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    Biosynthesis of polyketides
    2.
    发明授权
    Biosynthesis of polyketides 有权

    公开(公告)号：US11781120B2

    公开(公告)日：2023-10-10

    申请号：US16930220

    申请日：2020-07-15

    Applicant: Ramon Gonzalez

    Inventor： Ramon Gonzalez ,  Seokjung Cheong ,  James M. Clomburg

    IPC: C12N9/10 ,  C12P15/00 ,  C12P17/06

    CPC classification number: C12N9/1025 ,  C12P17/06 ,  C12Y203/01174

    Abstract: This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β-hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products.

    Modified fatty acid biosynthesis with ACP-dependent thiolases
    3.
    发明授权
    Modified fatty acid biosynthesis with ACP-dependent thiolases 有权

    公开(公告)号：US12163177B2

    公开(公告)日：2024-12-10

    申请号：US17713480

    申请日：2022-04-05

    Applicant: Ramon Gonzalez

    Inventor： Ramon Gonzalez ,  James M. Clomburg

    IPC: C12N1/20 ,  C12P7/6409

    Abstract: Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse β-oxidation cycle.

    Iterative platform for the synthesis of alpha functionalized products
    4.
    发明授权
    Iterative platform for the synthesis of alpha functionalized products 有权

    公开(公告)号：US11697830B2

    公开(公告)日：2023-07-11

    申请号：US16818642

    申请日：2020-03-13

    Applicant: Ramon Gonzalez ,  James M. Clomburg ,  Seokjung Cheong

    Inventor： Ramon Gonzalez ,  James M. Clomburg ,  Seokjung Cheong

    IPC: C12N9/02 ,  C12P7/42 ,  C12N9/00 ,  C12N9/10 ,  C12N9/04 ,  C12N9/16 ,  C12N9/88

    CPC classification number: C12P7/42 ,  C12N9/001 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01036 ,  C12Y102/0101 ,  C12Y103/01044 ,  C12Y402/01119

    Abstract: The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl-CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.

    Microaerobic cultures for converting glycerol to chemicals
    6.
    发明授权
    Microaerobic cultures for converting glycerol to chemicals 失效
    用于将甘油转化为化学物质的微生物培养物

    公开(公告)号：US08691552B2

    公开(公告)日：2014-04-08

    申请号：US13126499

    申请日：2009-10-28

    Applicant: Ramon Gonzalez ,  Paul Campbell

    Inventor： Ramon Gonzalez ,  Paul Campbell

    IPC: C12N1/20

    CPC classification number: C12P7/46 ,  C12P7/065 ,  C12P7/52 ,  C12P7/56 ,  C12P13/06 ,  C12R1/19 ,  Y02E50/17

    Abstract: Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.

    Abstract translation: 甘油或其他还原碳源可用作在某些微量厌氧条件下微生物生产化学产品的原料。 例如，这种生产可以在微需氧或微呼吸条件下发生，其中电子受体在反应中被消耗，就像加入它们一样快。 在这种反应中，反应产物至少与碳源一样还原。 此外，在这种反应期间，使用至少一些碳源来生成电池块。 此外，提供了具有修饰基因组的微生物用于实施本文的方法。

    Anaerobic fermentation of glycerol
    8.
    发明授权
    Anaerobic fermentation of glycerol 有权
    甘油的厌氧发酵

    公开(公告)号：US08334119B2

    公开(公告)日：2012-12-18

    申请号：US13347811

    申请日：2012-01-11

    Applicant: Ramon Gonzalez

    Inventor： Ramon Gonzalez

    IPC: C12P7/00

    CPC classification number: C12P7/065 ,  C12P3/00 ,  C12P7/40 ,  Y02E50/17

    Abstract: The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pII, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.

    Abstract translation: 本发明涉及在甘油底物上厌氧生长（发酵）培养细菌的合适培养条件。 该方法需要在含有高浓度甘油，中性至轻度酸性pII，低水平钾和磷酸盐的培养基中培养具有官能1,2-丙二醇途径和功能性II型甘油脱氢酶 - 二羟基丙酮激酶途径的细菌，以及 高水平的CO 2，使得甘油因此转化为所需的产物，例如乙醇，氢气，甲酸盐，琥珀酸盐或1,2-丙二醇。

    Table game
    9.
    发明授权
    Table game 失效
    桌面游戏

    公开(公告)号：US4679793A

    公开(公告)日：1987-07-14

    申请号：US838308

    申请日：1986-03-10

    Applicant: Ramon Gonzalez

    Inventor： Ramon Gonzalez

    IPC: A63F9/02 ,  A63F7/24

    CPC classification number: A63F9/02

    Abstract: A table game as provided to be played by up to four players and to simulate the action of a boxing contest, or the like. The game includes a flat board with players and markers and an adjustable pendulum used by the players to attempt to knock over the opponents' game pieces.

    Abstract translation: 由多达四名玩家提供的桌面游戏，并模拟拳击比赛的动作等。 该游戏包括一个带有玩家和标记的平板，以及玩家使用的可调节摆锤，试图敲击对手的游戏。

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