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公开(公告)号:US20200347423A1
公开(公告)日:2020-11-05
申请号:US16878032
申请日:2020-05-19
申请人: Ramon Gonzalez , James Clomburg , Alexander Chou
发明人: Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号: C12P19/32 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/46 , C12N9/04 , C12N9/02 , C12N9/00 , C12P7/62 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/42 , C12P9/00 , C12P13/00 , C12P13/04
摘要: An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.
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公开(公告)号:US20190100741A1
公开(公告)日:2019-04-04
申请号:US15523284
申请日:2015-10-29
申请人: Ramon GONZALEZ , Alexander CHOU , James CLOMBURG
发明人: Ramon GONZALEZ , Alexander CHOU , James CLOMBURG
摘要: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.
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公开(公告)号:US06291254B1
公开(公告)日:2001-09-18
申请号:US09244616
申请日:1999-02-04
IPC分类号: G01L2166
CPC分类号: H01L22/34 , H01L2924/0002 , H01L2924/00
摘要: A method provides estimations of physical interconnect process parameter values in a process for manufacturing integrated circuits. The method includes fabricating test structures each providing a value of a measurable quantity corresponding to a value within a range of values of the physical interconnect process parameters. In some embodiments, the measured value is used to derive the values of the physical interconnect process parameters, either by a numerical method using a field solver, or by a closed-form solution. The values of physical interconnect process parameters involving physical dimensions are also obtained by measuring photomicrographs obtained using a scanning electron microscope from cross sections of test structures. In some embodiments, a family of test structures corresponding to a range of conductor widths and a range of spacings between conductors are measured.
摘要翻译: 一种方法提供了用于制造集成电路的过程中物理互连过程参数值的估计。 该方法包括制造测试结构,每个测试结构提供与物理互连过程参数的值的范围内的值相对应的可测量的值。 在一些实施例中,测量值用于通过使用场求解器的数值方法或通过闭式解决方案来导出物理互连过程参数的值。 涉及物理尺寸的物理互连工艺参数的值也可以通过测量使用扫描电子显微镜从测试结构的横截面获得的显微照片来获得。 在一些实施例中,测量对应于导体宽度的范围和导体之间的间隔范围的一系列测试结构。
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公开(公告)号:US6057171A
公开(公告)日:2000-05-02
申请号:US937393
申请日:1997-09-25
IPC分类号: H01L23/544 , H01L21/66 , G01R31/26
CPC分类号: H01L22/34 , H01L2924/0002
摘要: A method provides estimations of physical interconnect process parameter values in a process for manufacturing integrated circuits. The method includes fabricating test structures each providing a value of a measurable quantity corresponding to a value within a range of values of the physical interconnect process parameters. In some embodiments, the measured value is used to derive the values of the physical interconnect process parameters, either by a numerical method using a field solver, or by a closed-form solution. The values of physical interconnect process parameters involving physical dimensions are also obtained by measuring photomicrographs obtained using a scanning electron microscope from cross sections of test structures. In some embodiments, a family of test structures corresponding to a range of conductor widths and a range of spacings between conductors are measured.
摘要翻译: 一种方法提供了用于制造集成电路的过程中物理互连过程参数值的估计。 该方法包括制造测试结构,每个测试结构提供与物理互连过程参数的值的范围内的值相对应的可测量的值。 在一些实施例中,测量值用于通过使用场求解器的数值方法或通过闭式解决方案来导出物理互连过程参数的值。 涉及物理尺寸的物理互连工艺参数的值也可以通过测量使用扫描电子显微镜从测试结构的横截面获得的显微照片来获得。 在一些实施例中,测量对应于导体宽度的范围和导体之间的间隔范围的一系列测试结构。
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公开(公告)号:US11913049B2
公开(公告)日:2024-02-27
申请号:US16878032
申请日:2020-05-19
申请人: Ramon Gonzalez , James Clomburg , Alexander Chou
发明人: Ramon Gonzalez , James Clomburg , Alexander Chou
IPC分类号: C12N9/00 , C12P19/32 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/46 , C12N9/04 , C12N9/02 , C12P7/625 , C12N9/10 , C12N9/88 , C12N9/90 , C12P5/02 , C12P7/42 , C12P9/00 , C12P13/00 , C12P13/04
CPC分类号: C12P19/32 , C12N9/00 , C12N9/001 , C12N9/0006 , C12N9/0008 , C12N9/0077 , C12N9/13 , C12N9/88 , C12N9/90 , C12N9/93 , C12P5/02 , C12P5/026 , C12P7/02 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/625 , C12P9/00 , C12P13/001 , C12P13/04 , C12Y101/01 , C12Y101/03039 , C12Y102/01 , C12Y102/04001 , C12Y103/05001 , C12Y103/99 , C12Y108/01004 , C12Y114/15003 , C12Y203/01012 , C12Y203/01054 , C12Y207/01031 , C12Y208/03 , C12Y401/00 , C12Y401/01 , C12Y401/01047 , C12Y401/03024 , C12Y402/01 , C12Y402/01002 , C12Y402/01011 , C12Y501/99001 , C12Y501/99002 , C12Y504/02 , C12Y504/99 , C12Y504/99001 , C12Y504/99002 , C12Y602/01 , C12Y602/01005 , C12Y604/01003 , Y02E50/30
摘要: An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.
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公开(公告)号:US20220112477A1
公开(公告)日:2022-04-14
申请号:US17510641
申请日:2021-10-26
申请人: Ramon Gonzalez , Alexander Chou , James Clomburg
发明人: Ramon Gonzalez , Alexander Chou , James Clomburg
IPC分类号: C12N9/88 , C12N15/52 , C12P11/00 , C12P7/24 , C12P13/00 , C12P5/02 , C12P7/40 , C12P1/04 , C12P7/04 , C12N15/70 , C12P7/20
摘要: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.
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公开(公告)号:US11186834B2
公开(公告)日:2021-11-30
申请号:US15523284
申请日:2015-10-29
申请人: Ramon Gonzalez , Alexander Chou , James Clomburg
发明人: Ramon Gonzalez , Alexander Chou , James Clomburg
IPC分类号: C12P7/40 , C12P7/44 , C12P7/18 , C12N9/16 , C12N9/04 , C12N9/88 , C12N15/52 , C12P11/00 , C12P7/24 , C12P13/00 , C12P5/02 , C12P1/04 , C12P7/04 , C12N15/70 , C12P7/20
摘要: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.
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公开(公告)号:US06312963B1
公开(公告)日:2001-11-06
申请号:US09245812
申请日:1999-02-04
IPC分类号: G01L2166
CPC分类号: H01L22/34 , H01L2924/0002 , H01L2924/00
摘要: A method provides estimations of physical interconnect process parameter values in a process for manufacturing integrated circuits. The method includes fabricating test structures each providing a value of a measurable quantity corresponding to a value within a range of values of the physical interconnect process parameters. In some embodiments, the measured value is used to derive the values of the physical interconnect process parameters, either by a numerical method using a field solver, or by a closed-form solution. The values of physical interconnect process parameters involving physical dimensions are also obtained by measuring photomicrographs obtained using a scanning electron microscope from cross sections of test structures. In some embodiments, a family of test structures corresponding to a range of conductor widths and a range of spacings between conductors are measured.
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