-
公开(公告)号：US20200292534A1
公开(公告)日：2020-09-17
申请号：US16851841
申请日：2020-04-17
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N33/533 , C07K14/435 , C12Q1/66 , C07K7/08 , C12N9/02 , G01N33/542 , G01N33/58 , A61K51/08
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
-
公开(公告)号：US10648971B2
公开(公告)日：2020-05-12
申请号：US16035117
申请日：2018-07-13
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N31/00 , G01N33/53 , G01N33/533 , C07K14/435 , C12Q1/66 , C07K7/08 , C12N9/02 , G01N33/542 , G01N33/58 , A61K51/08
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
-
公开(公告)号：US10288605B2
公开(公告)日：2019-05-14
申请号：US16023972
申请日：2018-06-29
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N31/00 , G01N33/53 , G01N33/533 , C07K14/435 , C12Q1/66 , C07K7/08 , A61K51/08 , C12N9/02 , G01N33/542 , G01N33/58
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
-
公开(公告)号：US20180196038A1
公开(公告)日：2018-07-12
申请号：US15899219
申请日：2018-02-19
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N33/533 , C07K14/435 , C12Q1/66 , C07K7/08 , G01N33/58 , A61K51/08 , C12N9/02 , G01N33/542
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
-
公开(公告)号：US09927430B2
公开(公告)日：2018-03-27
申请号：US14608910
申请日：2015-01-29
申请人： PROMEGA CORPORATION
发明人： Wenhui Zhou , Brock Binkowski , Hui Wang , Braeden Butler , Thomas Machleidt , Keith Wood
IPC分类号： C12Q1/66 , G01N33/68 , G01N33/542 , C07D487/04 , C07K5/093 , C07K5/113 , C07K5/072
CPC分类号： G01N33/542 , C07D487/04 , C07K5/06113 , C07K5/0819 , C07K5/1021 , C12Q1/66 , G01N33/6845
摘要： Provided are pro-substrates useful in assays of living cells. The pro-substrates can be used to detect the presence or absence of enzymes, such as luciferase, in living cells. The pro-substrates can be coelenterazine derivatives or analogs.
-
公开(公告)号：US20160282340A1
公开(公告)日：2016-09-29
申请号：US15073249
申请日：2016-03-17
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N33/533
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
摘要翻译： 本文提供了用于从两个或多个非发光（例如，基本上不发光的）肽和/或多肽单元组装生物发光复合物的组合物和方法。 特别地，通过与另一种互补的非发光肽的结构互补，赋予非发光多肽的生物发光活性。
-
公开(公告)号：US20150212078A1
公开(公告)日：2015-07-30
申请号：US14608910
申请日：2015-01-29
申请人： PROMEGA CORPORATION
发明人： Wenhui Zhou , Brock Binkowski , Hui Wang , Braeden Butler , Thomas Machleidt , Keith Wood
IPC分类号： G01N33/542 , C07K5/093 , C12Q1/66 , G01N33/68 , G01N33/58 , C07D487/04 , C07K5/113
CPC分类号： G01N33/542 , C07D487/04 , C07K5/06113 , C07K5/0819 , C07K5/1021 , C12Q1/66 , G01N33/6845
摘要： Provided are pro-substrates useful in assays of living cells. The pro-substrates can be used to detect the presence or absence of enzymes, such as luciferase, in living cells. The pro-substrates can be coelenterazine derivatives or analogues.
摘要翻译： 提供了可用于活细胞测定的前底物。 前底物可用于检测活细胞中酶，如荧光素酶的存在或不存在。 亲底物可以是肠丝氨酸衍生物或类似物。
-
公开(公告)号：US20140363375A1
公开(公告)日：2014-12-11
申请号：US14209610
申请日：2014-03-13
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N33/533 , A61K51/08
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
摘要翻译： 本文提供了用于从两个或多个非发光（例如，基本上不发光的）肽和/或多肽单元组装生物发光复合物的组合物和方法。 特别地，通过与另一种互补的非发光肽的结构互补，赋予非发光多肽的生物发光活性。
-
9.发明申请RECOGNITION OF CELLULAR TARGET BINDING BY A BIOACTIVE AGENT USING INTRACELLULAR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER 审中-公开通过生物活性剂使用细胞内生物发光共振能量转移识别细胞目标结合公开(公告)号：US20140194325A1
公开(公告)日：2014-07-10
申请号：US14104845
申请日：2013-12-12
申请人： Promega Corporation
发明人： Carolyn W. Hitko , Thomas Kirkland , Thomas Machleidt , Rachel Friedman Ohana , Matt Robers , Keith Wood
CPC分类号： G01N33/94 , G01N33/542 , G01N33/582
摘要： The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
摘要翻译： 本发明提供用于检测和分析生物活性剂与细胞靶标的细胞内结合的组合物和方法。 特别地，本文提供的是连接于荧光团的生物活性剂，与生物发光报道融合的细胞靶标，以及检测和分析生物活性剂与细胞靶标的相互作用的方法。
-
公开(公告)号：US20230341410A1
公开(公告)日：2023-10-26
申请号：US18150018
申请日：2023-01-04
申请人： Promega Corporation
发明人： Melanie Dart , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Matthew Robers , Brock F. Binkowski , Keith Wood , Ce Shi
IPC分类号： G01N33/68 , G01N33/569 , G01N21/64 , G01N33/58
CPC分类号： G01N33/6845 , G01N33/56916 , C12Y113/12007 , G01N21/6428 , G01N33/581 , G01N33/582 , G01N2021/6432 , G01N2021/6439 , G01N2333/90241 , G01N2500/04 , G01N2500/10
摘要： Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
-
-
-
-
-
-
-
-
-
搜索结果
53 条记录 (耗时 0.042 秒)
