  B01J20/28085 ,  B01J2220/4806 ,  B01J2220/54 ,  B01J2220/58

Abstract: Stationary phase materials for performing size exclusion chromatography are provided. The stationary phase materials include porous inorganic-organic hybrid particles surface-modified with a hydroxy-terminated polyethylene glycol silane.

公开(公告)号：US20230182114A1

公开(公告)日：2023-06-15

申请号：US18056434

申请日：2022-11-17

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche ,  MingCheng Xu ,  Kevin Wyndham

IPC: B01J20/289 ,  B01J20/287 ,  B01J20/28 ,  B01J20/10 ,  B01D15/34

CPC classification number: B01J20/289 ,  B01D15/34 ,  B01J20/103 ,  B01J20/287 ,  B01J20/2808 ,  B01J20/28052 ,  B01J20/28083 ,  B01J20/28085 ,  B01J2220/54 ,  B01J2220/64 ,  B01J2220/66

Abstract: The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.

公开(公告)号：US12201975B2

公开(公告)日：2025-01-21

申请号：US17502483

申请日：2021-10-15

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew Lauber ,  Andrew Wyatt Schmudlach ,  Daniel P. Walsh ,  Yeliz Tunc Sarisozen ,  Jonathan Belanger ,  Darryl W. Brousmiche ,  Andrew Bates-Harrison ,  Nicole L. Lawrence

IPC: G01N30/56 ,  B01L3/00 ,  C09D143/04 ,  C23C16/56 ,  G01N30/60

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

公开(公告)号：US20240367072A1

公开(公告)日：2024-11-07

申请号：US18654384

申请日：2024-05-03

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  Mingcheng Xu ,  Matthew Lauber

IPC: B01D15/38 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with an immunoglobulin-binding protein. The functionalized particles of the present disclosure can be used to purify a range of immunoglobulins with affinity to the immunoglobulin-binding protein.