公开(公告)号：US20200347423A1

公开(公告)日：2020-11-05

申请号：US16878032

申请日：2020-05-19

申请人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12P19/32 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/46 ,  C12N9/04 ,  C12N9/02 ,  C12N9/00 ,  C12P7/62 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/42 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US11913049B2

公开(公告)日：2024-02-27

申请号：US16878032

申请日：2020-05-19

申请人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12N9/00 ,  C12P19/32 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/46 ,  C12N9/04 ,  C12N9/02 ,  C12P7/625 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/42 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/001 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US20220112477A1

公开(公告)日：2022-04-14

申请号：US17510641

申请日：2021-10-26

申请人： Ramon Gonzalez ,  Alexander Chou ,  James Clomburg

发明人： Ramon Gonzalez ,  Alexander Chou ,  James Clomburg

IPC分类号： C12N9/88 ,  C12N15/52 ,  C12P11/00 ,  C12P7/24 ,  C12P13/00 ,  C12P5/02 ,  C12P7/40 ,  C12P1/04 ,  C12P7/04 ,  C12N15/70 ,  C12P7/20

摘要： An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.

公开(公告)号：US11186834B2

公开(公告)日：2021-11-30

申请号：US15523284

申请日：2015-10-29

申请人： Ramon Gonzalez ,  Alexander Chou ,  James Clomburg

发明人： Ramon Gonzalez ,  Alexander Chou ,  James Clomburg

IPC分类号： C12P7/40 ,  C12P7/44 ,  C12P7/18 ,  C12N9/16 ,  C12N9/04 ,  C12N9/88 ,  C12N15/52 ,  C12P11/00 ,  C12P7/24 ,  C12P13/00 ,  C12P5/02 ,  C12P1/04 ,  C12P7/04 ,  C12N15/70 ,  C12P7/20

摘要： An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.

公开(公告)号：US09416364B2

公开(公告)日：2016-08-16

申请号：US13983885

申请日：2012-02-07

申请人： Ramon Gonzalez ,  James Clomburg ,  Clementina Dellomonaco ,  Elliot N. Miller

发明人： Ramon Gonzalez ,  James Clomburg ,  Clementina Dellomonaco ,  Elliot N. Miller

IPC分类号： C12N15/70 ,  C12P5/02 ,  C12P7/40 ,  C12P7/04 ,  C12P7/16 ,  C12P7/42 ,  C12P7/64

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/70 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P7/40 ,  C12P7/42 ,  C12P7/6409 ,  C12Y101/01035 ,  C12Y102/0105 ,  C12Y103/99003 ,  C12Y203/01 ,  C12Y402/01017 ,  Y02E50/10 ,  Y02P20/52

摘要： The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.

摘要翻译： 本发明涉及重组微生物，其已被工程改造成使用已被重新利用以产生反向β氧化途径的基因产生各种化学物质。 一般来说，通过根据需要修改多个循环的各种调节点，反向表达和驱动β氧化循环，然后通过终止酶的作用将CoA硫酯中间体转化成有用的产物。

公开(公告)号：US20130316413A1

公开(公告)日：2013-11-28

申请号：US13983885

申请日：2012-02-07

申请人： Ramon Gonzalez ,  James Clomburg ,  Clementina Dellomonaco ,  Elliot N. Miller

发明人： Ramon Gonzalez ,  James Clomburg ,  Clementina Dellomonaco ,  Elliot N. Miller

IPC分类号： C12N15/70

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/70 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P7/40 ,  C12P7/42 ,  C12P7/6409 ,  C12Y101/01035 ,  C12Y102/0105 ,  C12Y103/99003 ,  C12Y203/01 ,  C12Y402/01017 ,  Y02E50/10 ,  Y02P20/52

摘要： The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.

摘要翻译： 本发明涉及重组微生物，其已被工程改造成使用已被重新利用以产生反向β氧化途径的基因产生各种化学物质。 一般来说，通过根据需要修改多个循环的各种调节点，反向表达和驱动β氧化循环，然后通过终止酶的作用将CoA硫酯中间体转化为有用的产物。

公开(公告)号：US20240309417A1

公开(公告)日：2024-09-19

申请号：US18413954

申请日：2024-01-16

申请人： Ramon Gonzalez

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US20220162661A1

公开(公告)日：2022-05-26

申请号：US17537714

申请日：2021-11-30

申请人： Ramon Gonzalez

发明人： Ramon Gonzalez ,  Alexander Chou ,  James Clomburg

IPC分类号： C12P19/40

摘要： The production of substituted 2-hydroxyacyl-CoA molecules by a novel reaction is described. The reaction involves the condensation of formyl-CoA with a carbonyl-containing molecule. Such carbonyl-containing molecules include a substituted aldehyde and a ketone. The reaction is catalyzed by enzymes using a TPP-dependent mechanism. Also described is the production of unsubstituted and substituted 2-hydroxyacyl-CoA molecules comprising the condensation of formyl-CoA with a carbonyl-containing molecule, wherein the condensation is catalyzed by a prokaryotic HACL. The 2-hydroxyacyl-CoA can be converted to chemical products having broad applications by using enzyme catalysts. The combination of enzyme catalysts comprises novel biochemical reaction pathways that can be deployed either as polypeptides in a reaction buffer or genetically encoded in recombinant microorganisms.