公开(公告)号：US20230342826A1

公开(公告)日：2023-10-26

申请号：US18053273

申请日：2022-11-07

Applicant: Promega Corporation

Inventor： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC: G06Q30/0283 ,  H04W4/35 ,  G06Q30/0601 ,  H04W4/02 ,  H04W4/38

CPC classification number: G06Q30/0283 ,  H04W4/35 ,  G06Q30/0631 ,  H04W4/02 ,  H04W4/38

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US11524946B2

公开(公告)日：2022-12-13

申请号：US16712098

申请日：2019-12-12

Applicant: Promega Corporation

Inventor： Wenhui Zhou ,  Kimberly K. Knoche ,  Douglas R. Storts ,  Min Zhou ,  Poncho Meisenheimer

IPC: C07D307/42 ,  C07C317/28 ,  C07D307/80 ,  C07D311/58 ,  C12N9/12 ,  C12P19/34

Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

公开(公告)号：US11493504B2

公开(公告)日：2022-11-08

申请号：US16851841

申请日：2020-04-17

Applicant: Promega Corporation

Inventor： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58 ,  A61K51/08

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US11192894B2

公开(公告)日：2021-12-07

申请号：US16725602

申请日：2019-12-23

Applicant: PROMEGA CORPORATION

Inventor： Mary Hall ,  Thomas Kirkland ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Anton Shakhmin ,  Joel R. Walker ,  Wenhui Zhou

IPC: C07D487/04 ,  G01N33/58 ,  C12Q1/66

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

公开(公告)号：US11136615B2

公开(公告)日：2021-10-05

申请号：US16053413

申请日：2018-08-02

Applicant: PROMEGA CORPORATION

Inventor： Ce Shi ,  Mary P. Hall ,  Thomas A. Kirkland ,  Lance P. Encell ,  Poncho Meisenheimer ,  Wenhui Zhou

IPC: C12Q1/66 ,  C07D253/06 ,  C07D417/04

Abstract: Disclosed herein are compositions and methods for stabilizing a benzothiazole luciferin analog such as D-luciferin and 6-amino-D-luciferin. The compositions may include the benzothiazole luciferin analog, a thionucleobase compound of formula (I), and a liquid medium, in which the thionucleobase is present in an amount effective to stabilize the luminogenic composition against decomposition. The methods provided herein may stabilize the benzothiazole luciferin analog against decomposition by contacting the benzothiazole luciferin analog with an effective amount of the thionucleobase compound in the presence of a liquid medium. Also provided herein is a kit containing the composition.

公开(公告)号：US20200239475A1

公开(公告)日：2020-07-30

申请号：US16725602

申请日：2019-12-23

Applicant: PROMEGA CORPORATION

Inventor： Mary Hall ,  Thomas Kirkland ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Anton Shakhmin ,  Joel R. Walker ,  Wenhui Zhou

IPC: C07D487/04 ,  C12Q1/66 ,  G01N33/58

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

公开(公告)号：US10577370B2

公开(公告)日：2020-03-03

申请号：US15829262

申请日：2017-12-01

Applicant: PROMEGA CORPORATION

Inventor： Mary Hall ,  Thomas Kirkland ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Anton Shakhmin ,  Joel R. Walker ,  Wenhui Zhou

IPC: C07D487/04 ,  G01N33/58 ,  C12Q1/66

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

公开(公告)号：US10400264B2

公开(公告)日：2019-09-03

申请号：US15829581

申请日：2017-12-01

Applicant: PROMEGA CORPORATION

Inventor： Lance P. Encell ,  Mary P. Hall ,  Michael Killoran ,  Thomas A. Kirkland ,  Poncho Meisenheimer ,  Ce Shi

IPC: C07D417/04 ,  C07D513/10 ,  C12Q1/66 ,  G01N21/76 ,  C07D513/04 ,  C07D277/66

Abstract: Described are luciferin analogs, methods for making the analogs, kits including the analogs, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

公开(公告)号：US10184936B2

公开(公告)日：2019-01-22

申请号：US15899219

申请日：2018-02-19

Applicant: Promega Corporation

Inventor： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20180313825A1

公开(公告)日：2018-11-01

申请号：US16035117

申请日：2018-07-13

Applicant: Promega Corporation

Inventor： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC: G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  G01N33/58 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542

CPC classification number: G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.